EP0123544A2
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EP
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Process for expressing heterologous protein in yeast, expression vehicles and yeast organisms therefor
Assignee: Genentech Inc
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Filing: 1984-04-24
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Granted: —
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Expires: 2004-04-24
Abstract
Abstract The isolation of the yeast α-factor genes is described. The promoter and signal peptide portions are isolated and joined to DNA coding for proteins heterologous to yeast in a plasmid which is used to transform yeast cells. The yeast expresses the heterologous DNA and processes and secretes the heterologous protein.
Inventors
- 👤 Arjun Singh
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-26 05:19
CPC Classifications
C; C12; C12N; C12N15/00; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6478; C12N9/6481; C07; C07K; C07K14/00; C07K14/37; C07K14/39; C07K14/395; C07K14/435; C07K14/52; C07K14/555; C12N15/09; C12N15/63; C12N15/79; C12N15/80; C12N15/81; C12N9/6424; C12N9/6456; C12N9/6459; C12Y; C12Y304/00; C12Y304/21; C12Y304/21069; C07K2319/00; C07K2319/01; C07K2319/02
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
63 words
A process for obtaining a protein heterologous to yeast as a product of yeast expression, which comprises: transforming a yeast organism with a yeast expression vehicle comprising the DNA sequence of the promoter for yeast alpha factor operably connected to a DNA sequence encoding a protein . heterologous to the yeast organism; culturing the transformed organism; and recovering the protein from the culture.
Claim 2
Dependent Claim
63 words
A process for obtaining a protein heterologous to yeast as a product of yeast expression, which comprises: transforming a yeast organism with a yeast expression vehicle comprising the DNA sequence of the promoter for yeast alpha factor operably connected to a DNA sequence encoding a protein . heterologous to the yeast organism; culturing the transformed organism; and recovering the protein from the culture.
Claim 3
Dependent Claim
69 words
A process for obtaining a protein heterologous to yeast as a product of yeast expression, which process comprises: transforming a yeast organism with an expression vehicle comprising the DNA sequence encoding substantially the pre-pro peptide of yeast alpha factor operably connected in translation reading frame to a DNA sequence encoding a mature protein heterologous to the yeast organism; culturing the transformed organism; and recovering the protein from the culture.
Claim 4
Dependent Claim
69 words
A process for obtaining a protein heterologous to yeast as a product of yeast expression, which process comprises: transforming a yeast organism with an expression vehicle comprising the DNA sequence encoding substantially the pre-pro peptide of yeast alpha factor operably connected in translation reading frame to a DNA sequence encoding a mature protein heterologous to the yeast organism; culturing the transformed organism; and recovering the protein from the culture.
Claim 5
Dependent Claim
83 words
A process for obtaining a protein heterologous to yeast as a product of yeast expression, processing and secretion, which process comprises: transforming a yeast organism with an expression vehicle comprising the DNA sequence of the promoter operably linked to substantially the pre-pro peptide sequence of the yeast alpha factor. gene which is operably connected in translation reading frame to a DNA sequence encoding a mature protein heterologous to the yeast organism; culturing the transformed yeast organism; and recovering protein from its supporting medium.
Claim 6
Dependent Claim
83 words
A process for obtaining a protein heterologous to yeast as a product of yeast expression, processing and secretion, which process comprises: transforming a yeast organism with an expression vehicle comprising the DNA sequence of the promoter operably linked to substantially the pre-pro peptide sequence of the yeast alpha factor. gene which is operably connected in translation reading frame to a DNA sequence encoding a mature protein heterologous to the yeast organism; culturing the transformed yeast organism; and recovering protein from its supporting medium.
Claim 7
Dependent Claim
61 words
A process for secreting a protein heterologous to yeast into the supporting medium, which process comprises: transforming a yeast organism with an expression vehicle comprising the DNA sequence encoding substantially the pre-pro peptide of yeast alpha factor, operably connected in translation reading frame to a DNA sequence encoding a mature protein heterologous to the yeast organism; and culturing the transformed organism.
Claim 8
Dependent Claim
61 words
A process for secreting a protein heterologous to yeast into the supporting medium, which process comprises: transforming a yeast organism with an expression vehicle comprising the DNA sequence encoding substantially the pre-pro peptide of yeast alpha factor, operably connected in translation reading frame to a DNA sequence encoding a mature protein heterologous to the yeast organism; and culturing the transformed organism.
Claim 9
Dependent Claim
17 words
A process of Claim 4 wherein said DNA sequences are under the control of alpha factor promoter.
Claim 10
Dependent Claim
31 words
A yeast expression vehicle comprising the DNA sequence of the promoter of the yeast alpha factor gene operably connected to a DNA sequence encoding a protein heterologous to the yeast organism.
Claim 11
Dependent Claim
31 words
A yeast expression vehicle comprising the DNA sequence of the promoter of the yeast alpha factor gene operably connected to a DNA sequence encoding a protein heterologous to the yeast organism.
Claim 12
Dependent Claim
41 words
An expression vehicle of Claim 6 which also includes the DNA sequence encoding substantially the pre-pro peptide of yeast alpha factor operably linked in translation reading frame upstream to the DNA sequence encoding a mature protein heterologous to the yeast organism.
Claim 13
Dependent Claim
37 words
A yeast expression vehicle comprising the DNA sequence encoding substantially the pre-pro peptide of yeast alpha factor gene operably connected in translation reading frame to a DNA sequence encoding a mature protein heterologous to the yeast organism.
Claim 14
Dependent Claim
37 words
A yeast expression vehicle comprising the DNA sequence encoding substantially the pre-pro peptide of yeast alpha factor gene operably connected in translation reading frame to a DNA sequence encoding a mature protein heterologous to the yeast organism.
Claim 15
Dependent Claim
34 words
An expression vehicle of Claims 6 to 8 wherein the DNA encoding heterologous protein s.a.g. encodes for a protein selected from the group consisting of human interferon, bovine interferon, tissue plasminogen activator, and rennin.
Claim 16
Dependent Claim
19 words
An expression vehicle of Claims 6 to 8 wherein the DNA encoding heterologous protein encodes for insulin-like growth factor.
Claim 17
Dependent Claim
16 words
A yeast organism transformed with an expression vehicle of any one of Claims 6 to 10.
Claim 18
Dependent Claim
41 words
A yeast organism capable of producing mature heterologous protein in the supportive medium, as a product of expression with a N-terminus pre-sequence derived from yeast alpha factor DNA, processing of said pre-sequence and secretion of the mature protein into the medium.
Claim 19
Dependent Claim
41 words
A yeast organism capable of producing mature heterologous protein in the supportive medium, as a product of expression with a N-terminus pre-sequence derived from yeast alpha factor DNA, processing of said pre-sequence and secretion of the mature protein into the medium.
Claim 20
Dependent Claim
15 words
The organism of Claim 12 wherein the mature heterologous protein is human insulin-like growth factor.
References Cited (Backward Citations)
No citation records indexed.
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