US4677064A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Human tumor necrosis factor
Assignee: Novartis Vaccines and Diagnostics Inc
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Filing: 1985-05-02
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Granted: 1987-06-30
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Expires: 2005-05-02
Abstract
Abstract Human Tumor necrosis factor (TNF) has been prepared using recombinant methods. A human promyelocytic leukemia cell line has been induced using an improved induction procedure, and the TNF purified to homogeneity. Methods, vectors, and cells useful in obtaining human TNF in practical amounts are disclosed. Muteins having N-terminal deletions, which muteins have superior biological activity, are also disclosed.
Inventors
- 👤 David F. Mark
- 👤 Alice M. Wang
- 👤 Martha B. Ladner
- 👤 Abla A. Creasey
- 👤 Janelle N. Van Arsdell
- 👤 Leo S. Lin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 10:22
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/525; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/808; Y10S930/00; Y10S930/01; Y10S930/14; Y10S930/144
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
8 words
Human tumor necrosis factor (TNF) which is ∇4-mTNF.
Claim 2
Dependent Claim
8 words
Human tumor necrosis factor (TNF) which is ∇4-mTNF.
Claim 3
Dependent Claim
7 words
A recombinant DNA sequence which encodes ∇4-mTNF.
Claim 4
Dependent Claim
7 words
A recombinant DNA sequence which encodes ∇4-mTNF.
Claim 5
Dependent Claim
10 words
The DNA of claim 2 which is uninterrupted by introns.
Claim 6
Dependent Claim
30 words
A recombinant DNA having a DNA sequence encoding ∇4-mTNF, operably linked to appropriate regulatory control sequences which are capable of effecting the expression of said coding sequences in transformed cells.
Claim 7
Dependent Claim
30 words
A recombinant DNA having a DNA sequence encoding ∇4-mTNF, operably linked to appropriate regulatory control sequences which are capable of effecting the expression of said coding sequences in transformed cells.
Claim 8
Dependent Claim
10 words
Recombinant host cells transformed with the DNA of claim 4.
Claim 9
Dependent Claim
9 words
The cells of claim 5 which are procaryotic cells.
Claim 10
Dependent Claim
9 words
The cells of claim 5 which are mammalian cells.
Claim 11
Dependent Claim
9 words
Human ∇4-mTNF produced by the cells of claim 5.
Claim 12
Independent Claim
49 words
A method for producing human ∇4-mTNF which comprises culturing recombinant host cells wherein a recombinant DNA transformed in said host cells has a DNA sequence encoding ∇4-mTNF, operably linked to appropriate regulatory control sequence which are capable of effecting the expression of said coding sequences in said transformed cells.
Claim 13
Independent Claim
49 words
A method for producing human ∇4-mTNF which comprises culturing recombinant host cells wherein a recombinant DNA transformed in said host cells has a DNA sequence encoding ∇4-mTNF, operably linked to appropriate regulatory control sequence which are capable of effecting the expression of said coding sequences in said transformed cells.
Claim 14
Dependent Claim
26 words
A recombinant vector capable of expression in procaryotic cells which comprises a DNA sequence encoding ∇4-mTNF operably linked to control sequences compatible with said procaryotic cells.
Claim 15
Dependent Claim
26 words
A recombinant vector capable of expression in procaryotic cells which comprises a DNA sequence encoding ∇4-mTNF operably linked to control sequences compatible with said procaryotic cells.
Claim 16
Dependent Claim
10 words
Recombinant procaryotic cells transformed with the vector of claim 10.
Claim 17
Dependent Claim
8 words
The vector of claim 11 which is pAW736.
Claim 18
Dependent Claim
14 words
The recombinant DNA of claim 10 which includes a lambda derived P L promoter.
Claim 19
Dependent Claim
16 words
The recombinant DNA of claim 13 wherein the recombinant DNA includes an N-gene ribosome binding site.
References Cited (Backward Citations)
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Cited
GB2106117A
Cited
US4495282A
Cited
US4447355A
Cited
EP0100641A2
Cited
US4457916A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-06-29 | 1990-12-29 | 1991-06-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-06-29 | 1994-12-29 | 1995-06-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-06-29 | 1998-12-29 | 1999-06-30 | $7700.00 | Window Closed / Lapsed |