Biologic and method of preparing same

Claim 1

A method of producing a glycosylated immunomodulating and serum cholesterol and triglyceride level decreasing protein biologic having an apparent molecular weight of above about 100,000, comprising the steps of: injecting a feline panleukopenia or canine origin parvovirus into an animal other than a feline or canine, and which is a non-permissive host for the virus; permitting said injected animal to react to the presence of said virus for a period of time of at least one month for developing in the animal's blood serum said biologic such that 50 microliters of the animal's serum, when added to an in vitro human white blood cell culture containing 2-4×10 5 white blood cells and followed by three days incubation at 37° C. under a 5% CO 2 /95% air atmosphere, will give rise to at least about a 50% increase in T-Helper and Natural Killer cells in the biologic-supplemented cell culture as compared with an otherwise identical and identically cultured in vitro cell culture having added thereto 50 microliters of serum from a normal animal of the same species as said injected animal, such rise in T-Helper and Natural Killer cells being detected by staining with fluorescent-labelled monoclonal antibodies against T-Helper and Natural Killer cells; and recovering blood from said injected animal containing said biologic, and fractionating the recovered blood to separate potentially interfering substances from the biologic.

Claim 2

A method of producing a glycosylated immunomodulating and serum cholesterol and triglyceride level decreasing protein biologic having an apparent molecular weight of above about 100,000, comprising the steps of: injecting a feline panleukopenia or canine origin parvovirus into an animal other than a feline or canine, and which is a non-permissive host for the virus; permitting said injected animal to react to the presence of said virus for a period of time of at least one month for developing in the animal's blood serum said biologic such that 50 microliters of the animal's serum, when added to an in vitro human white blood cell culture containing 2-4×10 5 white blood cells and followed by three days incubation at 37° C. under a 5% CO 2 /95% air atmosphere, will give rise to at least about a 50% increase in T-Helper and Natural Killer cells in the biologic-supplemented cell culture as compared with an otherwise identical and identically cultured in vitro cell culture having added thereto 50 microliters of serum from a normal animal of the same species as said injected animal, such rise in T-Helper and Natural Killer cells being detected by staining with fluorescent-labelled monoclonal antibodies against T-Helper and Natural Killer cells; and recovering blood from said injected animal containing said biologic, and fractionating the recovered blood to separate potentially interfering substances from the biologic.