Bacteriophages as recognition and identification agents

Claim 1

A method for preparing a bacteriophage binding through a head segment to molecular or cellular materials, comprising the steps of: (a) mixing the bacteriophage, having both head and tail segments, with bacteria; (b) irrading the mixture with ultraviolet light to effect mutation of the bacteriophage; (c) nultiplying the mutant bacteriophage in the presence of bacteria binding through a tail segment to molecular of cellular material; (d) purifying the population of nutant bacteriophage; (e) binding the pruified mutant bacteriophage to the cellular material or to the molecular material; and (f) multiplying the bound mutant bacteriophage in bacteria.

Claim 2

A method for preparing a bacteriophage binding through a head segment to molecular or cellular materials, comprising the steps of: (a) mixing the bacteriophage, having both head and tail segments, with bacteria; (b) irrading the mixture with ultraviolet light to effect mutation of the bacteriophage; (c) nultiplying the mutant bacteriophage in the presence of bacteria binding through a tail segment to molecular of cellular material; (d) purifying the population of nutant bacteriophage; (e) binding the pruified mutant bacteriophage to the cellular material or to the molecular material; and (f) multiplying the bound mutant bacteriophage in bacteria.

Claim 6

A method for preparing a selected bacteriophage binding through a tail segment to molecular or cellular materials, comprising the steps of: (a) injecting molecular or cellular material into an animal host for the preparation of antibodies thereto; (b) recovering and purifying said antibodies; (c) coupling the purified antibodies to a solid phase; (d) binding the coupled antibodies with bacteria characterized as being resistant to a selected bacteriophage; (e) growing and harvesting the bacteria bound to said antibodies; (f) irradiating selected bacteriophage binding through a tail sesgment to molecular of cellular material with ultraviolet light; (g) separately irradiating a strain of bacteria, sensitive to the selected bacteriophage, with ultraviolet light; (h) mixing the irradiated bacteriophage of step (f) with the irradiated bacteria of step (g); (i) mixing approximately equal portions of the products of steps (e) and (h) under conditions for the development of plaques, whereby mutant bacteriophages are generated and yield clear plaques; and (j) recovering the mutant bacteriophage from the clear plaques and multiplying it in the bacteria of step (e), whereby there is obtained a mutant bacteriophage capable of binding to said molecular or cellular materials.

Claim 7

A method for preparing a selected bacteriophage binding through a tail segment to molecular or cellular materials, comprising the steps of: (a) injecting molecular or cellular material into an animal host for the preparation of antibodies thereto; (b) recovering and purifying said antibodies; (c) coupling the purified antibodies to a solid phase; (d) binding the coupled antibodies with bacteria characterized as being resistant to a selected bacteriophage; (e) growing and harvesting the bacteria bound to said antibodies; (f) irradiating selected bacteriophage binding through a tail sesgment to molecular of cellular material with ultraviolet light; (g) separately irradiating a strain of bacteria, sensitive to the selected bacteriophage, with ultraviolet light; (h) mixing the irradiated bacteriophage of step (f) with the irradiated bacteria of step (g); (i) mixing approximately equal portions of the products of steps (e) and (h) under conditions for the development of plaques, whereby mutant bacteriophages are generated and yield clear plaques; and (j) recovering the mutant bacteriophage from the clear plaques and multiplying it in the bacteria of step (e), whereby there is obtained a mutant bacteriophage capable of binding to said molecular or cellular materials.