US3856469A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Interferant removal from amphetamine immunoassay
Assignee: SYVA CORP
•
Filing: 1973-01-02
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Granted: 1974-12-24
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Expires: 1993-01-02
Abstract
Abstract A method for removing Beta -hydroxyamine compounds as an interferant from immunoassays, where like compounds, such as amphetamine, methamphetamine, and the like are to be determined. The sample, for example, urine is treated with aqueous periodate at a mildly basic pH for a time sufficient to remove the Beta hydroxyamine as an interferant, the pH being maintained by an ammonium hydroxide. The sample may then be used in assays for the determination of amphetamine or like compounds without interference from the Beta -hydroxyamine compound.
Inventors
- 👤 R Schneider
- 👤 E Ullman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 19:51
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; G01N33/946; Y; Y10; Y10S; Y10S436/00; Y10S436/803; Y10S436/815; Y10S436/816; Y10S436/825; Y10S436/826
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
54 words
IN AN ASSAY METHOD FOR DETERMINING ARALKYL AMINES, WHEREIN 1-ARALKYL B-HYDROXYAMINES INTERFERE, THE IMPROVEMENT WHICH COMPRISES: TREATING THE SAMPLE TO BE ASSAYED AT A PH GREATER THAN ABOUT 8 WITH AN AMOUNT OF AQUEOUS PERIODATE SOLUTION SUFFICIENT TO REMOVE THE HYDROXYAMINE INTERFERANT WHEREIN SAID PH IS MAINTAINED BY THE PRESENCE OF AN AMMONIUM HYDROXIDE.
Claim 2
Dependent Claim
54 words
IN AN ASSAY METHOD FOR DETERMINING ARALKYL AMINES, WHEREIN 1-ARALKYL B-HYDROXYAMINES INTERFERE, THE IMPROVEMENT WHICH COMPRISES: TREATING THE SAMPLE TO BE ASSAYED AT A PH GREATER THAN ABOUT 8 WITH AN AMOUNT OF AQUEOUS PERIODATE SOLUTION SUFFICIENT TO REMOVE THE HYDROXYAMINE INTERFERANT WHEREIN SAID PH IS MAINTAINED BY THE PRESENCE OF AN AMMONIUM HYDROXIDE.
Claim 3
Dependent Claim
24 words
A method according to claim 1, wherein the concentration of said periodate is in the range of about 10 3 to 10 7 moles/ml.
Claim 4
Dependent Claim
24 words
A method according to claim 1, wherein said periodate is at a concentration in the range of about 10 4 to 10 6 moles/ml.
Claim 5
Dependent Claim
35 words
A method according to claim 1, wherein the concentration of said ammonium hydroxide is in an amount of from 2 to 20 times the concentration of periodate and said ammonium hydroxide is tetraalkyl ammonium hydroxide.
Claim 6
Dependent Claim
63 words
In a method for carrying out immunoassays to detect amphetamine or methamphetamine, the improvement which comprises: treating the sample to be assayed with aqueous periodate solution at a concentration in the range of about 10 4 to 10 6 moles/ml in the presence of an ammonium hydroxide in an amount sufficient to provide a pH in the range of about 8 to 9.
Claim 7
Dependent Claim
63 words
In a method for carrying out immunoassays to detect amphetamine or methamphetamine, the improvement which comprises: treating the sample to be assayed with aqueous periodate solution at a concentration in the range of about 10 4 to 10 6 moles/ml in the presence of an ammonium hydroxide in an amount sufficient to provide a pH in the range of about 8 to 9.
Claim 8
Dependent Claim
14 words
A method according to claim 5, wherein said ammonium hydroxide is tetramethyl ammonium hydroxide.
Claim 9
Dependent Claim
12 words
A method according to claim 5, wherein said ammonium hydroxide is NH4OH.
Claim 10
Dependent Claim
37 words
A method according to claim 5, wherein said treating is carried out at a temperature in the range of about 10* to 40* C and for a time in the range of about 1 to 10 minutes.
Claim 11
Dependent Claim
26 words
An aqueous solution which is 0.05 to 0.5 M in periodate and 0.5 to 2 M in tetraalkylammonium hydroxide of from four to eight carbon atoms.
Claim 12
Dependent Claim
26 words
An aqueous solution which is 0.05 to 0.5 M in periodate and 0.5 to 2 M in tetraalkylammonium hydroxide of from four to eight carbon atoms.
Claim 13
Dependent Claim
24 words
A solution according to claim 9, wherein said tetraalkylammonium hydroxide is tetramethylammonium hydroxide and said periodate ranges from 0.05 to 0.3 M in concentration.
References Cited (Backward Citations)
US3766162A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-12-23 | 1978-06-24 | 1978-12-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-12-23 | 1982-06-24 | 1982-12-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-12-23 | 1986-06-24 | 1986-12-24 | $7700.00 | Window Closed / Lapsed |