US5489678A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Photolabile nucleoside and peptide protecting groups
Assignee: Affymetrix Inc
•
Filing: 1995-02-16
•
Granted: 1996-02-06
•
Expires: 2015-02-16
Abstract
Abstract A synthetic strategy for the creation of large scale chemical diversity. Solid-phase chemistry, photolabile protecting groups, and photolithography are used to achieve light-directed spatially-addressable parallel chemical synthesis. Binary masking techniques are utilized in one embodiment. A reactor system, photoremovable protective groups, and improved data collection and handling techniques are also disclosed. A technique for screening linker molecules is also provided.
Inventors
- 👤 Stephen P. A. Fodor
- 👤 Lubert Stryer
- 👤 James L. Winkler
- 👤 Christopher P. Holmes
- 👤 Dennis W. Solas
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 16:14
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/645; G01N21/6452; B; B01; B01J; B01J19/00; B01J19/0046; B82; B82Y; B82Y10/00; B82Y30/00; C; C07; C07C; C07C229/00; C07C229/02; C07C229/04; C07C229/06; C07C229/10; C07C229/14; C07C229/16; C07D; C07D263/00; C07D263/02; C07D263/30; C07D263/34; C07D263/44; C07D317/00; C07D317/08; C07D317/44; C07D317/46; C07D317/48; C07D317/62; C07H; C07H19/00; C07H19/02; C07H19/04; C07H19/06; C07H19/073; C07H19/16; C07H19/173; C07H21/00; C07H21/04; C07K; C07K1/00; C07K1/04; C07K1/042; C07K1/045; C07K1/047; C07K1/06; C07K1/061; C07K1/062; C07K17/00; C07K17/02; C07K17/06; C07K17/14; C07K7/00; C07K7/04; C07K7/06; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6809; C12Q1/6813; C12Q1/6816; C12Q1/6834; C12Q1/6837; C12Q1/6869; C12Q1/6874; C40; C40B; C40B80/00; G01N21/17; G01N21/25; G01N21/251; G01N21/253; G01N21/6428; G03; G03F; G03F7/00; G03F7/004; G03F7/0045; G03F7/26; G03F7/265; G03F7/38; G11; G11C; G11C13/00; G11C13/0002; G11C13/0009; G11C13/0014; G11C13/0019; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00279; B01J2219/00306; B01J2219/00313; B01J2219/00315; B01J2219/00351; B01J2219/00427; B01J2219/00432; B01J2219/00434; B01J2219/00436; B01J2219/00457; B01J2219/00459; B01J2219/00468; B01J2219/00475; B01J2219/00497; B01J2219/005; B01J2219/00527; B01J2219/00529; B01J2219/00531; B01J2219/00583; B01J2219/00585; B01J2219/0059; B01J2219/00596; B01J2219/00603; B01J2219/00605; B01J2219/00608; B01J2219/00612; B01J2219/00614; B01J2219/00617; B01J2219/00623; B01J2219/00626; B01J2219/00632; B01J2219/00637; B01J2219/00646; B01J2219/00648; B01J2219/00659; B01J2219/0068; B01J2219/00686; B01J2219/00689; B01J2219/00695; B01J2219/00709; B01J2219/00711; B01J2219/00718; B01J2219/0072; B01J2219/00722; B01J2219/00725; C07B; C07B2200/00; C07B2200/11; C40B40/00; C40B40/04; C40B40/06; C40B40/10; C40B60/00; C40B60/14; G01N15/00; G01N15/10; G01N15/14; G01N15/1429; G01N15/1433; G01N21/6456; G01N21/6458; Y; Y02; Y02P; Y02P20/00; Y02P20/50; Y02P20/55; Y10; Y10S; Y10S435/00; Y10S435/969; Y10S435/973; Y10S436/00; Y10S436/807; Y10S436/809
Claims Analysis
Total Claims: 36
Claim 1
Independent Claim
23 words
A compound comprising the following structure: ##STR25## wherein Y is the C-5' oxygen group of a deoxyribonucleic acid; and R 3 is alkyl.
Claim 2
Dependent Claim
23 words
A compound comprising the following structure: ##STR25## wherein Y is the C-5' oxygen group of a deoxyribonucleic acid; and R 3 is alkyl.
Claim 3
Dependent Claim
10 words
The compound of claim 1, wherein R 3 is methyl.
Claim 4
Dependent Claim
47 words
The compound of claim 1 having the formula: ##STR26## wherein B is selected from the group consisting of adenyl, guanyl, cytidyl, thymidyl, and uridyl; and X is hydrogen, a solid support, an oligonucleotide or an activated phosphorous group selected from the group consisting of phosphotriester and phosphoramidite.
Claim 5
Dependent Claim
11 words
The compound of claim 3, wherein X is a solid support.
Claim 6
Dependent Claim
9 words
The compound of claim 4, wherein B is adenyl.
Claim 7
Dependent Claim
9 words
The compound of claim 4, wherein B is guanyl.
Claim 8
Dependent Claim
9 words
The compound of claim 4, wherein B is cytidyl.
Claim 9
Dependent Claim
9 words
The compound of claim 4, wherein B is thymidyl.
Claim 10
Dependent Claim
9 words
The compound of claim 4, wherein B is uridyl.
Claim 11
Dependent Claim
10 words
The compound of claim 4, wherein R 3 is methyl.
Claim 12
Dependent Claim
9 words
The compound of claim 10, wherein B is adenyl.
Claim 13
Dependent Claim
9 words
The compound of claim 10, wherein B is guanyl.
Claim 14
Dependent Claim
9 words
The compound of claim 10, wherein B is cytidyl.
Claim 15
Dependent Claim
9 words
The compound of claim 10, wherein B is thymidyl.
Claim 16
Dependent Claim
9 words
The compound of claim 10, wherein B is uridyl.
Claim 17
Dependent Claim
10 words
The compound of claim 3, wherein X is an oligonucleotide.
Claim 18
Dependent Claim
10 words
The compound of claim 16, wherein R 3 is methyl.
Claim 19
Dependent Claim
9 words
The compound of claim 17, wherein B is adenyl.
Claim 20
Dependent Claim
9 words
The compound of claim 17, wherein B is guanyl.
Claim 21
Dependent Claim
9 words
The compound of claim 17, wherein B is cytidyl.
Claim 22
Dependent Claim
9 words
The compound of claim 17, wherein B is thymidyl.
Claim 23
Dependent Claim
9 words
The compound of claim 17, wherean B is uridyl.
Claim 24
Dependent Claim
14 words
The compound of claim 16, wherean said oligonucleotide is attached to a synthesis substrate.
Claim 25
Dependent Claim
10 words
The compound of claim 23, wherein R 3 is methyl.
Claim 26
Dependent Claim
9 words
The compound of claim 23, wherein B is adenyl.
Claim 27
Dependent Claim
9 words
The compound of claim 23, wherein B is guanyl.
Claim 28
Dependent Claim
9 words
The compound of claim 23, wherean B is cytidyl.
Claim 29
Dependent Claim
9 words
The compound of claim 23, wherein B is thymidyl.
Claim 30
Dependent Claim
9 words
The compound of claim 23, wherean B is uridyl.
Claim 31
Dependent Claim
17 words
The compound of claim 3, wherein X is selected from the group consisting of phosphatetriester and phosphoramidite.
Claim 32
Dependent Claim
9 words
The compound of claim 30, wherein B is adenyl.
Claim 33
Dependent Claim
9 words
The compound of claim 30, wherein B is guanyl.
Claim 34
Dependent Claim
9 words
The compound of claim 30, wherein B is thymidyl.
Claim 35
Dependent Claim
9 words
The compound of claim 30, wherein B is cytidyl.
Claim 36
Dependent Claim
9 words
The compound of claim 30, wherein B is uridyl.
References Cited (Backward Citations)
US4269933A
Cited
EP0046083A2
Cited
EP0103197A1
Cited
US4516833A
Cited
US4562157A
Cited
US4713326A
Cited
US4704353A
Cited
US5258506A
Cited
WO1986006487A1
Cited
US4833092A
Cited
US4811062A
Cited
US4846552A
Cited
GB2196476A
Cited
US5079600A
Cited
US4981985A
Cited
US4946942A
Cited
WO1989010977A1
Cited
WO1989011548A1
Cited
US4984100A
Cited
WO1990003382A1
Cited
WO1990004652A1
Cited
EP0392546A2
Cited
US5143854A
Cited
US5252743A
Cited
WO1994010128A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-02-05 | 1999-08-07 | 2000-02-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-02-05 | 2003-08-07 | 2004-02-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-02-05 | 2007-08-07 | 2008-02-06 | $7700.00 | Window Closed / Lapsed |