US4520110A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Fluorescent immunoassay employing a phycobiliprotein labeled ligand or receptor
Assignee: Leland Stanford Junior University
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Filing: 1982-12-30
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Granted: 1985-05-28
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Expires: 2002-12-30
Abstract
Abstract Sensitive detection techniques and compositions for such techniques employing fluorescent proteins having bilin prosthetic groups as labels i.e. phycobiliprotein. The bilin containing proteins can be conjugated to ligands or receptors for use in systems involving ligand-receptor binding for the analysis, detection or separation of ligands and receptors. Particularly, one or more of the bilin containing proteins may be used as labels in conjuction with each other or other fluorescers for defining subsets of naturally occurring aggregations e.g. cells.
Inventors
- 👤 Lubert Stryer
- 👤 Alexander N. Glazer
- 👤 Vernon T. Oi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 18:49
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S530/00; Y10S530/82; Y10S530/825
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
82 words
In a fluorescent immunoassay employing as a reagent a fluorescent compound conjugated to a member of a specific binding pair where said pair consists of ligand and receptor, and said immunoassay is for the determination of a member, the binding of the conjugate to a member being indicative of the presence of said member, the improvement which comprises employing in said assay a fluorescent compound comprising a phycobiliprotein conjugated to a member of a specific binding pair consisting of ligand and receptor.
Claim 2
Dependent Claim
82 words
In a fluorescent immunoassay employing as a reagent a fluorescent compound conjugated to a member of a specific binding pair where said pair consists of ligand and receptor, and said immunoassay is for the determination of a member, the binding of the conjugate to a member being indicative of the presence of said member, the improvement which comprises employing in said assay a fluorescent compound comprising a phycobiliprotein conjugated to a member of a specific binding pair consisting of ligand and receptor.
Claim 3
Dependent Claim
57 words
In a fluorescent method for detecting cells having a first specific determinant site, employing for fluorescent labeling a fluorescent reagent comprising a fluorescer and a member of a first specific binding pair, the improvement which comprises employing a fluorescent compound comprising a phycobiliprotein conjugated to a member of a specific binding pair consisting of ligand and receptor.
Claim 4
Dependent Claim
57 words
In a fluorescent method for detecting cells having a first specific determinant site, employing for fluorescent labeling a fluorescent reagent comprising a fluorescer and a member of a first specific binding pair, the improvement which comprises employing a fluorescent compound comprising a phycobiliprotein conjugated to a member of a specific binding pair consisting of ligand and receptor.
Claim 5
Dependent Claim
24 words
In a method according to claim 2, wherein a second fluorescent reagent is employed having different absorption and emission characteristics from said fluorescent compound.
Claim 6
Dependent Claim
83 words
In a method for detecting the presence of a determinant site or a receptor, by employing a fluorescent reagent having a fluorescer bound to a member of a specific binding pair, wherein the binding of said fluorescent reagent to said determinant site or receptor is determined as indicative of the presence of said determinant site or said receptor, the improvement which comprises, employing a fluorescent compound comprising a phycobiliprotein conjugated to a member of a specific binding pair consisting of ligand and receptor.
Claim 7
Dependent Claim
83 words
In a method for detecting the presence of a determinant site or a receptor, by employing a fluorescent reagent having a fluorescer bound to a member of a specific binding pair, wherein the binding of said fluorescent reagent to said determinant site or receptor is determined as indicative of the presence of said determinant site or said receptor, the improvement which comprises, employing a fluorescent compound comprising a phycobiliprotein conjugated to a member of a specific binding pair consisting of ligand and receptor.
References Cited (Backward Citations)
US4193983A
Cited
US4302536A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-05-27 | 1988-11-26 | 1989-05-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-05-27 | 1992-11-26 | 1993-05-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-05-27 | 1996-11-26 | 1997-05-28 | $7700.00 | Window Closed / Lapsed |