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US4943636A
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Certain pyridyl-di-sulfide-alkylenecarbonxylate-ortho-nitro-phenylsulfonic acid esters useful for coupling biological materials
Assignee: Novartis Vaccines and Diagnostics Inc
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Filing: 1987-09-10
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Granted: 1990-07-24
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Expires: 2007-09-10
Abstract
Abstract Coupling of biological materials is disclosed via 4-hydroxy-3-nitrobenzene sulfonic acid sodium salt (HNSA) esters. The novel activated esters are structured so as to react at one end with an amine group of a selected biological or non-biological material, thereby releasing the spectroscopically monitorable HNSA dianion, and at the other end with, typically, a sulfhydryl group of a second material which may or may not be biological. The esters provide for a cross-linking reaction that may be easily controlled and monitored.
Inventors
- 👤 Danute E. Nitecki
- 👤 Lois Aldwin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 09:54
CPC Classifications
C; C07; C07D; C07D213/00; C07D213/02; C07D213/04; C07D213/60; C07D213/62; C07D213/70; C07D211/00; C07D211/04; C07D211/06; C07D211/36; C07D211/54; C07K; C07K1/00; C07K1/003; C07K1/006; C07K1/10
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
98 words
A water-soluble ester useful for coupling biological materials, said ester being of the formula: ##STR27## wherein R is X--S--S-- wherein Q is selected from the group consisting of ##STR28## wherein R 1 and R 2 are independently selected from the group consisting of H, NO 2 and COOH, and Y is a divalent space moiety covalently linking R to the carboxyl carbon of the ester, and selected from the group consisting of acylic saturated linear alkylene having 1-10 carbon atoms, branched alkylene having 1-10 carbon atoms and cycloalkylene having 5 to 8 carbon atoms in their cyclic rings.
Claim 2
Dependent Claim
98 words
A water-soluble ester useful for coupling biological materials, said ester being of the formula: ##STR27## wherein R is X--S--S-- wherein Q is selected from the group consisting of ##STR28## wherein R 1 and R 2 are independently selected from the group consisting of H, NO 2 and COOH, and Y is a divalent space moiety covalently linking R to the carboxyl carbon of the ester, and selected from the group consisting of acylic saturated linear alkylene having 1-10 carbon atoms, branched alkylene having 1-10 carbon atoms and cycloalkylene having 5 to 8 carbon atoms in their cyclic rings.
Claim 3
Dependent Claim
11 words
The water soluble ester of claim 1 wherein R is X--S--S--.
Claim 4
Dependent Claim
17 words
The water soluble ester of claim 4 wherein X is selected from the group consisting of ##STR29##
Claim 5
Dependent Claim
36 words
The water soluble ester of claim 1 wherein Y is selected from alkylene having from 1 to 10 carbon atoms in their chains and cycloalkylene having from 5 to 9 carbon atoms in their cyclic rings.
Claim 6
Dependent Claim
77 words
The water-soluble ester of claim 1 wherein the ester is given by the formula: ##STR30## in which Y is a divalent acrylic hydrocarbon spacer moiety consisting of chain selected from the group consisting of an alkylene having 1 to 10 atoms cycloalkylene having 5 to 8 carbon atoms and X is selected from the group consisting of ##STR31## where R 1 and R 2 are independently selected from the group consisting of --H, --NO 2 and --COOH.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-07-23 | 1994-01-22 | 1994-07-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-07-23 | 1998-01-22 | 1998-07-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-07-23 | 2002-01-22 | 2002-07-24 | $7700.00 | Window Closed / Lapsed |