Patent US5266466A successfully refreshed from Google Patents.
US5266466A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of using T7 DNA polymerase to label the 3' end of a DNA molecule
Assignee: Harvard University
•
Filing: 1992-09-04
•
Granted: 1993-11-30
•
Expires: 2012-09-04
Abstract
Abstract This invention relates to T7-type DNA polymerases and methods for using them.
Inventors
- 👤 Stanley Tabor
- 👤 Charles C. Richardson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 10:17
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/26; C12P19/28; C12P19/30; C12P19/34; C12N; C12N9/00; C12N9/10; C12N9/12; C12N9/1241; C12N9/1252; C12Q; C12Q1/00; C12Q1/68; C12Q1/6806; C12Q1/6844; C12Q1/6858; C12Q1/686; C12Q1/6869
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
70 words
A method for labelling the 3' end of a DNA fragment comprising incubating said DNA fragment with T7-type DNA polymerase produced from recombinant DNA, having less than 500 units of exonuclease activity per mg of polymerase, and a labelled deoxynucleotide species under conditions in which said 3' end of said DNA fragment is extended by said polymerase and thereby labelled by addition of said labelled deoxynucleotide to said DNA fragment.
Claim 2
Independent Claim
70 words
A method for labelling the 3' end of a DNA fragment comprising incubating said DNA fragment with T7-type DNA polymerase produced from recombinant DNA, having less than 500 units of exonuclease activity per mg of polymerase, and a labelled deoxynucleotide species under conditions in which said 3' end of said DNA fragment is extended by said polymerase and thereby labelled by addition of said labelled deoxynucleotide to said DNA fragment.
References Cited (Backward Citations)
US3444041A
Cited
US3444042A
Cited
US3661893A
Cited
US4072574A
Cited
US4363877A
Cited
US4363877B1
Cited
US4224408A
Cited
US4331589A
Cited
US4591565A
Cited
US4663283A
Cited
US4555486A
Cited
US4394443A
Cited
US4395486A
Cited
US4563419A
Cited
US4486539A
Cited
US4663290A
Cited
US4483922A
Cited
US4483920A
Cited
US4556643A
Cited
US4521509A
Cited
US4656127A
Cited
US4683196A
Cited
US4729947A
Cited
US4786600A
Cited
US4675283A
Cited
US4767708A
Cited
US4670379A
Cited
US4795700A
Cited
US4683202A
Cited
US4683202B1
Cited
US4707235A
Cited
US4800159A
Cited
EP0258017A2
Cited
US4795699A
Cited
US5145776A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-11-29 | 1997-05-31 | 1997-11-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-11-29 | 2001-05-31 | 2001-11-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-11-29 | 2005-05-31 | 2005-11-30 | $7700.00 | Window Closed / Lapsed |