EP0123811B1
Expired / Lapsed
EP
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The use of the gal 1 yeast promoter
Assignee: Oscient Pharmaceuticals Corp
•
Filing: 1984-02-23
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Granted: 1991-06-12
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Expires: 2004-02-23
Abstract
Abstract A DNA-segment containing a GAL 1 promoter linked to a gene outher than the gagactokinase gene for directing the expression of the gene within a yeast cell is new and may be used in a method of expression of a polypeptide in yeast, comprising introducing a GAL 1 promotor in a DNA-segment, said segment being linked to a gene which may be foreign to the yeast genome in a chromosome or vector in such a fashion that said chromosome or vector is replicated and carried by the cell as part of its genetic information and said gene is expressed. The DNA-segment may be contained in a vector, e.g. a plasmid.
Inventors
- 👤 David Botstein
- 👤 Ronald Wayne Davis
- 👤 Gerald Ralph Fink
- 👤 Alison Taunton-Rigby
- 👤 Robert Gentry Knowlton
- 👤 Jen-I Mao
- 👤 Donald Taylor Moir
- 👤 Christopher Godfrey Goff
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 22:58
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6478; C12N9/6481; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C07K14/56; C07K14/575; C07K14/61; C12N15/00; C12N15/09; C12N15/63; C12N15/66; C12N15/79; C12N15/80; C12N15/81; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/94; Y10S435/942; Y10S930/00; Y10S930/01; Y10S930/12; Y10S930/14; Y10S930/142
Claims Analysis
Total Claims: 60
Claim 1
Independent Claim
74 words
A DNA segment containing yeast GAL 1 promoter linked to a gene other than the galactokinase gene for directing the expression of the gene within a yeast cell, said yeast GAL 1 promoter being either a 755 or 820 base pair DNA sequence from the yeast genome which contains signals for transcription of the galactokinase gene into mRNA and subsequent translation of the mRNA or fragments or mutant sequences thereof retaining said promoter function.
Claim 2
Dependent Claim
16 words
A DNA segment as in claim 1, wherein said gene is a bovine growth hormone gene.
Claim 3
Dependent Claim
14 words
A DNA segment as in claim 1, wherein said gene is an interferon gene.
Claim 4
Dependent Claim
14 words
A DNA segment as in claim 1, wherein said gene is a prorennin gene.
Claim 5
Dependent Claim
14 words
A DNA segment as in claim 1, wherein said gene is a pre-prorennin gene.
Claim 6
Dependent Claim
18 words
A DNA segment as in claim 1, wherein said GAL 1 promoter is a 755 base-pair DNA sequence.
Claim 7
Dependent Claim
18 words
A DNA segment as in claim 1, wherein said GAL 1 promoter is a 820 base-pair DNA sequence.
Claim 8
Dependent Claim
21 words
A GAL 1 promoter according to claim 1 linked to a DNA segment for use in expressing a desired heterologous protein.
Claim 9
Dependent Claim
64 words
A method of expression of a polypeptide in yeast, said method comprising introducing a GAL 1 promoter according to claim 1 in a DNA segment, said segment being linked to a gene in a chromosome or vector in such a fashion that said chromosome or vector is replicated and carried by the cell as part of its genetic information and said gene is expressed.
Claim 10
Dependent Claim
15 words
A method as in claim 9, wherein said gene is foreign to the yeast genome.
Claim 11
Dependent Claim
22 words
A method as in claim 9, wherein said polypeptide is bovine growth hormone and said gene is a bovine growth hormone gene.
Claim 12
Dependent Claim
18 words
A method as in claim 9, wherein said polypeptide is interferon and said gene is an interferon gene.
Claim 13
Dependent Claim
18 words
A method as in claim 9, wherein said polypeptide is prorennin and said gene is a prorennin gene.
Claim 14
Dependent Claim
18 words
A method as in claim 9, wherein said polypeptide is pre-prorennin and said gene is a pre-prorennin gene.
Claim 15
Dependent Claim
16 words
A method as in claim 9, wherein said yeast is of the strain Saccharomyces cerevisiae .
Claim 16
Dependent Claim
72 words
A method of obtaining a polypeptide in yeast by the use of a GAL 1 promoter according to claim 1 in a DNA segment linked to a gene foreign to the yeast genome, which DNA segment is incorporated in yeast cells, growing said yeast cells in a medium containing glucose, wherein said yeast cells metabolize said glucose, and permitting said cells to express said polypeptide when galactose is present in the medium.
Claim 17
Dependent Claim
22 words
A method as in claim 16, wherein said polypeptide is bovine growth hormone and said gene is a bovine growth hormone gene.
Claim 18
Dependent Claim
18 words
A method as in claim 16, wherein said polypeptide is interferon and said gene is an interferon gene.
Claim 19
Dependent Claim
18 words
A method as in claim 16, wherein said polypeptide is prorennin and said gene is a prorennin gene.
Claim 20
Dependent Claim
18 words
A method as in claim 16, wherein said polypeptide is pre-prorennin and said gene is a pre-prorennin gene.
Claim 21
Dependent Claim
17 words
Yeast strain as deposited in the American Type Culture Collection under Accession Number 20643, Strain Designation CGY196.
Claim 22
Dependent Claim
17 words
Yeast strain as deposited in the American Type Culture Collection under Accession Number 20661, Strain Designation CGY457.
Claim 23
Dependent Claim
17 words
Yeast strain as deposited in the American Type Culture Collection under Accession Number 20662, Strain Designation CGY461.
Claim 24
Dependent Claim
17 words
Yeast strain as deposited in the American Type Culture Collection under Accession Number 20663, Strain Designation CGY528.
Claim 25
Dependent Claim
52 words
The synthetic DNA sequence P GAL 1 - A₆ C C C C G G A T C T C G A C C - A T G - X, where P GAL1 is a yeast GAL1 promoter according to claim 1 and X is a gene other than the galactokinase gene.
Claim 26
Dependent Claim
57 words
The synthetic DNA sequence P GAL 1 - T T A T T C C T C T A C C G G A T C A A - A T G - X, where P GAL1 is a yeast GAL 1 promoter according to claim 1 and X is a gene other than the galactokinase gene.
Claim 27
Dependent Claim
65 words
The recombinant DNA sequence P GAL 1 - A₆ C C C C G G A T C T C G A C C - A T G - X, where P GAL 1 is the 820 base-pair DNA sequence for the GAL 1 promoter for galactokinase according to claim 7, and X is the DNA sequence for a polypeptide to be expressed in yeast.
Claim 28
Dependent Claim
15 words
The recombinant DNA sequence as in claim 27, wherein said polypeptide is bovine growth hormone.
Claim 29
Dependent Claim
13 words
The recombinant DNA sequence as in claim 27, wherein said polypeptide is interferon.
Claim 30
Dependent Claim
13 words
The recombinant DNA sequence as in claim 27, wherein said polypeptide is prorennin.
Claim 31
Dependent Claim
69 words
The recombinant DNA sequence P GAL1 - T T A T T C C T C T A C C G G A T C A A - A T G - X , where P GAL 1 is the 755 base-pair DNA sequence for the GAL 1 promoter for galactokinase according to claim 6, and X is the DNA sequence for a polypeptide to be expressed in yeast.
Claim 32
Dependent Claim
12 words
The recombinant DNA sequence as in claim 31,wherein said polypeptide is pre-prorennin.
Claim 33
Dependent Claim
37 words
A DNA segment comprising a GAL 1 promoter according to claim 1 derived from the yeast genome that carries the promoter for the major regulated messenger RNA transcript of the galactokinase gene linked to a foreign gene.
Claim 34
Dependent Claim
17 words
A DNA segment as in claim 33, wherein said foreign gene is a bovine growth hormone gene.
Claim 35
Dependent Claim
15 words
A DNA segment as in claim 33, wherein said foreign gene is an interferon gene.
Claim 36
Dependent Claim
15 words
A DNA segment as in claim 33, wherein said foreign gene is a prorennin gene.
Claim 37
Dependent Claim
15 words
A DNA segment as in claim 33, wherein said foreign gene is a pre-prorennin gene.
Claim 38
Dependent Claim
18 words
A DNA segment as in claim 33, wherein said GAL 1 promoter has a 0.82 kilobase DNA sequence.
Claim 39
Dependent Claim
18 words
A DNA segment as in claim 33, wherein said GAL 1 promoter has a 0.755 kilobase DNA sequence.
Claim 40
Dependent Claim
56 words
A plasmid comprised of plasmid YIp5 having modifications comprising: a fragment of the yeast 2µ plasmid containing an initiation site for replication in yeast at the Pvu II site of said plasmid, and a fragment from yeast chromosomal DNA containing a GAL 1 promoter according to claim 1 at the Eco RI site of said plasmid.
Claim 41
Dependent Claim
18 words
A plasmid as in claim 40, having a bovine growth hormone gene linked to said GAL 1 promoter.
Claim 42
Dependent Claim
16 words
A plasmid as in claim 40 having a interferon gene linked to said GAL 1 promoter.
Claim 43
Dependent Claim
16 words
A plasmid as in claim 40 having a prorennin gene linked to said GAL 1 promoter.
Claim 44
Dependent Claim
16 words
A plasmid as in claim 40 having a pre-prorennin gene linked to said GAL 1 promoter.
Claim 45
Dependent Claim
32 words
A plasmid comprised of plasmid YIp5 having modifications comprising a fragment from yeast chromosomal DNA containing a GAL 1 promoter according to claim 1 at the Eco RI site of said plasmid.
Claim 46
Dependent Claim
22 words
A plasmid as in claim 45, wherein said gene for selection in yeast is a DNA segment comprising the Ura 3 gene.
Claim 47
Dependent Claim
18 words
A plasmid as in claim 45 having a bovine growth hormone gene linked to said GAL 1 promoter.
Claim 48
Dependent Claim
16 words
A plasmid as in claim 45 having an interferon gene linked to said GAL 1 promoter.
Claim 49
Dependent Claim
16 words
A plasmid as in claim 45 having a prorennin gene linked to said GAL 1 promoter.
Claim 50
Dependent Claim
16 words
A plasmid as in claim 45 having a pre-prorennin gene linked to said GAL 1 promoter.
Claim 51
Dependent Claim
21 words
The recombinant DNA material obtainable from the yeast strain identified as American Type Culture Collection Accession Number 20643, Strain Designation CGY196.
Claim 52
Dependent Claim
21 words
The recombinant DNA material obtainable from the yeast strain identified as American Type Culture Collection Accession Number 20661, Strain Designation CGY457.
Claim 53
Dependent Claim
21 words
The recombinant DNA material obtainable from the yeast strain identified as American Type Culture Collection Accession Number 20662, Strain Designation CGY461.
Claim 54
Dependent Claim
21 words
The recombinant DNA material obtainable from the yeast strain identified as American Type Culture Collection Accession Number 20663, Strain Designation CGY528.
Claim 55
Dependent Claim
30 words
A vector carrying a GAL 1 promoter according to claim 1 linked to a gene foreign to the yeast genome, said vector being capable of stably transforming yeast and bacteria.
Claim 56
Dependent Claim
14 words
A vector as in claim 55,wherein said gene is a bovine growth hormone gene.
Claim 57
Dependent Claim
12 words
A vector as in claim 55,wherein said gene is an interferon gene.
Claim 58
Dependent Claim
13 words
A vector as in claim 55, wherein said gene is a prorennin gene.
Claim 59
Dependent Claim
13 words
A vector as in claim 55, wherein said gene is a pre-prorennin gene.
Claim 60
Dependent Claim
17 words
A DNA segment as in claim 1, wherein said GAL 1 promoter has the following nucleotide sequence:
References Cited (Backward Citations)
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