US4334069A
Expired / Lapsed
US
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Chemiluminescent phthalhydrazide-labeled hapten conjugates
Assignee: Bayer Corp
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Filing: 1978-07-24
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Granted: 1982-06-08
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Expires: 1998-07-24
Abstract
Abstract Chemiluminescent-labeled conjugates of the formula: <IMAGE> wherein one of R1 and R2 is hydrogen and the other is -NR3R4; R3 is hydrogen or straight chain alkyl containing 1-4 carbon atoms and R4 is L(CO-HN-CH2)n wherein n=2-8 and L(CO)- is a hapten bound through an amide bond. The labeled conjugates are useful as reagents in specific binding assays for determining haptens or their specific binding partners in liquid media.
Inventors
- 👤 Robert T. Buckler
- 👤 Hartmut R. Schroeder
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 14:30
CPC Classifications
C; C07; C07D; C07D209/00; C07D209/02; C07D209/44; C07D209/48; C07D237/00; C07D237/26; C07D237/30; C07D237/32; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; Y; Y10; Y10S; Y10S530/00; Y10S530/807
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
54 words
A chemiluminescent-labeled conjugate of the formula: L(CO) (NH)labeling substance wherein L(CO)-- is a hapten of molecular weight greater than 100 bound to said labeling substance through an amide bond, characterized in that said --(NH)labeling substance has the formula: ##STR32## wherein R 3 is hydrogen or straight chain alkyl having 1-4 carbon atoms and n=2-8.
Claim 2
Dependent Claim
54 words
A chemiluminescent-labeled conjugate of the formula: L(CO) (NH)labeling substance wherein L(CO)-- is a hapten of molecular weight greater than 100 bound to said labeling substance through an amide bond, characterized in that said --(NH)labeling substance has the formula: ##STR32## wherein R 3 is hydrogen or straight chain alkyl having 1-4 carbon atoms and n=2-8.
Claim 3
Dependent Claim
11 words
The labeled conjugate of claim 1 wherein said hapten is thyroxine.
Claim 4
Dependent Claim
11 words
The labeled conjugate of claim 1 wherein said hapten is liothyronine.
Claim 5
Dependent Claim
35 words
The labeled conjugate of any one of claims 1, 2 or 3 wherein said --(NH)labeling substance has the formula: ##STR33## wherein R 3 is hydrogen or straight chain alkyl containing 1-4 carbon atoms and n=2-8.
Claim 6
Dependent Claim
8 words
The labeled conjugate of claim 4 wherein n=4.
Claim 7
Dependent Claim
11 words
The labeled conjugate of claim 5 wherein R 3 is ethyl.
Claim 8
Dependent Claim
8 words
The labeled conjugate of claim 4 wherein n=6.
Claim 9
Dependent Claim
11 words
The labeled conjugate of claim 7 wherein R 3 is ethyl.
Claim 10
Dependent Claim
1 words
6-{N-Ethyl-N-[4-(thyroxinylamido)butyl]amino}-2,3-dihydrophthalazine-1,4-dione.
Claim 11
Dependent Claim
1 words
6-{N-Ethyl-N-[4-(thyroxinylamido)butyl]amino}-2,3-dihydrophthalazine-1,4-dione.
Claim 12
Dependent Claim
1 words
6-{N-Ethyl-N-[6-(thyroxinylamido)hexyl]amino}-2,3-dihydrophthalazine-1,4-dione.
Claim 13
Dependent Claim
1 words
6-{N-Ethyl-N-[6-(thyroxinylamido)hexyl]amino}-2,3-dihydrophthalazine-1,4-dione.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-06-07 | 1985-12-07 | 1986-06-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-06-07 | 1989-12-07 | 1990-06-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-06-07 | 1993-12-07 | 1994-06-08 | $7700.00 | Window Closed / Lapsed |