US4549011A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Modified sheet of material and method of making and using same in connection with biochemical procedures
Assignee: Orgenics Ltd
•
Filing: 1983-09-19
•
Granted: 1985-10-22
•
Expires: 2003-09-19
Abstract
Abstract A sheet for separating and retaining biological molecules. The sheet is activated with a compound for covalently binding a ligand to said sheet, and then coated with ligands having an affinity for the substance of interest. A method of using the sheet for isolating and separating substances of interest and methods for forming the sheet.
Inventors
- 👤 Max Herzberg
- 👤 Falk Fish
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 11:32
CPC Classifications
B; B01; B01D; B01D71/00; B01D71/06; B01D71/08; B01D71/10; B01D15/00; B01D15/08; B01D15/26; B01D15/38; B01D15/3804; B01D61/00; B01D67/00; B01D67/0081; B01D67/0093; B01J; B01J20/00; B01J20/28; B01J20/28014; B01J20/28033; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54353; B01D2311/00; B01D2311/26; B01D2311/2626
Claims Analysis
Total Claims: 46
Claim 1
Independent Claim
8 words
A sheet of material modified by cyanuric chloride.
Claim 2
Dependent Claim
8 words
A sheet of material modified by cyanuric chloride.
Claim 3
Dependent Claim
12 words
The sheet as defined by claim 1 wherein said sheet is cellulosic.
Claim 4
Dependent Claim
15 words
The sheet as defined by claim 1 wherein said sheet is made of plastic material.
Claim 5
Dependent Claim
16 words
The sheet as defined by either of claims 2 or 3 with a ligand bound thereto.
Claim 6
Dependent Claim
19 words
The sheet as defind by claim 4 wherein said ligand is covalently bound to said cyanuric chloride modified sheet.
Claim 7
Dependent Claim
30 words
The sheet as defined by claim 5 wherein said ligand is selected from the group consisting of proteins, polynucleotides, polysaccharides, glycoproteins, lipoproteins, carcinogens, or compounds having a biologically relevant receptor.
Claim 8
Dependent Claim
30 words
The sheet as defined by claim 6 wherein said compound having a biologically relevant receptor is selected from the group consisting of substances capable of affecting the central nervous system.
Claim 9
Dependent Claim
19 words
The sheet as defined by claim 7 wherein said substances capable of affecting the central nervous system are opiates.
Claim 10
Dependent Claim
21 words
The sheet as defined by claim 1 wherein said sheet comprises free hydroxy groups which are reactive with said cyanuric chloride.
Claim 11
Dependent Claim
14 words
The sheet as defined by claim 1 wherein said sheet is a filter paper.
Claim 12
Dependent Claim
14 words
The sheet as defined by claim 1 wherein said sheet is a blotter paper.
Claim 13
Independent Claim
47 words
A method of binding a substance of interest comprising the step of contacting a sheet of material modified by cyanuric chloride having a ligand of said substance of interest bound thereto with said substance of interest to affix said substance of interest onto said sheet of material.
Claim 14
Independent Claim
47 words
A method of binding a substance of interest comprising the step of contacting a sheet of material modified by cyanuric chloride having a ligand of said substance of interest bound thereto with said substance of interest to affix said substance of interest onto said sheet of material.
Claim 15
Dependent Claim
14 words
The method as defined by claim 12 wherein said sheet of material is cellulosic.
Claim 16
Dependent Claim
15 words
The method as defined by claim 12 wherein said sheet is made of plastic material.
Claim 17
Independent Claim
35 words
The method as defined by either of claims 13 or 14 wherein said ligand is selected from the group consisting of proteins, polynucleotides, polysaccharides, glycoproteins, lipoproteins, carcinogens or a compound having a biologically relevant receptor.
Claim 18
Dependent Claim
21 words
The method as defined by claim 15 wherein said ligand consists of a substance capable of affecting the central nervous system.
Claim 19
Dependent Claim
20 words
The method as defined by claim 16 wherein said ligand capable of affecting the central nervous system is an opiate.
Claim 20
Dependent Claim
21 words
The method as defined by claim 12 wherein said sheet comprises free hydroxy groups which are reactive with said cyanuric chloride.
Claim 21
Dependent Claim
14 words
The method as defined by claim 12 wherein said sheet is a filter paper.
Claim 22
Dependent Claim
14 words
The method as defined by claim 12 wherein said sheet is a blotter paper.
Claim 23
Dependent Claim
37 words
The method as defined by claim 12 further comprising contacting said sheet with said substance of interest when said substance of interest is in a gel and wherein said method comprises contacting said sheet with said gel.
Claim 24
Dependent Claim
39 words
The method as defined by claim 12 further comprising contacting said sheet with said substance of interest when said substance of interest is on a separative paper and wherein said method comprises contacting said sheet with said separative paper.
Claim 25
Dependent Claim
23 words
The method as defined by claim 12 further comprising washing said sheet with a solution to remove material not affixed to said sheet.
Claim 26
Dependent Claim
12 words
The method as defined by claim 12 further comprising staining said sheet.
Claim 27
Independent Claim
41 words
A method of forming a modified sheet of material comprising the steps of: (a) modifying said sheet of material by contacting said sheet of material with a liquid solution of cyanuric chloride; and (b) permitting said sheet of material to dry.
Claim 28
Independent Claim
41 words
A method of forming a modified sheet of material comprising the steps of: (a) modifying said sheet of material by contacting said sheet of material with a liquid solution of cyanuric chloride; and (b) permitting said sheet of material to dry.
Claim 29
Dependent Claim
18 words
The method as defined by claim 25 further comprising binding a ligand to said sheet of step (b).
Claim 30
Dependent Claim
12 words
The method as defined by claim 26 wherein said sheet is cellulosic.
Claim 31
Dependent Claim
14 words
The method as defined by claim 26 wherein said sheet is a plastic material.
Claim 32
Dependent Claim
14 words
The method as defined by claim 26 wherein said sheet is a filter paper.
Claim 33
Dependent Claim
30 words
The method as defined by claim 26 wherein said ligand is selected from the group consisting of: proteins, pllynucleotides, polysaccharides, glycoproteins, lipoproteins, carcinogens, or compounds having a biologically relevant receptor.
Claim 34
Dependent Claim
30 words
The method as defined by claim 30 wherein said compounds having a biologically relevant receptor are selected from the group consisting of substances capable of affecting the central nervous system.
Claim 35
Dependent Claim
19 words
The method as defined by claim 31 wherein said substances capable of affecting the central nervous system are opiates.
Claim 36
Dependent Claim
21 words
The method as defined by claim 25 wherein said sheet comprises free hydroxy groups which are reactive with said cyanuric chloride.
Claim 37
Dependent Claim
14 words
The method as defined by claim 25 wherein said sheet is a filter paper.
Claim 38
Dependent Claim
14 words
The method as defined by claim 25 wherein said sheet is a blotter paper.
Claim 39
Dependent Claim
14 words
A sheet of material modified by a composition capble of covalently binding a ligand.
Claim 40
Dependent Claim
14 words
A sheet of material modified by a composition capble of covalently binding a ligand.
Claim 41
Independent Claim
67 words
A method of binding a substance of interest to a sheet of material comprising the steps of: (a) contacting the sheet of material with a composition capable of covalently binding a ligand to said sheet; (b) contacting said sheet with a ligand solution to bind said ligand thereto; (c) contacting said sheet having said ligand bound thereto with said substance of interest to bind said substance thereto.
Claim 42
Independent Claim
67 words
A method of binding a substance of interest to a sheet of material comprising the steps of: (a) contacting the sheet of material with a composition capable of covalently binding a ligand to said sheet; (b) contacting said sheet with a ligand solution to bind said ligand thereto; (c) contacting said sheet having said ligand bound thereto with said substance of interest to bind said substance thereto.
Claim 43
Dependent Claim
23 words
The method as defined by claim 37 further comprising washing said sheet with a solution to remove material not affixed to said sheet.
Claim 44
Dependent Claim
12 words
The method as defined by claim 37 further comprising staining said sheet.
Claim 45
Independent Claim
50 words
A method of forming a modified sheet of material comprising the steps of: (a) modifying said sheet of material by contacting said sheet of material with a liquid solution of a composition capable of covalently binding a liquid to said sheet; and (b) permitting said sheet of material to dry.
Claim 46
Independent Claim
50 words
A method of forming a modified sheet of material comprising the steps of: (a) modifying said sheet of material by contacting said sheet of material with a liquid solution of a composition capable of covalently binding a liquid to said sheet; and (b) permitting said sheet of material to dry.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-10-21 | 1989-04-22 | 1989-10-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-10-21 | 1993-04-22 | 1993-10-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-10-21 | 1997-04-22 | 1997-10-22 | $7700.00 | Window Closed / Lapsed |