EP0193868A2
Expired / Lapsed
EP
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Measurement of optical density via nephelometry
Assignee: Abbott Laboratories
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Filing: 1986-02-27
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Granted: —
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Expires: 2006-02-27
Abstract
Abstract 57 A method for measuring the formation of optical density at 350 to 700 nm in a material is disclosed. The method comprises directing through a solution of the material with light-scattering means suspended therein a beam of light having a wavelength in the range of 350 to 700 nm at which the material absorbs light, and measuring the scattered intensity of the light leaving the solution at a given angle.
Inventors
- 👤 John G. Pumphrey
- 👤 Robert E. Shanks
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-25 04:29
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/17; G01N21/47; G01N21/49; G01N2021/1748; G01N2021/4704; G01N9/00; G01N9/24
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
68 words
A method for measuring the formation of optical density at 350 to 700 nm in a material, said method comprising directing through a solution of the material with light-scattering means suspended therein a beam of light having a wavelength in the range of 350 to 700 nm at which the material absorbs light, and measuring the scattered intensity of the light leaving the solution at a given angle.
Claim 2
Independent Claim
68 words
A method for measuring the formation of optical density at 350 to 700 nm in a material, said method comprising directing through a solution of the material with light-scattering means suspended therein a beam of light having a wavelength in the range of 350 to 700 nm at which the material absorbs light, and measuring the scattered intensity of the light leaving the solution at a given angle.
Claim 3
Dependent Claim
103 words
A method as claimed in claim 1 for measuring the formation of optical density at 350 to 700 nm in a material, which method includes the additional steps of directing a beam of light having characteristics at least substantially the same as those of the beam which is. directed through the solution of the material with light-scattering means suspended therein through a blank which, optically, is substantially identical to the solution with suspended light-scattering means except that neither the solution of the material nor the light-scattering means is present, and measuring the intensity of the light leaving the blank at the given angle.
Claim 4
Independent Claim
138 words
A method for measuring the formation of optical density at 350 to 700 nm in a material, said method comprising directing through a first sample which includes a solution of the material with light-scattering means suspended therein, a first beam of light having a wavelength in the range of 350 to 700 nm at which the material absorbs light, measuring the scattered intensity of the light leaving the first sample at a given angle, directing a beam of light having characteristics at least substantially the same as those of the first beam through a second sample which, optically, is substantially identical to the first sample except that the solution of the material which absorbs light at the indicated frequency is not present, and measuring the scattered intensity of the light leaving the second sample at the given angle.
Claim 5
Independent Claim
138 words
A method for measuring the formation of optical density at 350 to 700 nm in a material, said method comprising directing through a first sample which includes a solution of the material with light-scattering means suspended therein, a first beam of light having a wavelength in the range of 350 to 700 nm at which the material absorbs light, measuring the scattered intensity of the light leaving the first sample at a given angle, directing a beam of light having characteristics at least substantially the same as those of the first beam through a second sample which, optically, is substantially identical to the first sample except that the solution of the material which absorbs light at the indicated frequency is not present, and measuring the scattered intensity of the light leaving the second sample at the given angle.
Claim 6
Dependent Claim
87 words
A method as claimed in claim 3 for measuring the formation of optical density at 350 to 700 nm in a material, which method includes the additional steps of directing a beam of light having characteristics at least substantially the same as those of the first beam through a blank which, optically, is substantially identical to the first sample except that neither the solution of the material nor the light-scattering means is present, and measuring the intensity of the light leaving the blank at the given angle.
Claim 7
Independent Claim
177 words
A method for measuring the formation of optical density at 350 to 700 nm, said method comprising placing in a sample container which is transparent to energy having a wavelength between 350 and 700 nm a suspension of a given weight per mL of light-scattering means, energizing a source to direct a beam of light having a wavelength in the range of 350 to 700 nm to which the sample container is transparent along a given path through the suspension, measuring the scattered intensity of the light leaving the suspension at a given angle, adding to the suspension in the sample container a solution of a material which absorbs light of the frequency furnished by the source and an additional quantity of the light-scattering means to produce a solution of the material with the given weight per mL of the light-scattering means suspended therein, energizing the source to direct a beam of light along substantially the same given path through the suspension, and measuring the scattered intensity of the light leaving the suspension at the given angle.
Claim 8
Independent Claim
177 words
A method for measuring the formation of optical density at 350 to 700 nm, said method comprising placing in a sample container which is transparent to energy having a wavelength between 350 and 700 nm a suspension of a given weight per mL of light-scattering means, energizing a source to direct a beam of light having a wavelength in the range of 350 to 700 nm to which the sample container is transparent along a given path through the suspension, measuring the scattered intensity of the light leaving the suspension at a given angle, adding to the suspension in the sample container a solution of a material which absorbs light of the frequency furnished by the source and an additional quantity of the light-scattering means to produce a solution of the material with the given weight per mL of the light-scattering means suspended therein, energizing the source to direct a beam of light along substantially the same given path through the suspension, and measuring the scattered intensity of the light leaving the suspension at the given angle.
Claim 9
Dependent Claim
70 words
A method as claimed in claim 5 for measuring the formation of optical density at 350 to 700 nm in a material, which method includes the additional steps of directing a beam of light from the source through a blank which, optically, is substantially identical to the sample except that the light-scattering means is not present, and measuring the intensity of the light leaving the blank at the given angle.
Claim 10
Dependent Claim
89 words
A method as claimed in claim 5 wherein the -suspension of light scattering means which is placed in the sample container includes a dissolved material which is reactive with a second material to produce a reaction product that is soluble in the dispersion and absorbs light of the frequency furnished by the source, and wherein the second material and additional light-scattering means are added to the sample container after the first measurement of scattered light intensity to produce the reaction product before the second measurement of scattered light intensity.
References Cited (Backward Citations)
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