Patent US4886836A successfully refreshed from Google Patents.
US4886836A
Expired / Lapsed
US
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Activated medium with low non-specific protein adsorption
Assignee: Pall Corp
•
Filing: 1987-06-03
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Granted: 1989-12-12
•
Expires: 2007-06-03
Abstract
Abstract A porous chemically activated medium having a low affinity for peptide group-containing materials is provided comprising a porous polymeric medium having a low affinity for peptide group-containing materials covalently bound to a residue of an activating agent which is capable of reacting with an acceptor molecule.
Inventors
- 👤 Thomas C. Gsell
- 👤 Richard F. Salinaro
- 👤 Peter J. Degen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 11:58
CPC Classifications
B; B01; B01D; B01D67/00; B01D67/0081; B01D67/0093; B01D67/00931; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54393; G01N33/544; G01N33/545; G01N33/68; B01D2323/00; B01D2323/30; B01D2323/38; Y; Y10; Y10S; Y10S436/00; Y10S436/824; Y10S436/825; Y10S436/827; Y10S436/828; Y10S436/901; Y10S436/905; Y10S521/00; Y10S521/901; Y10S521/905
Claims Analysis
Total Claims: 68
Claim 1
Independent Claim
45 words
A porous chemically activated medium comprising: a porous polymeric medium having a low affinity for peptide group-containing materials, as measured by the Immersion Protein Binding Assay, covalently bound to a residue of an activating agent said residue being capable of reacting with an acceptor molecule.
Claim 2
Dependent Claim
45 words
A porous chemically activated medium comprising: a porous polymeric medium having a low affinity for peptide group-containing materials, as measured by the Immersion Protein Binding Assay, covalently bound to a residue of an activating agent said residue being capable of reacting with an acceptor molecule.
Claim 3
Dependent Claim
20 words
The chemically activated medium of claim 1 wherein said porous polymeric medium comprises a material rich in pendant hydroxyl groups.
Claim 4
Dependent Claim
45 words
The chemically activated medium of claim 2 wherein the porous polymeric medium comprises: (a) a porous polymeric substrate, and (b) a surface-modifying polymeric material having a low affinity for peptide group-containing materials formed at and covalently bonded to the surface of said porous polymeric substrate.
Claim 5
Dependent Claim
27 words
The chemically activated medium of claim 3 wherein said polymeric substrate comprises a material having C--H bonds capable of forming radicals under the influence of ionizing radiation.
Claim 6
Dependent Claim
26 words
The chemically activated medium of claim 3 wherein said surface modifying covalently bound polymeric material is formed from a monomer having at least one hydroxyl group.
Claim 7
Dependent Claim
23 words
The chemically activated medium of claim 5 wherein said monomer from which said covalently bound surface-modifying polymeric material comprises an ethylenically unsaturated compound.
Claim 8
Dependent Claim
15 words
The chemically activated medium of claim 3 wherein said porous polymeric substrate comprises a polyamide.
Claim 9
Dependent Claim
13 words
The chemically activated medium of claim 7 wherein said polyamide is nylon 66.
Claim 10
Dependent Claim
16 words
The chemically activated medium of claim 3 wherein said porous polymeric substrate comprises a hydrophilic material.
Claim 11
Dependent Claim
13 words
The chemically activated medium of claim 3 wherein said polymeric substrate is microporous.
Claim 12
Dependent Claim
15 words
The chemically activated medium of claim 1 wherein said peptide group-containing material comprises proteinaceous material.
Claim 13
Dependent Claim
32 words
The chemically activated medium of claim 1 wherein said medium has an adsorption of proteinaceous material measured by the Immersion Protein Binding Assay of no more than 30 micrograms per square centimeter.
Claim 14
Dependent Claim
26 words
The chemically activated medium of claim 5 wherein said monomer from which said surface-modifying covalently bound polymeric material is formed comprises a hydroxyalkyl acrylate or methacrylate.
Claim 15
Dependent Claim
30 words
The chemically activated medium of claim 5 wherein said monomer from which said surface-modifying covalently bound polymeric material is formed is 2-hydroxyethyl acrylate, 2-hydroxyethyl methacrylate, hydroxypropyl acrylate, or hydroxypropyl methacrylate.
Claim 16
Dependent Claim
18 words
The chemically activated medium of claim 5 wherein said monomer is 2-hydroxyprop-1-yl acrylate, 1-hydroxyprop-2-yl acrylate or mixtures thereof.
Claim 17
Dependent Claim
14 words
The chemically activated medium of claim 1 wherein said activating agent comprises a bisazolide.
Claim 18
Dependent Claim
17 words
The chemically activated medium of claim 1 wherein said activating agent comprises a bisazolide of carbonic acid.
Claim 19
Dependent Claim
13 words
The chemically activated medium of claim 1 wherein said activating agent comprises N,N'-carbonyldiimidazole.
Claim 20
Dependent Claim
47 words
The chemically activated medium of claim 3 wherein said porous polymeric substrate comprises nylon 66, the monomer from which said surface-modifying covalently bound polymeric material is formed is hydroxypropyl acrylate and the activating agent of which a residue is bound to the porous polymeric medium is N,N'-carbonyldiimidazole.
Claim 21
Dependent Claim
39 words
The chemically activated medium of claim 2 the porous polymeric medium comprises: (a) a porous polymeric substrate, and (b) a polymer coating having a low affinity for peptide group-containing materials formed at the surface of said porous polymeric substrate.
Claim 22
Dependent Claim
15 words
The chemically activated medium of claim 20 wherein said porous polymeric substrate comprises a polyamide.
Claim 23
Dependent Claim
13 words
The chemically activated medium of claim 21 wherein said polyamide is nylon 66.
Claim 24
Dependent Claim
16 words
The chemically activated medium of claim 20 wherein said porous polymeric substrate comprises a hydrophilic material.
Claim 25
Dependent Claim
35 words
The chemically activated medium of claim 20 wherein said polymeric coating is formed from a polymer having a plurality of hydroxyl groups and a cross-linking agent having functional groups which react with the hydroxyl groups.
Claim 26
Dependent Claim
15 words
The chemically activated medium of claim 24 wherein said cross-linking agent is a polymeric substance.
Claim 27
Dependent Claim
13 words
The chemically activated medium of claim 20 wherein said polymeric substrate is microporous.
Claim 28
Dependent Claim
15 words
The chemically activated medium of claim 24 wherein said hydroxyl group-containing polymer is hydroxypropyl cellulose.
Claim 29
Dependent Claim
20 words
The chemically activated medium of claim 24 wherein said cross-linking agent is a copolymer of acrylic acid and N-methylol acrylamide.
Claim 30
Dependent Claim
13 words
The chemically activated medium of claim 20 wherein said activating agent comprises N,N'-carbonyldiimidazole.
Claim 31
Dependent Claim
50 words
The chemically activated medium of claim 20 wherein said substrate comprises microporous, hydrophilic nylon 66 and said polymer coating is a product formed from the reaction of hydroxypropyl cellulose and a copolymer of acrylic acid and N-methylol acrylamide, and the residue of an activating agent comprises a residue of N,N'-carbonyldiimidazole.
Claim 32
Independent Claim
40 words
A method of producing a chemically activated medium comprising reacting a porous polymeric medium having a low affinity for peptide group-containing materials, as measured by the Immersion Protein Binding Assay, with an activating agent to form a chemically activated medium.
Claim 33
Independent Claim
40 words
A method of producing a chemically activated medium comprising reacting a porous polymeric medium having a low affinity for peptide group-containing materials, as measured by the Immersion Protein Binding Assay, with an activating agent to form a chemically activated medium.
Claim 34
Dependent Claim
55 words
The method of producing a chemically activated medium of claim 31 wherein said porous polymeric medium comprises a material rich in pendant hydroxyl groups including: a porous polymeric substrate, and a surface-modifying polymeric material having a low affinity for peptide group-containing materials formed at and covalently bonded to the surface of said porous polymeric substrate.
Claim 35
Dependent Claim
32 words
The method of producing a porous chemically activated medium of claim 32 wherein said polymeric substrate comprises a material having C--H bonds capable of forming radicals under the influence of ionizing radiation.
Claim 36
Dependent Claim
21 words
The method of producing a porous chemically activated medium according to claim 32 wherein said porous polymeric substrate comprises a polyamide.
Claim 37
Dependent Claim
19 words
The method of producing a porous chemically activated medium according to claim 32 wherein said polyamide is nylon 66.
Claim 38
Dependent Claim
20 words
The method of producing a porous chemically activated medium of claim 32 wherein said monomer comprises an ethylenically unsaturated compound.
Claim 39
Dependent Claim
23 words
The method of producing a porous chemically activated medium of claim 32 wherein said polymeric substrate and said chemically activated medium are microporous.
Claim 40
Dependent Claim
53 words
A porous biologically active medium having a low non-specific affinity for peptide group-containing materials comprising a porous polymeric medium having a low affinity for peptide group-containing materials, as measured by the Immersion Protein Binding Assay, and an acceptor molecule covalently bound to the porous polymeric medium through a residue of an activating agent.
Claim 41
Dependent Claim
53 words
A porous biologically active medium having a low non-specific affinity for peptide group-containing materials comprising a porous polymeric medium having a low affinity for peptide group-containing materials, as measured by the Immersion Protein Binding Assay, and an acceptor molecule covalently bound to the porous polymeric medium through a residue of an activating agent.
Claim 42
Dependent Claim
20 words
The biologically active medium of claim 38 wherein said porous polymeric medium comprises a material rich in pendant hydroxyl groups.
Claim 43
Dependent Claim
43 words
The biologically active medium of claim 38 wherein the porous polymeric medium comprises a porous polymeric substrate, and a surface-modifying polymeric material having a low affinity for peptide group-containing materials formed at and covalently bonded to the surface of said porous polymeric substrate.
Claim 44
Dependent Claim
19 words
The biologically active medium of claim 39 wherein said acceptor molecule is bound to said covalently bound polymeric material.
Claim 45
Dependent Claim
15 words
The biologically active medium of claim 38 wherein said porous polymeric substrate comprises a polyamide.
Claim 46
Dependent Claim
13 words
The biologically active medium of claim 38 wherein said polyamide is nylon 66.
Claim 47
Dependent Claim
16 words
The biologically active medium of claim 40 wherein said biologically active medium is microporous and hydrophilic.
Claim 48
Dependent Claim
15 words
The biologically active medium of claim 40 wherein said acceptor molecule is a proteinaceous material.
Claim 49
Dependent Claim
67 words
The biologically active medium of claim 39 wherein said acceptor molecule comprises a biologically active material which is selected from the group consisting of a monoclonal antibody, a polyclonal antibody, an antigenic substance, a glycoprotein, Protein A, a lectin, a carbohydrate, an enzyme substrate, a cofactor, an inhibitor, a hormone, an IgG class of immunoglobulin, a carrier protein, a receptor, heparin, a coagulation factor, and a histone.
Claim 50
Dependent Claim
42 words
The biologically active medium of claim 39 wherein said peptide group-containing material comprises proteinaceous material and said porous polymeric medium has an adsorption of proteinaceous material measured by the Immersion Protein Binding Assay of not more than 30 micrograms per square centimeter.
Claim 51
Dependent Claim
33 words
The biologically active medium of claim 40 wherein said monomer from which said covalently bound surface-modifying polymeric material is formed is 2-hydroxyethyl acrylate, 2-hydroxyethyl methacrylate, hydroxypropyl acrylate, hydroxypropyl methacrylate and isomeric mixtures thereof.
Claim 52
Dependent Claim
20 words
The biologically active medium of claim 39 wherein said residue of an activating agent is a residue of a bisazolide.
Claim 53
Dependent Claim
23 words
The biologically active medium of claim 39 wherein said residue of an activating agent is a residue of a bisazolide of carbonic acid.
Claim 54
Dependent Claim
19 words
The biologically active medium of claim 39 wherein said residue of an activating agent is a residue of N,N'-carbonyldiimidazole.
Claim 55
Dependent Claim
38 words
The biologically active medium of claim 39 wherein the porous polymeric medium comprises a porous polymeric substrate, and a polymer coating having a low affinity for peptide group-containing materials formed at the surface of said porous polymeric substrate.
Claim 56
Dependent Claim
19 words
The biologically active medium of claim 52 wherein said biologically active material is bound to said porous polymeric substrate.
Claim 57
Dependent Claim
15 words
The biologically active medium of claim 52 wherein said porous polymeric substrate comprises a polyamide.
Claim 58
Dependent Claim
13 words
The biologically active medium of claim 52 wherein said polyamide is nylon 66.
Claim 59
Dependent Claim
35 words
The biologically active medium of claim 52 wherein said polymeric coating is formed from a polymer having a plurality of hydroxyl groups and a cross-linking agent having functional groups which react with the hydroxyl groups.
Claim 60
Dependent Claim
16 words
The biologically active medium of claim 52 wherein said biologically active medium is microporous and hydrophilic.
Claim 61
Dependent Claim
15 words
The biologically active medium of claim 56 wherein said hydroxyl group-containing polymer is hydroxypropyl cellulose.
Claim 62
Dependent Claim
20 words
The biologically active medium of claim 56 wherein said cross-linking agent is a copolymer of acrylic acid and N-methylol acrylamide.
Claim 63
Dependent Claim
20 words
The biologically active medium of claim 52 wherein said residue of an activating agent is a residue of a bisazolide.
Claim 64
Dependent Claim
23 words
The biologically active medium of claim 52 wherein said residue of an activating agent is a residue of a bisazolide of carbonic acid.
Claim 65
Dependent Claim
19 words
The biologically active medium of claim 52 wherein said residue of an activating agent is a residue of N,N'-carbonyldiimidazole.
Claim 66
Dependent Claim
43 words
The biologically active medium of claim 52 wherein said substrate comprises microporous, hydrophilic nylon 66, said polymer coating is a product formed from the reaction of hydroxypropyl cellulose and a copolymer of acrylic acid and N-methylol acrylamide and said activating agent is N,N'-carbonyldiimidazole.
Claim 67
Dependent Claim
16 words
The biologically active medium of claim 52 wherein said biologically active material is a proteinaceous material.
Claim 68
Dependent Claim
62 words
The biologically active medium of claim 52 wherein said biologically active material is selected from the group consisting of a monoclonal antioody, a polyclonal antibody, an antigenic substance, a glycoprotein, Protein A, a lectin, a carbohydrate, an enzyme substrate, a cofactor, an inhibitor, a hormone, an IgG class of immunoglobulin, a carrier protein, a receptor, heparin, a coagulation factor, and a histone.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1992-12-11 | 1993-06-12 | 1993-12-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-12-11 | 1997-06-12 | 1997-12-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-12-11 | 2001-06-12 | 2001-12-12 | $7700.00 | Window Closed / Lapsed |