US5551422A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and apparatus for analytical determination of glucose in a biological matrix
Assignee: Roche Diagnostics GmbH
•
Filing: 1994-07-06
•
Granted: 1996-09-03
•
Expires: 2014-07-06
Abstract
Abstract A method for determining glucose concentration in a biological matrix includes the steps of performing at least two detection measurements. In each detection measurement light is irradiated at a defined irradiation site as primary light into the biological matrix, through a boundary surface thereof. The light is propagated along a light path within the biological matrix. An intensity of the light is measured as the light emerges as secondary light through a defined detection site of the boundary surface. At least one of the detection measurements is a spatially resolved measurement of multiply scattered light. The detection site is located relative to the irradiation site such that light which was multiply scattered at scattering centers in the biological matrix is detected. The light paths of the at least two detection measurements within the biological matrix are different. Glucose concentration is then derived from the dependence of the intensity of the secondary light from the relative position of the irradiation site and the detection site.
Inventors
- 👤 Jan H. Simonsen
- 👤 Dirk Boecker
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 02:55
CPC Classifications
A; A61; A61B; A61B5/00; A61B5/145; A61B5/1455; A61B5/14532; A61B5/68; A61B5/6801; A61B5/6813; A61B5/6825; A61B5/6826; A61B5/683; A61B5/6832; A61B5/6833; A61B5/6838; A61B2562/00; A61B2562/02; A61B2562/0233; A61B2562/04; A61B2562/046
Claims Analysis
Total Claims: 103
Claim 1
Independent Claim
223 words
A method for determination of glucose concentration in a biological matrix, said method comprising the steps of: performing at least two detection measurements, each detection measurement comprising the further steps of irradiating light at a first irradiation site as primary light into the biological matrix through a boundary surface thereof, said light being propagated along a light path within the biological matrix, and measuring an intensity of the light as the light emerges from the biological matrix as secondary light through a first detection site of the boundary surface thereof; wherein at least one of said at least two detection measurements is a spatially resolved measurement of multiply scattered light, wherein the primary light is irradiated into the biological matrix at the first irradiation site, the intensity of the secondary light emerging from the biological matrix at the first detection site is measured, and wherein the first detection site is located relative to the first irradiation site such that light which was multiply scattered by scattering centers in the biological matrix is detected, and wherein the light paths of said at least two detection measurements are different, and deriving the glucose concentrations from the dependence of the intensity of the secondary light from the relative position of the first irradiation site and the first detection site in said at least two detection measurements.
Claim 2
Independent Claim
223 words
A method for determination of glucose concentration in a biological matrix, said method comprising the steps of: performing at least two detection measurements, each detection measurement comprising the further steps of irradiating light at a first irradiation site as primary light into the biological matrix through a boundary surface thereof, said light being propagated along a light path within the biological matrix, and measuring an intensity of the light as the light emerges from the biological matrix as secondary light through a first detection site of the boundary surface thereof; wherein at least one of said at least two detection measurements is a spatially resolved measurement of multiply scattered light, wherein the primary light is irradiated into the biological matrix at the first irradiation site, the intensity of the secondary light emerging from the biological matrix at the first detection site is measured, and wherein the first detection site is located relative to the first irradiation site such that light which was multiply scattered by scattering centers in the biological matrix is detected, and wherein the light paths of said at least two detection measurements are different, and deriving the glucose concentrations from the dependence of the intensity of the secondary light from the relative position of the first irradiation site and the first detection site in said at least two detection measurements.
Claim 3
Dependent Claim
73 words
A method according to claim 1, wherein said at least two detection measurements are spatially resolved measurements of multiply scattered light, wherein the intensities of the secondary light emerging from the biological matrix at the first detection site are measured, and wherein the first detection site is located relative to the first irradiation site such that light which was multiply scattered by scattering centers in the biological matrix is detected for each measurement.
Claim 4
Dependent Claim
26 words
A method according to claim 2, wherein a plurality of spatially resolved measurements of multiply scattered light are performed with a same wavelength of primary light.
Claim 5
Dependent Claim
63 words
A method according to claim 2, wherein the first irradiation site and the first detection site are separated by a first distance in a first spatially resolved measurement of multiply scattered light, and the first irradiation site and the first detection site are separated by a second distance, different from the first distance, in a second spatially resolved measurement of multiply scattered light.
Claim 6
Dependent Claim
61 words
A method according to claim 4, wherein a difference between the first and second measurement distances is selected such that the intensity of the secondary light in the first spatially resolved measurement of multiply scattered light and the intensity of the secondary light in the second spatially resolved measurement of multiply scattered light are in a ratio of at least 13:1.
Claim 7
Dependent Claim
19 words
A method according to claim 5, wherein said difference is selected such that said ratio is at least 5:1.
Claim 8
Dependent Claim
19 words
A method according to claim 5, wherein said difference is selected such that said ratio is at least 10:1.
Claim 9
Dependent Claim
85 words
A method according to claim 2, wherein said detection measurements are performed such that in a detection area, a plurality of sub-areas are provided as detection sites for measurement of the intensity of the secondary light emerging at the plurality of detection sites, said intensity being measured as a function of the distance of each of the detection sites from the first irradiation site, said plurality of detection sites being selected such that a plurality of spatially resolved measurements of multiply scattered light are performed.
Claim 10
Dependent Claim
32 words
A method according to claim 8, wherein a density of the plurality of detection sites in the detection area, in at least one dimension, is at least two detection sites per centimeter.
Claim 11
Dependent Claim
35 words
A method according to claim 9, wherein the intensity of the light emerging from the biological matrix in the detection area is measured in a spatially resolved manner by a two-dimensional array of photosensitive elements.
Claim 12
Dependent Claim
32 words
A method according to claim 8, wherein a density of the plurality of detection sites in the detection area, in at least one dimension, is at least four detection sites per centimeter.
Claim 13
Dependent Claim
46 words
A method according to claim 2, wherein, in the evaluation step, absorption and scattering are separated as factors influencing the detected intensity by evaluating the intensity profile of the secondary light as a function of the measurement distance of the detection site from the irradiation site.
Claim 14
Dependent Claim
93 words
A method according to claim 2, wherein a first spatially resolved measurement is performed by irradiating light at the first irradiation site and detecting light at the first detection site with a first measurement distance therebetween, and wherein a second spatially resolved measurement is performed by irradiating light at a second irradiation site and detecting light at a second detection site with the first measuring distance therebetween, wherein at least one of the second irradiation site and the second detection site is different than the respective first irradiation site and first detection site.
Claim 15
Dependent Claim
31 words
A method according to claim 13, comprising selecting said second irradiation site and said second detection site to be different from the first irradiation site and the first detection site, respectively.
Claim 16
Dependent Claim
123 words
A method according to claim 13, wherein the primary light is irradiated in the first irradiation site, the second irradiation site, and a third irradiation site, wherein said first, second and third irradiation sites are different, and the secondary light is detected in the first detection site, the second detection site, and a third detection site, wherein said first, second, and third detection sites are different, wherein the irradiation sites and detection sites are combined in such a way such that at least three spatially resolved measurements of multiply scattered light having at least one of a different irradiation site and a different detection site, are made for each of at least two measurement distances between the irradiation sites and the detection sites.
Claim 17
Dependent Claim
52 words
A method according to claim 15, wherein the first, second and third irradiation sites are disposed on a straight line, and at least six detection sites are provided, said six detection sites being arranged in a mirror-symmetrical configuration in pairs on either side of the straight line created by the irradiation sites.
Claim 18
Dependent Claim
53 words
A method according to claim 16, wherein the irradiation sites arranged on the straight line are equidistant from each other, and the at least six detection sites are arranged in a circular pattern around the second irradiation site, which is arranged at a central location between the first and the third irradiation site.
Claim 19
Dependent Claim
46 words
A method according to claim 15, wherein the first, second and third detection sites are disposed in a straight line configuration, and at least six irradiation sites are arranged mirror symmetrically in pairs on either side of the straight line created by the three detection sites.
Claim 20
Dependent Claim
53 words
A method according to claim 18, wherein the detection sites are configured to be equidistant from each other on the straight line, and wherein the irradiation sites are arranged in a circular pattern around the second detection site, which is located in a central location between the first and the third detection sites.
Claim 21
Dependent Claim
40 words
A method according to claim 1, wherein in the at least one spatially resolved measurement of multiply scattered light, a measured change in intensity of the secondary light per 100 mg/dl change in the glucose concentration is more than 0.5%.
Claim 22
Dependent Claim
61 words
A method according to claim 1, wherein said detection measurements are performed such that a measurement distance between a center of the first irradiation site and a center of the first detection site in the at least one spatially resolved measurement of multiply scattered light is at least ten times a mean free path length of photons in the biological matrix.
Claim 23
Dependent Claim
50 words
A method according to claim 1, wherein said detection measurements are performed such that a distance between a center of the first irradiation site and a center of the first detection site in the at least one spatially resolved measurement of multiply scattered light is no greater than 30 mm.
Claim 24
Dependent Claim
50 words
A method according to claim 1, wherein said detection measurements are performed such that a distance between a center of the first irradiation site and a center of the first detection site in the at least one spatially resolved measurement of multiply scattered light is no greater than 15 mm.
Claim 25
Dependent Claim
50 words
A method according to claim 1, wherein said detection measurements are performed such that a distance between a center of the first irradiation site and a center of the first detection site in the at least one spatially resolved measurement of multiply scattered light is no greater than 10 mm.
Claim 26
Dependent Claim
27 words
A method according to claim 1, wherein said detection measurements are performed such that a wavelength of the primary light is between 400 nm and 2500 nm.
Claim 27
Dependent Claim
19 words
A method according to claim 25, wherein the wavelength of the primary light is between 400 and 600 nm.
Claim 28
Dependent Claim
19 words
A method according to claim 25, wherein the wavelength of the primary light is between 750 and 920 nm.
Claim 29
Dependent Claim
19 words
A method according to claim 27, wherein the wavelength of the primary light is between 780 and 825 nm.
Claim 30
Dependent Claim
19 words
A method according to claim 27, wherein the wavelength of the primary light is between 850 and 900 nm.
Claim 31
Dependent Claim
19 words
A method according to claim 25, wherein the wavelength of the primary light is between 1,050 and 1,350 nm.
Claim 32
Dependent Claim
19 words
A method according to claim 25, wherein the wavelength of the primary light is between 1,200 and 1,300 nm.
Claim 33
Dependent Claim
19 words
A method according to claim 25, wherein the wavelength of the primary light is between 1,600 and 1,800 nm.
Claim 34
Dependent Claim
19 words
A method according to claim 32, wherein the wavelength of the primary light is between 1,630 and 1,770 nm.
Claim 35
Dependent Claim
19 words
A method according to claim 33, wherein the wavelength of the primary light is between 1,630 and 1,670 nm.
Claim 36
Dependent Claim
19 words
A method according to claim 33, wherein the wavelength of the primary light is between 1,730 and 1,770 nm.
Claim 37
Dependent Claim
41 words
A method according to claim 1, wherein said detection measurements are performed such that a wavelength of the primary light is selected to be in a spectral region wherein glucose concentration has a minimal effect on absorption of the primary light.
Claim 38
Dependent Claim
48 words
A method according to claim 1, wherein in the at least one spatially resolved measurement of multiply scattered light, a dimension of the first detection site in a spatial direction, which is defined by a shortest distance to the first irradiation site, is no more than 2 mm.
Claim 39
Dependent Claim
48 words
A method according to claim 1, wherein in the at least one spatially resolved measurement of multiply scattered light, a dimension of the first detection site in a spatial direction, which is defined by a shortest distance to the first irradiation site, is no more than 1 mm.
Claim 40
Dependent Claim
52 words
A method according to claim 1, wherein in the at least one spatially resolved measurement of multiply scattered light, a dimension of the first irradiation site in a spatial direction, which is defined by a shortest distance to the first detection site, is an average distance of no more than 2 mm.
Claim 41
Dependent Claim
52 words
A method according to claim 1, wherein in the at least one spatially resolved measurement of multiply scattered light, a dimension of the first irradiation site in a spatial direction, which is defined by a shortest distance to the first detection site, is an average distance of no more than 1 mm.
Claim 42
Dependent Claim
57 words
A method according to claim 1, wherein in the at least one spatially resolved measurement of multiply scattered light, the first irradiation site and the first detection site are disposed on a same boundary surface of the biological matrix, such that radiation which is diffusely reflected from the biological matrix can be measured at the detection site.
Claim 43
Dependent Claim
39 words
A method according to claim 1, wherein in the at least one spatially resolved measurement of multiply scattered light, the first detection site has a long, narrow configuration such that a length is significantly greater than a width thereof.
Claim 44
Dependent Claim
16 words
A method according to claim 42, wherein said long, narrow detection field has a curved configuration.
Claim 45
Dependent Claim
40 words
A method according to claim 43, wherein in the at least one spatially resolved measurement of multiply scattered light, the first irradiation site has a long, narrow configuration such that a length thereof is substantially greater than a width thereof.
Claim 46
Dependent Claim
14 words
A method according to claim 44, wherein said irradiation site has a curved configuration.
Claim 47
Dependent Claim
40 words
A method according to claim 1, wherein in the at least one spatially resolved measurement of multiply scattered light, the first irradiation site has a long, narrow configuration such that a length thereof is substantially greater than a width thereof.
Claim 48
Dependent Claim
14 words
A method according to claim 46, wherein said irradiation site has a curved configuration.
Claim 49
Dependent Claim
34 words
A method according to claim 1, further comprising a step of measuring a temperature at the detection site, with a result of said measurement being incorporated into the step of deriving the glucose concentration.
Claim 50
Dependent Claim
20 words
A method according to claim 1, wherein a temperature at the detection site is maintained to be a constant temperature.
Claim 51
Dependent Claim
16 words
A method according to claim 1, comprising selecting the biological matrix to be a biological fluid.
Claim 52
Dependent Claim
14 words
A method according to claim 50, comprising selecting said biological fluid to be blood.
Claim 53
Dependent Claim
16 words
A method according to claim 1, comprising selecting the biological matrix to be a biological tissue.
Claim 54
Dependent Claim
51 words
A method according to claim 52, comprising selecting the biological tissue to be cutaneous tissue of a human body, said cutaneous tissue being located in at least one area of a group of areas consisting of fingertips, trunk, nail bed, lip, tongue, scleral tissue, and an inside of an upper arm.
Claim 55
Independent Claim
305 words
A method for spectral analytical determination of an analyte in a biological matrix, said method comprising the steps of: performing at least two detection measurements, each of said at least two detection measurements being for measurement of spectral dependence of absorption of the analyte, said detection measurements being at at least two different wavelengths, each detection measurement comprising the further steps of irradiating light at a first irradiation site as a primary light into the biological matrix through a boundary surface thereof, said light being propagated along a light path within the biological matrix, and measuring an intensity of the light as the light emerges from the biological matrix as secondary light through a first detection site of the boundary surface thereof; performing an evaluation step wherein the analyte concentration is derived from the measured intensities of the detection measurements by means of an evaluation algorithm and a calibration; wherein at least one of said at least two detection measurements is a spatially resolved measurement of multiply scattered light wherein the primary light is irradiated into the biological matrix by the first irradiation site, the intensity of the secondary light emerging from the biological matrix at the first detection site is measured, and wherein the first detection site is located relative to the first irradiation site such that light which was multiply scattered by scattering centers in the biological matrix is detected, and wherein the light paths of said at least two detection measurements within the biological matrix are different, and deriving a correction value for the evaluation algorithm for correction due to changes in an optical path length in said at least two detection measurements, said correction value being derived from the dependence of the intensity of the secondary light on the relative position of the first irradiation site and the first detection site.
Claim 56
Independent Claim
305 words
A method for spectral analytical determination of an analyte in a biological matrix, said method comprising the steps of: performing at least two detection measurements, each of said at least two detection measurements being for measurement of spectral dependence of absorption of the analyte, said detection measurements being at at least two different wavelengths, each detection measurement comprising the further steps of irradiating light at a first irradiation site as a primary light into the biological matrix through a boundary surface thereof, said light being propagated along a light path within the biological matrix, and measuring an intensity of the light as the light emerges from the biological matrix as secondary light through a first detection site of the boundary surface thereof; performing an evaluation step wherein the analyte concentration is derived from the measured intensities of the detection measurements by means of an evaluation algorithm and a calibration; wherein at least one of said at least two detection measurements is a spatially resolved measurement of multiply scattered light wherein the primary light is irradiated into the biological matrix by the first irradiation site, the intensity of the secondary light emerging from the biological matrix at the first detection site is measured, and wherein the first detection site is located relative to the first irradiation site such that light which was multiply scattered by scattering centers in the biological matrix is detected, and wherein the light paths of said at least two detection measurements within the biological matrix are different, and deriving a correction value for the evaluation algorithm for correction due to changes in an optical path length in said at least two detection measurements, said correction value being derived from the dependence of the intensity of the secondary light on the relative position of the first irradiation site and the first detection site.
Claim 57
Dependent Claim
73 words
A method according to claim 54, wherein said at least two detection measurements are spatially resolved measurements of multiply scattered light, wherein the intensities of the secondary light emerging from the biological matrix at the first detection site are measured, and wherein the first detection site is located relative to the first irradiation site such that light which was multiply scattered at scattering centers in the biological matrix is detected for each measurement.
Claim 58
Dependent Claim
26 words
A method according to claim 55, wherein a plurality of spatially resolved measurements of multiply scattered light are performed with a same wavelength of primary light.
Claim 59
Dependent Claim
63 words
A method according to claim 55, wherein the first irradiation site and the first detection site are separated by a first distance in a first spatially resolved measurement of multiply scattered light, and the first irradiation site and the first detection site are separated by a second distance, different from the first distance, in a second spatially resolved measurement of multiply scattered light.
Claim 60
Dependent Claim
61 words
A method according to claim 57, wherein a difference between the first and second measurement distances is selected such that the intensity of the secondary light in the first spatially resolved measurement of multiply scattered light and the intensity of the secondary light in the second spatially resolved measurement of multiply scattered light are in a ratio of at least 13:1.
Claim 61
Dependent Claim
19 words
A method according to claim 58, wherein said difference is selected such that said ratio is at least 5:1.
Claim 62
Dependent Claim
19 words
A method according to claim 58, wherein said difference is selected such that said ratio is at least 10:1.
Claim 63
Dependent Claim
85 words
A method according to claim 55, wherein said detection measurements are performed such that in a detection area, a plurality of sub-areas are provided as detection sites for measurement of the intensity of the secondary light emerging at the plurality of detection sites, said intensity being measured as a function of the distance of each of the detection sites from the first irradiation site, said plurality of detection sites being selected such that a plurality of spatially resolved measurements of multiply scattered light are performed.
Claim 64
Dependent Claim
32 words
A method according to claim 61, wherein a density of the plurality of detection sites in the detection area, in at least one dimension, is at least two detection sites per centimeter.
Claim 65
Dependent Claim
32 words
A method according to claim 61, wherein a density of the plurality of detection sites in the detection area, in at least one dimension, is at least four detection sites per centimeter.
Claim 66
Dependent Claim
35 words
A method according to claim 61, wherein the intensity of the light emerging from the biological matrix in the detection area is measured in a spatially resolved manner by a two-dimensional array of photosensitive elements.
Claim 67
Dependent Claim
94 words
A method according to claim 55, wherein a first spatially resolved measurement is performed by irradiating light at the first irradiation site and detecting light at the first detection site with a first measurement distance therebetween, and wherein a second spatially resolved measurement is performed by irradiating light at a second irradiation site and by detecting light at a second detection site with the first measuring distance therebetween, wherein at least one of the second irradiation site and the second detection site is different than the respective first irradiation site and first detection site.
Claim 68
Dependent Claim
31 words
A method according to claim 65, comprising selecting said second irradiation site and said second detection site to be different from the first irradiation site and the first detection site, respectively.
Claim 69
Dependent Claim
123 words
A method according to claim 65, wherein the primary light is irradiated in the first irradiation site, the second irradiation site, and a third irradiation site, wherein said first, second and third irradiation sites are different, and the secondary light is detected in the first detection site, the second detection site, and a third detection site, wherein said first, second, and third detection sites are different, wherein the irradiation sites and detection sites are combined in such a way such that at least three spatially resolved measurements of multiply scattered light having at least one of a different irradiation site and a different detection site, are made for each of at least two measurement distances between the irradiation sites and the detection sites.
Claim 70
Dependent Claim
52 words
A method according to claim 67, wherein the first, second and third irradiation sites are disposed on a straight line, and at least six detection sites are provided, said six detection sites being arranged in a mirror-symmetrical configuration in pairs on either side of the straight line created by the irradiation sites.
Claim 71
Dependent Claim
53 words
A method according to claim 68, wherein the irradiation sites arranged on the straight line are equidistant from each other, and the at least six detection sites are arranged in a circular pattern around the second irradiation site, which is arranged at a central location between the first and the third irradiation site.
Claim 72
Dependent Claim
46 words
A method according to claim 67, wherein the first, second and third detection sites are disposed in a straight line configuration, and at least six irradiation sites are arranged mirror symmetrically in pairs on either side of the straight line created by the three detection sites.
Claim 73
Dependent Claim
53 words
A method according to claim 70, wherein the detection sites are configured to be equidistant from each other on the straight line, and wherein the irradiation sites are arranged in a circular pattern around the second detection site, which is located in a central location between the first and the third detection sites.
Claim 74
Dependent Claim
39 words
A method according to claim 54, wherein in the at least one spatially resolved measurement of multiply scattered light, a measured change in intensity of the secondary light per 100 mg/dl change in glucose concentration is more than 0.5%.
Claim 75
Dependent Claim
61 words
A method according to claim 54, wherein said detection measurements are performed such that a measurement distance between a center of the first irradiation site and a center of the first detection site in the at least one spatially resolved measurement of multiply scattered light is at least ten times a mean free path length of photons in the biological matrix.
Claim 76
Dependent Claim
50 words
A method according to claim 54, wherein said detection measurements are performed such that a distance between a center of the first irradiation site and a center of the first detection site in the at least one spatially resolved measurement of multiply scattered light is no greater than 30 mm.
Claim 77
Dependent Claim
50 words
A method according to claim 54, wherein said detection measurements are performed such that a distance between a center of the first irradiation site and a center of the first detection site in the at least one spatially resolved measurement of multiply scattered light is no greater than 15 mm.
Claim 78
Dependent Claim
50 words
A method according to claim 54, wherein said detection measurements are performed such that a distance between a center of the first irradiation site and a center of the first detection site in the at least one spatially resolved measurement of multiply scattered light is no greater than 10 mm.
Claim 79
Dependent Claim
26 words
A method according to claim 54, wherein said detection measurements are performed such that wavelengths of the primary light are between 400 nm and 2500 nm.
Claim 80
Dependent Claim
19 words
A method according to claim 77, wherein the wavelength of the primary light is between 400 and 600 nm.
Claim 81
Dependent Claim
19 words
A method according to claim 77, wherein the wavelength of the primary light is between 750 and 920 nm.
Claim 82
Dependent Claim
19 words
A method according to claim 79, wherein the wavelength of the primary light is between 780 and 825 nm.
Claim 83
Dependent Claim
19 words
A method according to claim 79, wherein the wavelength of the primary light is between 850 and 900 nm.
Claim 84
Dependent Claim
19 words
A method according to claim 77, wherein the wavelength of the primary light is between 1,050 and 1,350 nm.
Claim 85
Dependent Claim
19 words
A method according to claim 77, wherein the wavelength of the primary light is between 1,200 and 1,300 nm.
Claim 86
Dependent Claim
19 words
A method according to claim 77, wherein the wavelength of the primary light is between 1,600 and 1,800 nm.
Claim 87
Dependent Claim
19 words
A method according to claim 84, wherein the wavelength of the primary light is between 1,630 and 1,770 nm.
Claim 88
Dependent Claim
19 words
A method according to claim 85, wherein the wavelength of the primary light is between 1,630 and 1,670 nm.
Claim 89
Dependent Claim
19 words
A method according to claim 85, wherein the wavelength of the primary light is between 1,730 and 1,770 nm.
Claim 90
Dependent Claim
41 words
A method according to claim 54, wherein said detection measurements are performed such that a wavelength of the primary light is selected to be in a spectral region wherein glucose concentration has a minimal effect on absorption of the primary light.
Claim 91
Dependent Claim
48 words
A method according to claim 54, wherein in the at least one spatially resolved measurement of multiply scattered light, a dimension of the first detection site in a spatial direction, which is defined by a shortest distance to the first irradiation site, is no more than 2 mm.
Claim 92
Dependent Claim
48 words
A method according to claim 54, wherein in the at least one spatially resolved measurement of multiply scattered light, a dimension of the first detection site in a spatial direction, which is defined by a shortest distance to the first irradiation site, is no more than 1 mm.
Claim 93
Dependent Claim
52 words
A method according to claim 54, wherein in the at least one spatially resolved measurement of multiply scattered light, a dimension of the first irradiation site in a spatial direction, which is defined by a shortest distance to the first detection site, is an average distance of no more than 2 mm.
Claim 94
Dependent Claim
52 words
A method according to claim 54, wherein in the at least one spatially resolved measurement of multiply scattered light, a dimension of the first irradiation site in a spatial direction, which is defined by a shortest distance to the first detection site, is an average distance of no more than 1 mm.
Claim 95
Dependent Claim
57 words
A method according to claim 54, wherein in the at least one spatially resolved measurement of multiply scattered light, the first irradiation site and the first detection site are disposed on a same boundary surface of the biological matrix, such that radiation which is diffusely reflected from the biological matrix can be measured at the detection site.
Claim 96
Dependent Claim
39 words
A method according to claim 54, wherein in the at least one spatially resolved measurement of multiply scattered light, the first detection site has a long, narrow configuration such that a length is significantly greater than a width thereof.
Claim 97
Dependent Claim
16 words
A method according to claim 94, wherein said long, narrow detection field has a curved configuration.
Claim 98
Dependent Claim
30 words
A method according to claim 54, further comprising a step of measuring a temperature at the detection site, with a result of said measurement being incorporated into the evaluation algorithm.
Claim 99
Dependent Claim
20 words
A method according to claim 54, wherein a temperature at the detection site is maintained to be a constant temperature.
Claim 100
Dependent Claim
16 words
A method according to claim 54, comprising selecting the biological matrix to be a biological fluid.
Claim 101
Dependent Claim
14 words
A method according to claim 98, comprising selecting said biological fluid to be blood.
Claim 102
Dependent Claim
16 words
A method according to claim 55, comprising selecting the biological matrix to be a biological tissue.
Claim 103
Dependent Claim
51 words
A method according to claim 100, comprising selecting the biological tissue to be cutaneous tissue of a human body, said cutaneous tissue being located in at least one area of a group of areas consisting of fingertips, trunk, nail bed, lip, tongue, scleral tissue, and an inside of an upper arm.
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| 3.5 Years (Year 4) | 1999-09-03 | 2000-03-04 | 2000-09-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-09-03 | 2004-03-04 | 2004-09-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-09-03 | 2008-03-04 | 2008-09-03 | $7700.00 | Window Closed / Lapsed |