Reagent suitable for enzyme immuno assay

Claim 2

An enzyme-labeled antigen suitable for an enzyme immuno assay having the following formula: ##STR15## wherein X and Y are different and are each an enzyme or antigen.

Claim 3

The enzyme-labeled antigen according to claim 1, which has the following formula: ##STR16## wherein X and Y are the same as defined in claim 1.

Claim 4

The enzyme-labeled antigen according to claim 1, wherein X is an antigen and Y is an enzyme.

Claim 5

The enzyme-labeled antigen according to claim 3, wherein X is insulin and Y is β-D-galactosidase.

Claim 6

The enzyme-labeled antigen according to claim 3, wherein X is triiodothyronine and Y is β-D-galactosidase.

Claim 7

The enzyme-labeled antigen according to claim 3, wherein X is angiotensin I and Y is β-D-galactosidase.

Claim 8

The enzyme-labeled antigen according to claim 1, wherein X is an enzyme and Y is an antigen.

Claim 9

The enzyme-labeled antigen according to claim 1, wherein one of X and Y is a hormone.

Claim 10

The enzyme-labeled antigen according to claim 5, wherein the hormone is a member selected from the group consisting of insulin, angiotensin I and iodothyronines.

Claim 11

The enzyme-labeled antigen according to claim 1, wherein one of X and Y is a hapten.

Claim 12

The enzyme-labeled antigen according to claim 10, wherein the hapten is diphenylpropylamine.

Claim 13

The enzyme-labeled antigen according to claim 1, wherein Y is β-D-galactosidase.

Claim 14

The enzyme-labeled antigen according to claim 1, wherein X is a member selected from the group consisting of peroxidase, glucose oxidase and alkaline phosphatase.

Claim 15

In a method for conducting an enzyme immuno assay which comprises subjecting an enzyme-labeled antigen, an unlabeled antigen and an antibody to a competitive antigen-antibody reaction in a buffered solution, separating the enzyme-labeled antigen bound to the antibody from the free labeled antigen, and determining the amount of the unlabeled antigen by measuring an enzyme activity of either the labeled antigen bound to the antibody or the free labeled antigen, the improvement wherein the enzyme-labeled antigen has the following formula: ##STR17## wherein X and Y are different and are each an enzyme or antigen.

Claim 16

In a method for conducting an enzyme immuno assay which comprises subjecting an enzyme-labeled antigen, an unlabeled antigen and an antibody to a competitive antigen-antibody reaction in a buffered solution, separating the enzyme-labeled antigen bound to the antibody from the free labeled antigen, and determining the amount of the unlabeled antigen by measuring an enzyme activity of either the labeled antigen bound to the antibody or the free labeled antigen, the improvement wherein the enzyme-labeled antigen has the following formula: ##STR17## wherein X and Y are different and are each an enzyme or antigen.

Claim 17

A kit for an enzyme immuno assay which comprises subjecting an enzyme-labeled antigen, an unlabeled antigen and an antibody to a competitive antigen-antibody reaction in a buffered solution, separating the enzyme-labeled antigen bound to the antibody from the free labeled antigen, and determining the amount of the unlabeled antigen by measuring an enzyme activity of either the labeled antigen bound to the antibody or the free labeled antigen, said kit containing the following three components as essential components: Component A: an enzyme-labeled antigen of the formula: ##STR18## wherein X and Y are different and are each an enzyme or antigen, Component B: an antibody against the antigen of component A, and Component C: a substrate for the enzyme of the component A.

Claim 18

A kit for an enzyme immuno assay which comprises subjecting an enzyme-labeled antigen, an unlabeled antigen and an antibody to a competitive antigen-antibody reaction in a buffered solution, separating the enzyme-labeled antigen bound to the antibody from the free labeled antigen, and determining the amount of the unlabeled antigen by measuring an enzyme activity of either the labeled antigen bound to the antibody or the free labeled antigen, said kit containing the following three components as essential components: Component A: an enzyme-labeled antigen of the formula: ##STR18## wherein X and Y are different and are each an enzyme or antigen, Component B: an antibody against the antigen of component A, and Component C: a substrate for the enzyme of the component A.

Claim 19

The kit according to claim 15, wherein the Component A is an enzyme-labeled antigen of the formula: ##STR19##

Claim 20

A process for preparing an enzyme-labeled antigen suitable for an enzyme immuno assay having the following formula: ##STR20## wherein X and Y are different and are each an enzyme or antigen, which comprises reacting a compound of the formula: ##STR21## with an antigen or enzyme containing an amino group but having no thiol group under conditions which produce a bound product of the formula: ##STR22## wherein X is the same as defined above, and reacting the resulting bound product (III) with an enzyme or antigen containing a thiol group under conditions which produce the enzyme labeled antigen.

Claim 21

A process for preparing an enzyme-labeled antigen suitable for an enzyme immuno assay having the following formula: ##STR20## wherein X and Y are different and are each an enzyme or antigen, which comprises reacting a compound of the formula: ##STR21## with an antigen or enzyme containing an amino group but having no thiol group under conditions which produce a bound product of the formula: ##STR22## wherein X is the same as defined above, and reacting the resulting bound product (III) with an enzyme or antigen containing a thiol group under conditions which produce the enzyme labeled antigen.

Claim 22

The process according to claim 17, wherein the compound (I) is reacted with an antigen containing an amino group but containing no thiol group, and the resulting bound product (III) is reacted with an enzyme containing a thiol group.