US3839153A
Expired / Lapsed
US
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Process for the detection and determination of specific binding proteins and their corresponding bindable substances
Assignee: Akzona Inc
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Filing: 1971-12-10
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Granted: 1974-10-01
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Expires: 1991-12-10
Abstract
Abstract The invention relates to a process for the detection and determination of a component of the reaction between a specific binding protein and the corresponding bindable substance contacting a fluid containing one of these components with a conjugate of the bindable substance with an enzyme and of antibodies against the specific binding protein which latter component is brought in an insoluble form, if necessary, in combination with the specific binding protein, and determining the activity of the liquid or solid phase of the resulting reaction mixture.
Inventors
- 👤 A Schuurs
- 👤 Weemen B Van
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 14:06
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/743; G01N33/53; G01N33/536; G01N33/537; G01N33/539; G01N33/541; Y; Y10; Y10S; Y10S435/00; Y10S435/964; Y10S435/971; Y10S435/975; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/815; Y10S436/817; Y10S436/818
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
129 words
PROCESS FOR THE DETECTION AND DETERMINATION OF A BINDABLESUBSTANCE SELECTED FROM THE GROUP CONSISTING OF AN ANTIGEN AND A HAPTEN, COMPRISING THE STEPS OF: A. PROVIDING A GIVEN AQUNTITY OF A CONJUGATE OF SAID BINDABLE SUBSTANCE WITH AN ENZYME; B. PROVIDING A CORRESPONDING GIVEN QUANTITY OF AN ANTIBODY AGAINST SAID BINDABLE SUBSTANCE; C. ADMIXING A SAMPLE OF A FLUID CONTAINING THE BINDABLE SUBSTANCE TO BE DETERMINED WITH THE REACTANTS OF STEPS(A) AND (B) TO FORM A REACTION MIXTURE AND ALLOWING THE REACTION TO GO TO COMPLETION; D. SEPARATING THE RESULTING MIXTURE INTO A LIQUID PHASE AND A SOLID PHASE BY ADDING AN INSOLUBLIZED ANTIBODY AGAINST THE ANTIBODY OF STEP (B); AND E. DETERMINING THE QUANTITY OF THE BINDABLE SUBSTANCE FORM THE MEASURE OF ENZYME ACTIVITY OF EITHER SEPARATED PHASE.
Claim 2
Dependent Claim
129 words
PROCESS FOR THE DETECTION AND DETERMINATION OF A BINDABLESUBSTANCE SELECTED FROM THE GROUP CONSISTING OF AN ANTIGEN AND A HAPTEN, COMPRISING THE STEPS OF: A. PROVIDING A GIVEN AQUNTITY OF A CONJUGATE OF SAID BINDABLE SUBSTANCE WITH AN ENZYME; B. PROVIDING A CORRESPONDING GIVEN QUANTITY OF AN ANTIBODY AGAINST SAID BINDABLE SUBSTANCE; C. ADMIXING A SAMPLE OF A FLUID CONTAINING THE BINDABLE SUBSTANCE TO BE DETERMINED WITH THE REACTANTS OF STEPS(A) AND (B) TO FORM A REACTION MIXTURE AND ALLOWING THE REACTION TO GO TO COMPLETION; D. SEPARATING THE RESULTING MIXTURE INTO A LIQUID PHASE AND A SOLID PHASE BY ADDING AN INSOLUBLIZED ANTIBODY AGAINST THE ANTIBODY OF STEP (B); AND E. DETERMINING THE QUANTITY OF THE BINDABLE SUBSTANCE FORM THE MEASURE OF ENZYME ACTIVITY OF EITHER SEPARATED PHASE.
Claim 3
Dependent Claim
30 words
The process of claim 1 in which the bindable substance is determined by first adding thereto the antibody, then the enzyme conjugate, and finally the insoluble antibody against the antibody.
Claim 4
Dependent Claim
125 words
Process for the detection and determination of an antibody in a fluid sample containing the same utilizing the reaction between said antibody and a bindable substance selected from the group consisting of an antigen and a hapten having an affinity therefor, comprising the steps of: a. providing a given quantity of a conjugate of said bindable substance and an enzyme; b. providing a given quantity of an insolubilized antibody against the antibody to be determined; c. admixing said given quantities of steps (a) and (b) with said sample, and allowing the components to react; d. separating the reaction mixture into a liquid phase and a solid phase; and e. determining the quantity of the antibody from the measure of enzyme activity of either separated phase.
Claim 5
Dependent Claim
125 words
Process for the detection and determination of an antibody in a fluid sample containing the same utilizing the reaction between said antibody and a bindable substance selected from the group consisting of an antigen and a hapten having an affinity therefor, comprising the steps of: a. providing a given quantity of a conjugate of said bindable substance and an enzyme; b. providing a given quantity of an insolubilized antibody against the antibody to be determined; c. admixing said given quantities of steps (a) and (b) with said sample, and allowing the components to react; d. separating the reaction mixture into a liquid phase and a solid phase; and e. determining the quantity of the antibody from the measure of enzyme activity of either separated phase.
Claim 6
Dependent Claim
93 words
A test pack for the detection and determination of a bindable substance selected from the group consisting of an antigen and a hapten in a fluid sample, comprising: a. a known amount of a conjugate of said bindable substance with an enzyme; b. a corresponding known amount of an antibody against said antigen or hapten; c. a known amount of an insolubilized antibody against said antibody; d. a stabilizer; and e. a substrate for the determination of the enzyme activity and thus of the quantity of the antigen or hapten to be determined.
Claim 7
Dependent Claim
93 words
A test pack for the detection and determination of a bindable substance selected from the group consisting of an antigen and a hapten in a fluid sample, comprising: a. a known amount of a conjugate of said bindable substance with an enzyme; b. a corresponding known amount of an antibody against said antigen or hapten; c. a known amount of an insolubilized antibody against said antibody; d. a stabilizer; and e. a substrate for the determination of the enzyme activity and thus of the quantity of the antigen or hapten to be determined.
Claim 8
Dependent Claim
84 words
A test pack for the detection and determination of an antibody in a fluid sample, utilizing the reaction between said antibody and an antigen or hapten to said antibody, comprising: a. a known amount of a conjugate of the antigen or hapten with an enzyme; b. a corresponding known amount of an insolubilized antibody against the antibody to be determined; c. a stabilizer; and d. a substrate for the determination of the enzyme activity and thus of the quantity of antibody to be determined.
Claim 9
Dependent Claim
84 words
A test pack for the detection and determination of an antibody in a fluid sample, utilizing the reaction between said antibody and an antigen or hapten to said antibody, comprising: a. a known amount of a conjugate of the antigen or hapten with an enzyme; b. a corresponding known amount of an insolubilized antibody against the antibody to be determined; c. a stabilizer; and d. a substrate for the determination of the enzyme activity and thus of the quantity of antibody to be determined.
Claim 10
Dependent Claim
111 words
A test pack for the determination of pregnancy by the detection and determination of human chorionic gonadotropin in a sample of urine, utilizing the reaction between human chorionic gonadotropin and an antibody therefor, comprising, in separate lyophilized layers: a. a predetermined amount of a conjugate of human chorionic gonadotropin and an enzyme; b. a predetermined amount of an antibody against human chorionic gonadotropin; c. a predetermined amount of an antibody against the human chorionic gonadotropin antibody, in insolubilized form; d. a stabilizer; and e. a substrate for the determination of the enzyme activity and thus of the quantity of human chorionic gonadotropin enzyme conjugate and human chorionic gonadotropin to be determined.
Claim 11
Dependent Claim
111 words
A test pack for the determination of pregnancy by the detection and determination of human chorionic gonadotropin in a sample of urine, utilizing the reaction between human chorionic gonadotropin and an antibody therefor, comprising, in separate lyophilized layers: a. a predetermined amount of a conjugate of human chorionic gonadotropin and an enzyme; b. a predetermined amount of an antibody against human chorionic gonadotropin; c. a predetermined amount of an antibody against the human chorionic gonadotropin antibody, in insolubilized form; d. a stabilizer; and e. a substrate for the determination of the enzyme activity and thus of the quantity of human chorionic gonadotropin enzyme conjugate and human chorionic gonadotropin to be determined.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-09-30 | 1978-04-01 | 1978-10-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-09-30 | 1982-04-01 | 1982-10-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-09-30 | 1986-04-01 | 1986-10-01 | $7700.00 | Window Closed / Lapsed |