US5002876A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Yeast production of human tumor necrosis factor
Assignee: Research Corp Technologies Inc
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Filing: 1986-09-22
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Granted: 1991-03-26
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Expires: 2006-09-22
Abstract
Abstract Novel DNA constructs comprising yeast regulatory regions plus the structural coding region for human tumor necrosis factor, are disclosed. These novel constructs are incorporated into a variety of linear and circular plasmids. Such plasmids are used for yeast transformation and ultimately for the production of human tumor necrosis factor by yeast.
Inventors
- 👤 Kotikanyadan Sreekrishna
- 👤 Motohiro Fuke
- 👤 Rica H. Potenz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 22:13
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/80; C12N15/81; C12N15/815; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/525; C12N15/67; C12N15/68
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
27 words
An essentially pure culture of a strain of the species Pichia pastoris transformed with a plasmid selected from the group consisting of the plasmids pTNF4 and pTNF6.
Claim 2
Dependent Claim
27 words
An essentially pure culture of a strain of the species Pichia pastoris transformed with a plasmid selected from the group consisting of the plasmids pTNF4 and pTNF6.
Claim 3
Dependent Claim
3 words
The plasmid pTNF4.
Claim 4
Dependent Claim
3 words
The plasmid pTNF4.
Claim 5
Dependent Claim
3 words
The plasmid pTNF6.
Claim 6
Dependent Claim
3 words
The plasmid pTNF6.
Claim 7
Dependent Claim
40 words
A process for preparing human tumor necrosis factor which comprises cultivating a yeast strain transformed with the plasmid of claim 2 under conditions under which the regulatory region induces expression of the polypeptide coding region encoding human tumor necrosis factor.
Claim 8
Dependent Claim
17 words
A process in accordance with claim 4 further comprising isolating and purifying said human tumor necrosis factor.
Claim 9
Dependent Claim
40 words
A process for preparing human tumor necrosis factor which comprises cultivating a yeast strain transformed with the plasmid of claim 3 under conditions under which the regulatory region induces expression of the polypeptide coding region encoding human tumor necrosis factor.
Claim 10
Dependent Claim
17 words
A process in accordance with claim 6 further comprising isolating and purifying said human tumor necrosis factor.
References Cited (Backward Citations)
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Cited
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Cited
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Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-03-25 | 1994-09-24 | 1995-03-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-03-25 | 1998-09-24 | 1999-03-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-03-25 | 2002-09-24 | 2003-03-26 | $7700.00 | Window Closed / Lapsed |