US4298689A
Expired / Lapsed
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Gonorrhea diagnostic test
Assignee: Research Corp
•
Filing: 1978-09-25
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Granted: 1981-11-03
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Expires: 1998-09-25
Abstract
Abstract Method of diagnosing Neisseria gonorrhoeae infections utilizing plant lectins which have an affinity for N-acetylglucosamine.
Inventors
- 👤 Ronald J. Doyle
- 👤 Kenneth F. Keller
- 👤 Robert L. Schaefer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 18:51
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/12; Y; Y10; Y10S; Y10S436/00; Y10S436/826; Y10S436/827
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
93 words
A method for detecting the presence of Neisseria gonorrhoeae microorganisms which comprises contacting, at a pH of from 4 to 10, a sample suspected of containing a Neisseria gonorrhoeae microorganism with a plant lectin, the said lectin being characterized as one which reacts specifically with N-acetylglucosamine and testing resulting mixture for the presence of a reaction product formed by reaction between the lectin and N-acetylglucosamine on the cell surface of said Neisseria gonorrhoeae microorganism, wherein the presence of such reaction product is a presumptive indication of the presence of a Neisseria gonorrhoeae microorganism.
Claim 2
Independent Claim
93 words
A method for detecting the presence of Neisseria gonorrhoeae microorganisms which comprises contacting, at a pH of from 4 to 10, a sample suspected of containing a Neisseria gonorrhoeae microorganism with a plant lectin, the said lectin being characterized as one which reacts specifically with N-acetylglucosamine and testing resulting mixture for the presence of a reaction product formed by reaction between the lectin and N-acetylglucosamine on the cell surface of said Neisseria gonorrhoeae microorganism, wherein the presence of such reaction product is a presumptive indication of the presence of a Neisseria gonorrhoeae microorganism.
Claim 3
Dependent Claim
23 words
A method as in claim 1 wherein the sample is an exudate from a human suspected of an infection of Neisseria gonorrhoeae microorganisms.
Claim 4
Dependent Claim
29 words
A method as in claim 1 wherein the lectin is tagged with a detectable label selected from the group consisting of fluorescein dye labels, isotape labels and enzyme labels.
Claim 5
Dependent Claim
18 words
A method as in claim 3 wherein the lectin is wheat germ lectin tagged with a fluorescein dye.
Claim 6
Dependent Claim
23 words
A method as in claim 3 wherein the lectin is one obtained from Bandeiraea simplicifolia and that is tagged with a fluorescein dye.
Claim 7
Dependent Claim
23 words
A method as in claim 3 wherein the lectin is one obtained from Ulex europeus and that is tagged with a fluorescein dye.
Claim 8
Independent Claim
106 words
A method for detecting the presence of Neisseria gonorrhoeae microorganisms in a gram negative, oxidase positive culture medium which comprises contacting a suspension of microorganisms from a gram negative, oxidase positive culture medium in an aqueous media at a pH of from 4 to 10 with a plant lectin, the said lectin being characterized as one which reacts specifically with N-acetylglucosamine to effect agglutination caused by reaction between the lectin and N-acetylglucosamine on the cell surface of a Neisseria gonorrhoeae microorganism, and detecting the presence of such agglutination, wherein the presence of such agglutination is a presumptive indication of the presence of a Neisseria gonorrhoeae microorganism.
Claim 9
Independent Claim
106 words
A method for detecting the presence of Neisseria gonorrhoeae microorganisms in a gram negative, oxidase positive culture medium which comprises contacting a suspension of microorganisms from a gram negative, oxidase positive culture medium in an aqueous media at a pH of from 4 to 10 with a plant lectin, the said lectin being characterized as one which reacts specifically with N-acetylglucosamine to effect agglutination caused by reaction between the lectin and N-acetylglucosamine on the cell surface of a Neisseria gonorrhoeae microorganism, and detecting the presence of such agglutination, wherein the presence of such agglutination is a presumptive indication of the presence of a Neisseria gonorrhoeae microorganism.
Claim 10
Dependent Claim
26 words
A method as in claim 7 wherein the culture is generated from a specimen obtained from a human suspected of an infection by Neisseria gonorrhoeae microorganisms.
Claim 11
Dependent Claim
13 words
A method as in claim 8 wherein the lectin is wheat germ lectin.
Claim 12
Dependent Claim
14 words
A method as in claim 8 wherein the lectin is obtained from Ulex europeus.
Claim 13
Dependent Claim
14 words
A method as in claim 8 wherein the lectin is obtained from Bandeiraea simplicifolia.
Claim 14
Dependent Claim
12 words
A method as in claim 8 wherein the agglutination is detected macroscopically.
Claim 15
Dependent Claim
14 words
A method as in claim 12 wherein the lectin is obtained from Bandeiraea simplicifolia.
Claim 16
Dependent Claim
13 words
A method as in claim 12 wherein the lectin is wheat germ lectin.
Claim 17
Dependent Claim
14 words
A method as in claim 12 wherein the lectin is obtained from Ulex europeus.
Claim 18
Dependent Claim
13 words
A method as in claim 7 wherein the lectin is wheat germ lectin.
Claim 19
Dependent Claim
14 words
A method as in claim 7 wherein the lectin is obtained from Bandeiraea simplicifolia.
Claim 20
Dependent Claim
14 words
A method as in claim 7 wherein the lectin is obtained from Ulex europeus.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-11-02 | 1985-05-04 | 1985-11-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-11-02 | 1989-05-04 | 1989-11-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-11-02 | 1993-05-04 | 1993-11-03 | $7700.00 | Window Closed / Lapsed |