US4469796A
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Assaying methods involving biospecific affinity reactions
Assignee: Phadia AB
•
Filing: 1981-05-05
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Granted: 1984-09-04
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Expires: 2001-05-05
Abstract
Abstract An improvement in assaying methods involving biospecific affinity reactions, in which there are used from 2 to 4 reactants, one of which, reactant (I), is labelled with at least one analytically indicatable atom or group and is soluble in the aqueous liquid in which the biospecific affinity reaction is carried out, the reactants forming, by means of biospecific reactions, a conjugate in which labelled reactant (I) is incorporated; and in which assaying methods the analytically indicatable atom or group is assayed in the conjugate and/or in labelled reactant (I), which is not bound to the conjugate. The conjugate that has been formed or labelled reactant (I) not bound to the conjugate is bound covalently to an insoluble carrier or to an insolubilizable carrier, which latter carrier is made insoluble after the covalent binding has been carried out, whereafter the assay of the analytically indicatable atom or group is carried out.
Inventors
- 👤 Rolf E. A. V. Axen
- 👤 Sven O. H. Oscarsson
- 👤 Per H. G. Ponterius
- 👤 Jan P. E. Carlsson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 16:55
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/537; G01N33/538; Y; Y10; Y10S; Y10S436/00; Y10S436/824; Y10S436/827; Y10S436/828
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
221 words
In assaying methods involving biospecific affinity reactions, in which there are used from 2 to 4 reactants, one of which, reactant (I), is labelled with at least one analytically indicatable atom or group and is soluble in the aqueous liquid in which the biospecific affinity reaction is carried out, the reactants forming, by means of biospecific affinity reactions, a conjugate in which labelled reactant (I) is incorporated, and in which assaying methods the analytically indicatable atom or group is assayed in the conjugate and/or in labelled reactant (I) not bound to the conjugate, the improvement which comprises (a) binding, after the formation of the conjugate, the formed conjugate or labelled reactant (I) not bound to the conjugate convalently to an insoluble carrier or to an insolubilizable carrier, which latter is made insoluble after the covalent binding has been carried out, said carrier and said unlabelled reactant incorporated in the conjugate each exhibiting a respective type of reactive groups being of the kind capable of reacting with one another to form a covalent bond between the carrier and said reactant, and none of the reactive groups being present in any other of the reactants incorporated in the conjugate and the unlabelled reactant not competing with labelled reactant (I), and (b) then carrying out the assay of the analytically indicatable atom or group.
Claim 2
Dependent Claim
221 words
In assaying methods involving biospecific affinity reactions, in which there are used from 2 to 4 reactants, one of which, reactant (I), is labelled with at least one analytically indicatable atom or group and is soluble in the aqueous liquid in which the biospecific affinity reaction is carried out, the reactants forming, by means of biospecific affinity reactions, a conjugate in which labelled reactant (I) is incorporated, and in which assaying methods the analytically indicatable atom or group is assayed in the conjugate and/or in labelled reactant (I) not bound to the conjugate, the improvement which comprises (a) binding, after the formation of the conjugate, the formed conjugate or labelled reactant (I) not bound to the conjugate convalently to an insoluble carrier or to an insolubilizable carrier, which latter is made insoluble after the covalent binding has been carried out, said carrier and said unlabelled reactant incorporated in the conjugate each exhibiting a respective type of reactive groups being of the kind capable of reacting with one another to form a covalent bond between the carrier and said reactant, and none of the reactive groups being present in any other of the reactants incorporated in the conjugate and the unlabelled reactant not competing with labelled reactant (I), and (b) then carrying out the assay of the analytically indicatable atom or group.
Claim 3
Dependent Claim
73 words
A method as claimed in claim 1, wherein there is used as said carrier one which exhibits pyridyl disulphide or SH-groups and as said unlabelled reactant one which is incorporated in the conjugate and does not compete with labelled reactant (I) and which exhibits SH-groups when the carrier exhibits pyridyl disulphide groups or pyridyl disulphide groups when the carrier exhibits SH-groups, which groups can react with each other in a thiol-disulphide exchange reaction.
Claim 4
Dependent Claim
67 words
A method as claimed in claim 1 wherein a soluble polymer containing pyridyl disulphide groups is used as the carrier, part of said groups being reduced to SH-groups after the conjugate or labelled reactant (I) not bound to the conjugate has been covalently bound to the carrier, thereby polymerizing the carrier molecules to an insoluble polymer by a reaction between formed SH-groups and residual pyridyl disulphide groups.
Claim 5
Dependent Claim
193 words
In assaying methods involving biospecific affinity reactions, in which there are used from 2 to 4 reactants, one of which, reactant (I), is labelled with at least one analytically indicatable atom or group and is soluble in the aqueous liquid in which the biospecific affinity reaction is carried out, the reactants forming, by means of biospecific affinity reactions, a conjugate in which labelled reactant (I) is incorporated, and in which assaying methods the analytically indicatable atom or group is assayed in the conjugate and/or in labelled reactant (I) not bound to the conjugate, the improvement which comprises (a) binding, after the formation of the conjugate, the labelled reactant (I) not bound to the conjugate covalently to an insoluble carrier exhibiting a molecule network of such mesh size that labelled reactant (I) not bound to the conjugate and the conjugate differ considerably in their ability to penetrate the network, the labelled reactant (I) and network of the carrier exhibiting reactive groups capable of reacting with each other to form a covalent bond between the labelled reactant (I) and the carrier, and (b) then carrying out the assay of the analytically incatable atom or group.
Claim 6
Dependent Claim
193 words
In assaying methods involving biospecific affinity reactions, in which there are used from 2 to 4 reactants, one of which, reactant (I), is labelled with at least one analytically indicatable atom or group and is soluble in the aqueous liquid in which the biospecific affinity reaction is carried out, the reactants forming, by means of biospecific affinity reactions, a conjugate in which labelled reactant (I) is incorporated, and in which assaying methods the analytically indicatable atom or group is assayed in the conjugate and/or in labelled reactant (I) not bound to the conjugate, the improvement which comprises (a) binding, after the formation of the conjugate, the labelled reactant (I) not bound to the conjugate covalently to an insoluble carrier exhibiting a molecule network of such mesh size that labelled reactant (I) not bound to the conjugate and the conjugate differ considerably in their ability to penetrate the network, the labelled reactant (I) and network of the carrier exhibiting reactive groups capable of reacting with each other to form a covalent bond between the labelled reactant (I) and the carrier, and (b) then carrying out the assay of the analytically incatable atom or group.
References Cited (Backward Citations)
US3839153A
Cited
US4180383A
Cited
US4149003A
Cited
US4175073A
Cited
US4231999A
Cited
US4232119A
Cited
US4145406A
Cited
US4139604A
Cited
GB2040935A
Cited
US4272506A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-09-04 | 1988-03-05 | 1988-09-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-09-04 | 1992-03-05 | 1992-09-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-09-04 | 1996-03-05 | 1996-09-04 | $7700.00 | Window Closed / Lapsed |