Patent US4952336A successfully refreshed from Google Patents.
US4952336A
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Fluorescence polymerization immunoassay for amphetamine/methamphetamine
Assignee: Abbott Laboratories
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Filing: 1989-03-10
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Granted: 1990-08-28
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Expires: 2009-03-10
Abstract
Abstract This disclosure relates to a method and reagents for determining amphetamine and methamphetamine in a biological fluid such as urine. In particular, this disclosure relates to a fluorescence polarization immunoassay procedure for determining the presence of amphetamine and methamphetamine in a single assay and to a novel class of tracer compounds employed as reagents in such procedures. The procedure described includes pretreatment of the biological sample to eliminate cross-reactants such as β-hydroxyphenethylamine by preincubating the sample solely with an aqueous periodate solution having a pH from about 4.0 to about 7.5 without adjustment to an alkaline pH.
Inventors
- 👤 Paul J. Brynes
- 👤 Cynthia M. Molina
- 👤 Janis A. Martinus
- 👤 Kenward S. Vaughan
- 👤 Catherine M. Smith
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 15:39
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; G01N33/94; G01N33/946
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
86 words
A tracer reagent useful in a fluorescence polarization immunoassay for determining phenethylamines, said reagent comprising a first tracer having a formula: ##STR5## and a second tracer of the formula: ##STR6## wherein Q is fluorescein or a fluorescein derivative and T is SO 2 , NH, HN(CH 2 ) 3 O, COCH 2 , CO(CH 2 ) 2 , CONH, HN(CH 2 ) 2 or HN(CH 2 ) 2 NHCOCH 2 , said first and second tracers being specifically recognizable by separate antibodies for amphetamine and methamphetamine.
Claim 2
Dependent Claim
86 words
A tracer reagent useful in a fluorescence polarization immunoassay for determining phenethylamines, said reagent comprising a first tracer having a formula: ##STR5## and a second tracer of the formula: ##STR6## wherein Q is fluorescein or a fluorescein derivative and T is SO 2 , NH, HN(CH 2 ) 3 O, COCH 2 , CO(CH 2 ) 2 , CONH, HN(CH 2 ) 2 or HN(CH 2 ) 2 NHCOCH 2 , said first and second tracers being specifically recognizable by separate antibodies for amphetamine and methamphetamine.
Claim 3
Dependent Claim
23 words
A tracer reagent according to claim 1, wherein said first tracer has a formula: ##STR7## and said second tracer has a formula: ##STR8##
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-08-27 | 1994-02-26 | 1994-08-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-08-27 | 1998-02-26 | 1998-08-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-08-27 | 2002-02-26 | 2002-08-28 | $7700.00 | Window Closed / Lapsed |