US4824775A
Expired / Lapsed
US
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Cells labeled with multiple Fluorophores bound to a nucleic acid carrier
Assignee: MILES INC. A CORP. OF INDIANA
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Filing: 1985-01-03
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Granted: 1989-04-25
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Expires: 2005-01-03
Abstract
Abstract In passing labeled cells through a cell sorter, the improvement which comprises employing a labeled cell comprising a cell, an antibody specific to and bound to such cell, a nucleic acid fragment joined to said antibody, and a plurality of labels on said nucleic acid fragment. Because of the presence of multiple labels, the sensitivity of the separation of labeled cells is increased.
Inventors
- 👤 Nanibhushan Dattagupta
- 👤 Michael E. Kamarck
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 12:29
CPC Classifications
C; C07; C07H; C07H21/00; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56966; G01N33/56972; G01N33/58; G01N33/582
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
57 words
A labeled cell consisting essentially of a cell, an antibody specific to and bound to such cell, a nucleic acid fragment covalently coupled to said antibody, and a plurality of fluorophores on said nucleic acid fragment, wherein said fluorophores are coupled to said nucleic acid fragment via a nucleic acid specific intercalator carrying a primary amine residue.
Claim 2
Dependent Claim
57 words
A labeled cell consisting essentially of a cell, an antibody specific to and bound to such cell, a nucleic acid fragment covalently coupled to said antibody, and a plurality of fluorophores on said nucleic acid fragment, wherein said fluorophores are coupled to said nucleic acid fragment via a nucleic acid specific intercalator carrying a primary amine residue.
Claim 3
Dependent Claim
16 words
A labeled cell according to claim 1, wherein said fragment is specifically bound to said antibody.
Claim 4
Dependent Claim
24 words
A labeled cell according to claim 2, wherein said fragment is covalently coupled to a protein which protein is specifically bound to the antibody.
Claim 5
Dependent Claim
54 words
A process for producing a labeled cell according to claim 1 consisting essentially of coupling multiple fluorophore labels to a nucleic acid fragment via a nucleic acid specific intercalator carrying a primary amine residue, covalently coupling said labeled fragment to an antibody specific to a particular cell, and binding said antibody to said cell.
Claim 6
Dependent Claim
37 words
In a method of passing labeled cells through a cell sorter, detecting the labeled cells and sorting out the cells on the basis of their labels, the improvement which comprises employed labeled cells according to claim 1.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-04-24 | 1992-10-24 | 1993-04-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-04-24 | 1996-10-24 | 1997-04-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-04-24 | 2000-10-24 | 2001-04-25 | $7700.00 | Window Closed / Lapsed |