EP0128332A1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Assay method utilizing polynucleotide sequences
Assignee: Enzo Biochem Inc
•
Filing: 1984-05-04
•
Granted: —
•
Expires: 2004-05-04
Abstract
Abstract A method of detecting in a sample an analyte (A) having a molecularly recognizable portion thereon, which comprises: providing (B) a molecular bridging entity having thereon: (i) a portion capable of recognizing the molecularly recognizable portion on the analyte; and (ii) a portion comprising a polynucleotide sequence; and (C) a signalling entity having thereon: (i) a polynucleotide portion capable of annealing to the polynucleotide portion of the bridging entity, thereby to form a stable polynucleotide hybrid, and (ii) a signal generating portion; forming a complex comprising: (1) the analyte (A) complexed through its molecularly recognizable portion to (2) the recognizing portion of the entity (B); the entity (B) being complexed through the polynucleotide portion thereon to (3) the polynucleotide portion of the signalling entity; and detecting a signal by means of the signal generating portion present in the complex.
Inventors
- 👤 Robert G. Pergolizzi
- 👤 Jannis G. Stavrianopoulos
- 👤 Elazar Rabbani
- 👤 Dean L. Engelhardt
- 👤 Stan Kline
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 13:36
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6876; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
140 words
A method of detecting in a sample an analyte (A) having a molecularly recognizable portion thereon, which comprises: providing a molecular bridging entity (B) having thereon: (i) a portion capable of recognizing said molecularly recognizable portion on said analyte; and (ii) a portion comprising a polynucleotide sequence; and (C) a signalling entity having thereon: (i) a polynucleotide portion capable of annealing to said polynucleotide portion of said bridging entity, thereby to form a stable polynucleotide hybrid, and (ii) a signal generating portion; forming a complex comprising: (1) said analyte (A) complexed through said molecularly recognizable portion to (2) said recognizing portion of said entity (B); said entity (B) being complexed through said polynucleotide portion thereon to (3) said polynucleotide portion of said signalling entity (C); and detecting a signal by means of said signal generating portion present in said complex.
Claim 2
Independent Claim
140 words
A method of detecting in a sample an analyte (A) having a molecularly recognizable portion thereon, which comprises: providing a molecular bridging entity (B) having thereon: (i) a portion capable of recognizing said molecularly recognizable portion on said analyte; and (ii) a portion comprising a polynucleotide sequence; and (C) a signalling entity having thereon: (i) a polynucleotide portion capable of annealing to said polynucleotide portion of said bridging entity, thereby to form a stable polynucleotide hybrid, and (ii) a signal generating portion; forming a complex comprising: (1) said analyte (A) complexed through said molecularly recognizable portion to (2) said recognizing portion of said entity (B); said entity (B) being complexed through said polynucleotide portion thereon to (3) said polynucleotide portion of said signalling entity (C); and detecting a signal by means of said signal generating portion present in said complex.
Claim 3
Dependent Claim
15 words
The method of Claim 1 wherein said molecularly recognizable portion on said analyte is proteinaceous.
Claim 4
Dependent Claim
16 words
The method of Claim 1 wherein the molecularly recognizable portion on said analyte comprises nucleic acid.
Claim 5
Dependent Claim
16 words
The method of Claim 1 wherein the molecularly recognizable portion on said analyte comprises a saccharide.
Claim 6
Dependent Claim
163 words
5 . A method of detecting in a sample an analyte (A) having a molecularly recognizable portion thereon, which comprises: providing a molecular bridging entity (B) having thereon: (i) a portion capable of recognizing said molecularly recognizable portion on said analyte (A), and (ii) a portion comprising a recognizable modifier, and a signalling entity (C) having thereon: (i) a receptor for said recognizable modifier capable of attachment or binding to said recognizable modifier of said bridging entity (B) thereby to form a stable complex, and (ii) a signal generating portion, and forming a complex comprising: (1) said analyte (A) attached through said molecularly recognizable portion thereon to (2) said portion of said molecular bridging entity (B) capable of recognizing said molecularly recognizable portion of analyte (A), said molecular bridging entity (B) being complexed via said recognizable modifier thereon to (3) said signalling entity (C) via said receptor thereon capable of attachment to or binding to said recognizable modifier of said bridging entity (B).
Claim 7
Dependent Claim
163 words
5 . A method of detecting in a sample an analyte (A) having a molecularly recognizable portion thereon, which comprises: providing a molecular bridging entity (B) having thereon: (i) a portion capable of recognizing said molecularly recognizable portion on said analyte (A), and (ii) a portion comprising a recognizable modifier, and a signalling entity (C) having thereon: (i) a receptor for said recognizable modifier capable of attachment or binding to said recognizable modifier of said bridging entity (B) thereby to form a stable complex, and (ii) a signal generating portion, and forming a complex comprising: (1) said analyte (A) attached through said molecularly recognizable portion thereon to (2) said portion of said molecular bridging entity (B) capable of recognizing said molecularly recognizable portion of analyte (A), said molecular bridging entity (B) being complexed via said recognizable modifier thereon to (3) said signalling entity (C) via said receptor thereon capable of attachment to or binding to said recognizable modifier of said bridging entity (B).
Claim 8
Dependent Claim
47 words
6 . A stable detectable complex comprising a target analyte, a modified first receptor attached thereto via said receptor portion, the modifying portion thereof being attached to a second receptor for said modifying portion of said first receptor, said second receptor having attached thereto a singalling entity.
Claim 9
Dependent Claim
47 words
6 . A stable detectable complex comprising a target analyte, a modified first receptor attached thereto via said receptor portion, the modifying portion thereof being attached to a second receptor for said modifying portion of said first receptor, said second receptor having attached thereto a singalling entity.
Claim 10
Dependent Claim
10 words
7 . A polynucleotide sequence covalently attached to an antibody.
Claim 11
Dependent Claim
10 words
7 . A polynucleotide sequence covalently attached to an antibody.
Claim 12
Dependent Claim
10 words
The sequence of Claim 72 wherein said antibody is monoclonal.
Claim 13
Dependent Claim
8 words
A polynucleotide sequence covalently attached to a lectin.
Claim 14
Dependent Claim
8 words
A polynucleotide sequence covalently attached to a lectin.
Claim 15
Dependent Claim
14 words
A polynucleotide sequence covalently attached to a saccharide having up to 20 saccharide units.
Claim 16
Dependent Claim
14 words
A polynucleotide sequence covalently attached to a saccharide having up to 20 saccharide units.
Claim 17
Dependent Claim
7 words
A polynucleotide sequence covalently attached to receptor.
Claim 18
Dependent Claim
7 words
A polynucleotide sequence covalently attached to receptor.
Claim 19
Dependent Claim
8 words
A polynucleotide sequence covalently attached to a hormone.
Claim 20
Dependent Claim
8 words
A polynucleotide sequence covalently attached to a hormone.
Claim 21
Dependent Claim
42 words
A DNA molecule carrying a polynucleotide portion which comprises a sequence selected from the group consisting of poly dGT, poly dAC, poly dCT, poly dAt, poly dGC, poly dGA, poly dG, poly dC, poly dT, poly dA, and a repeating low-complexity polynucleotide.
Claim 22
Dependent Claim
42 words
A DNA molecule carrying a polynucleotide portion which comprises a sequence selected from the group consisting of poly dGT, poly dAC, poly dCT, poly dAt, poly dGC, poly dGA, poly dG, poly dC, poly dT, poly dA, and a repeating low-complexity polynucleotide.
Claim 23
Dependent Claim
34 words
14, An entity capable of detecting a protein receptor comprising: (a) a protein capable of binding said protein receptor,and (b) a polynucleotide attached to said protein that is capable of binding said protein receptor.
Claim 24
Dependent Claim
34 words
14, An entity capable of detecting a protein receptor comprising: (a) a protein capable of binding said protein receptor,and (b) a polynucleotide attached to said protein that is capable of binding said protein receptor.
Claim 25
Dependent Claim
18 words
15 . The entity of Claim 14 which further comprises a signal generating portion attached to said polynucleotide.
Claim 26
Dependent Claim
110 words
A kit useful for the detection of an analyte (A) having a molecularly recognizable portion thereon, comprising: I) a carrier being compartmentalized to receive in close confinement therein one or more container means; II) a first container means containing a molecular bridging entity (B) having thereon: (i) a portion capable of recognizing said molecularly recognizble portion on said analyte (A); and (ii) a portion comprising a polynucleotide sequence; and (III) a second container means containing a signalling entity (C) having thereon: (i) a polynucleotide portion capable of annealing to said polynucleotide portion of said bridging entity (B) thereby to form a stable polynucleotide hybrid; and (ii) a signal generating portion.
Claim 27
Dependent Claim
110 words
A kit useful for the detection of an analyte (A) having a molecularly recognizable portion thereon, comprising: I) a carrier being compartmentalized to receive in close confinement therein one or more container means; II) a first container means containing a molecular bridging entity (B) having thereon: (i) a portion capable of recognizing said molecularly recognizble portion on said analyte (A); and (ii) a portion comprising a polynucleotide sequence; and (III) a second container means containing a signalling entity (C) having thereon: (i) a polynucleotide portion capable of annealing to said polynucleotide portion of said bridging entity (B) thereby to form a stable polynucleotide hybrid; and (ii) a signal generating portion.
References Cited (Backward Citations)
US4358535A
Cited
US4358535B1
Cited
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.