US4224408A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Deoxyribonucleic acid synthesis using binding protein
Assignee: Abbott Laboratories
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Filing: 1978-11-22
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Granted: 1980-09-23
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Expires: 1998-11-22
Abstract
Abstract Described is a method of obtaining complete copying of the entire length of single stranded ribonucleic acid (RNA) into its complementary deoxyribonucleic acid (cDNA) by reverse transcription using binding protein. The method can be used in recombinant DNA research to copy total messenger RNA into DNA.
Inventors
- 👤 Paul P. Hung
- 👤 Shaw-guang Lee
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 05:43
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/26; C12P19/28; C12P19/30; C12P19/34; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1096
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
76 words
A method of reverse transcription of the total length of a single stranded ribonucleic acid (RNA) such as Rous sarcoma virus RNA into its complementary deoxyribonucleic acid (cDNA), said method comprising: incubating Rous sarcoma virus ribonucleic acid in the presence of reverse transcriptase, and nucleic acid binding protein devoid of nuclease activity and isolated from Rous sarcoma virus transformed chicken embryonic fibroblast or untransformed chick embryonic fibroblasts and actinomycin D, and isolating the deoxyribonucleic acid product.
Claim 2
Independent Claim
76 words
A method of reverse transcription of the total length of a single stranded ribonucleic acid (RNA) such as Rous sarcoma virus RNA into its complementary deoxyribonucleic acid (cDNA), said method comprising: incubating Rous sarcoma virus ribonucleic acid in the presence of reverse transcriptase, and nucleic acid binding protein devoid of nuclease activity and isolated from Rous sarcoma virus transformed chicken embryonic fibroblast or untransformed chick embryonic fibroblasts and actinomycin D, and isolating the deoxyribonucleic acid product.
Claim 3
Dependent Claim
21 words
The method of claim 1 wherein the deoxyribonucleic acid product is isolated by gel filtration chromatography and electrophoresis of the product.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-09-23 | 1984-03-24 | 1984-09-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-09-23 | 1988-03-24 | 1988-09-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-09-23 | 1992-03-24 | 1992-09-23 | $7700.00 | Window Closed / Lapsed |