US4253996A
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Immunochemical conjugates: method and composition
Assignee: Scripps Research Institute
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Filing: 1980-02-13
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Granted: 1981-03-03
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Expires: 2000-02-13
Abstract
Abstract Techniques for preparing stable conjugates of protein-D-GL which can then be isolated in pure form are disclosed, using hen egg ovalbumin (OVA) as a prototype protein, and conjugation and purification methods are described which involve (1) application of a coupling method employing m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS) as the coupling reagent, and (2) introduction of biotin moieties into D-GL molecules to allow application of avidinbiotin system for affinity chromatographic purification of conjugates. These methods are applicable to the preparation of D-GL conjugates of insulin and Ragweed Antigen E and are of general applicability.
Inventors
- 👤 David H. Katz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 21:59
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/35; A61K39/0005; A61K39/0008; A61K2039/60; A61K2039/6093; A61K2039/62; A61K2039/627; Y; Y10; Y10S; Y10S424/00; Y10S424/805; Y10S424/81
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
13 words
Conjugate of D-glutamic acid-D-lysine copolymer with protein wherein the conjugating link is ##STR1##
Claim 2
Dependent Claim
13 words
Conjugate of D-glutamic acid-D-lysine copolymer with protein wherein the conjugating link is ##STR1##
Claim 3
Dependent Claim
13 words
Conjugate of D-glutamic acid-D-lysine copolymer with antigen wherein the conjugating link is ##STR2##
Claim 4
Dependent Claim
13 words
Conjugate of D-glutamic acid-D-lysine copolymer with antigen wherein the conjugating link is ##STR2##
Claim 5
Dependent Claim
13 words
Conjugate of D-glutamic acid-D-lysine copolymer with insulin wherein the conjugating link is ##STR3##
Claim 6
Dependent Claim
13 words
Conjugate of D-glutamic acid-D-lysine copolymer with insulin wherein the conjugating link is ##STR3##
Claim 7
Dependent Claim
15 words
Conjugate of D-glumatic acid-D-lysine copolymer with ragweed antigen E wherein the conjugating link is ##STR4##
Claim 8
Dependent Claim
15 words
Conjugate of D-glumatic acid-D-lysine copolymer with ragweed antigen E wherein the conjugating link is ##STR4##
References Cited (Backward Citations)
US3794630A
Cited
US4191668A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-03-02 | 1984-09-01 | 1985-03-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-03-02 | 1988-09-01 | 1989-03-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-03-02 | 1992-09-01 | 1993-03-03 | $7700.00 | Window Closed / Lapsed |