Method of increasing natural killer cell population of cancer patients

Claim 1

An in vivo method of increasing the Natural Killer cell population in the blood of a cancer patient, said method comprising the steps of: injecting a biologic into the bloodstream of said cancer patient, and allowing said biologic to operate therein for a period of time to increase said Natural Killer cell population, said biologic being a glycoprotein and having an apparent molecular weight of above about 100,000 and being derived from the blood of an animal injected with a virus, wherein the aminal's blood containing the biologic is fractionated to separate potentially interfering substances from the biologic, said virus being a feline panleukopenia or canine origin parvo virus and said animal being other than a feline or canine which is a nonpermissive host for the virus, said virus-injected animal being allowed to react to the presence of the virus such that 50 microliters of the animal's serum, when added to an in vitro human white blood cell culture containing 2-4×10 5 white blood cells and followed by three days inclubation at 37° C. under a 5% CO 2 /95% air atmostphere, will give rise to at least about a 50% increase in T-helper and Natural Killer cells in the biologic-supplemented cell culture as compared with an otherwise identical in vitro cell culture having added thereto 50 microliters of serum from a normal animal of the same species as said injected animal, such rise in T-helper and Natural Killer cells being detected by staining with fluorescent-labeled monoclonal antibodies against T-Helper and Natural Killer cells.