US4663290A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Production of reverse transcriptase
Assignee: MGI Pharma Inc
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Filing: 1982-01-21
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Granted: 1987-05-05
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Expires: 2002-01-21
Abstract
Abstract Methods are described for the production of the β-subunit of reverse transcriptase using recombinant DNA techniques. Methods and compositions are described for cloning and expressing the gene coding for the β-subunit of reverse transcriptase and characterizing and isolating the gene product. The recombinant plasmids constructed herein contain the necessary signals and controls for efficient expression of the reverse transcriptase gene in bacteria and other microorganisms. The isolated reverse transcriptase gene product is a functional, metabolically stable and biologically active protein.
Inventors
- 👤 John H. Weis
- 👤 John S. Salstrom
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 14:36
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C12; C12N; C12N9/00; C12N9/10; C12N9/12; C12N9/1241; C12N9/1276; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/11011; C12N2740/11022
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
50 words
A recombinant plasmid comprising a first DNA sequence derived from an Avian Sarcoma Virus genome and coding for a protein having reverse transcriptase activity, and a second DNA sequence having expression control elements, said recombinant plasmid being capable of replication and directing expression of said DNA sequence in Escherichia coli.
Claim 2
Dependent Claim
50 words
A recombinant plasmid comprising a first DNA sequence derived from an Avian Sarcoma Virus genome and coding for a protein having reverse transcriptase activity, and a second DNA sequence having expression control elements, said recombinant plasmid being capable of replication and directing expression of said DNA sequence in Escherichia coli.
Claim 3
Dependent Claim
15 words
The recombinant plasmid of claim 1 further comprising the essential portions of the pBR322 replicon.
Claim 4
Dependent Claim
11 words
An Escherichia coli bacterium comprising the recombinant plasmid of claim 1.
Claim 5
Dependent Claim
17 words
An Escherichia coli bacterium of claim 3 and as deposited with the ATCC deposit accession No. 31990.
Claim 6
Dependent Claim
8 words
A recombinant plasmid of claim 2 designated pRT432-1.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-05-04 | 1990-11-03 | 1991-05-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-05-04 | 1994-11-03 | 1995-05-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-05-04 | 1998-11-03 | 1999-05-05 | $7700.00 | Window Closed / Lapsed |