Patent US4778767A successfully refreshed from Google Patents.
US4778767A
Expired / Lapsed
US
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Solid phase immunoassay using immunoreagents immobilized on inert synthetic resin surfaces
Assignee: Akzo NV
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Filing: 1984-12-17
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Granted: 1988-10-18
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Expires: 2004-12-17
Abstract
Abstract A solid phase immunoassay comprises the steps of (a) immobilizing an immunoreagent on the surface of a carrier comprised of an inert synthetic resin selected from the group consisting of polyimides and polyfluorinated synthetic resins, (b) contacting the immunoreagent with a complementary immunoreagent whereby an immunocomplex immobilized on said carrier is formed, (c) quantitating the immobilized immunocomplex. An element useful in conducting this solid phase immunoassay is prepared by a process of treating the surface of an article comprised of a synthetic polymer selected from the group consisting of polyimides and polyfluorinated synthetic resins to make it adsorptive of an immunoreagent which comprises the steps of (a) thoroughly rinsing the surface with a water-miscible organic solvent, (b) thoroughly rinsing the surface with water.
Inventors
- 👤 Jan C. Hummelen
- 👤 Theo Luider
- 👤 Hans Wynberg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 16:23
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; Y; Y10; Y10S; Y10S436/00; Y10S436/823; Y10S436/824
Claims Analysis
Total Claims: 45
Claim 1
Independent Claim
53 words
A solid phase immunoassay comprising the steps of (a) immobilizing an immunoreagent on the surface of a carrier comprised of an inert synthetic resin selected from the group consisting of polyimides, (b) contacting said immunoreagent with a complementary immunoreagent whereby an immunocomplex immobilized on said carrier is formed, (c) quantitating said immobilized immunocomplex.
Claim 2
Dependent Claim
53 words
A solid phase immunoassay comprising the steps of (a) immobilizing an immunoreagent on the surface of a carrier comprised of an inert synthetic resin selected from the group consisting of polyimides, (b) contacting said immunoreagent with a complementary immunoreagent whereby an immunocomplex immobilized on said carrier is formed, (c) quantitating said immobilized immunocomplex.
Claim 3
Dependent Claim
16 words
The immunoassay of claim 1 wherein said polyimide is a polymer of pyromellitic acid and bis(4-aminophenyl)oxide.
Claim 4
Dependent Claim
11 words
The immunoassay of claim 1 wherein said immunoreagent is a protein.
Claim 5
Dependent Claim
11 words
The immunoassay of claim 1 wherein said immunoreagent is an antibody.
Claim 6
Dependent Claim
11 words
The immunoassay of claim 1 wherein said immunoreagent is an antigen.
Claim 7
Dependent Claim
32 words
An element for use in a solid phase immunoassay comprising an immunoreagent immobilized on the surface of a carrier comprised of an inert synthetic resin selected from the group consisting of polyimides.
Claim 8
Dependent Claim
32 words
An element for use in a solid phase immunoassay comprising an immunoreagent immobilized on the surface of a carrier comprised of an inert synthetic resin selected from the group consisting of polyimides.
Claim 9
Dependent Claim
16 words
The element of claim 6 wherein said polyimide is a polymer of pyromellitic acid and bis(4-aminophenyl)oxide.
Claim 10
Dependent Claim
11 words
The element of claim 6 wherein said immunoreagent is a protein.
Claim 11
Dependent Claim
11 words
The element of claim 6 wherein said immunoreagent is an antibody.
Claim 12
Dependent Claim
11 words
The element of claim 6 wherein said immunoreagent is an antigen.
Claim 13
Dependent Claim
53 words
The immunoassay of claim 1 wherein the surface of the carrier is treated to make it receptive to adsorption of an immunoreagent prior to immobilizing an immunoreagent thereon by the process comprising the steps of (a) thoroughly rinsing the surface with a water-miscible organic solvent, and (b) thoroughly rinsing the surface with water.
Claim 14
Dependent Claim
16 words
The immunoassay of claim 11 wherein said solvent is a C 1 -C 4 aliphatic alcohol.
Claim 15
Dependent Claim
10 words
The immunoassay of claim 12 wherein said alcohol is ethanol.
Claim 16
Dependent Claim
24 words
The immunoassay of claim 11 wherein said solvent rinsing step is carried out at a temperature equal to the boiling point of the solvent.
Claim 17
Dependent Claim
25 words
The immunoassay of claim 11 wherein said solvent rinsing step is carried out for a period of from about one-half hour to about four days.
Claim 18
Dependent Claim
18 words
The immunoassay of claim 15 wherein said solvent rinsing step is carried out for at least one day.
Claim 19
Dependent Claim
23 words
The immunoassay of claim 11 wherein said water rinsing step is carried out at a temperature equal to the boiling point of water.
Claim 20
Dependent Claim
18 words
The immunoassay of claim 11 wherein the surface of the carrier is dried after the water rinsing step.
Claim 21
Dependent Claim
19 words
The immunoassay of claim 18 wherein said drying step is carried out at a temperature greater than 200° C.
Claim 22
Dependent Claim
19 words
The immunoassay of claim 19 wherein said drying step is carried out at a temperature of about 300° C.
Claim 23
Dependent Claim
18 words
The immunoassay of claim 11 wherein the treated surface is contacted with an aqueous solution containing an immunoreagent.
Claim 24
Dependent Claim
161 words
A solid phase thermochemiluminescent immunoassay comprising the steps of (a) thoroughly rinsing the surface of a carrier comprised of an inert synthetic resin selected from the group consisting of polyfluorinated synthetic resins with a water-miscible organic solvent, (b) thoroughly rinsing the surface of the carrier with water, (c) contacting the surface of the carrier with an aqueous solution of a first immunoreagent for a sufficient time to immobilize at least a portion of the first immunoreagent on said surface, (d) contacting said immobilized first immunoreagent with a complementary immunoreagent whereby an immunocomplex immobilized on said carrier is formed, (e) contacting the surface of the carrier having the immobilized immunocomplex thereon with an immunoreagent that is immunoreactive with either the complementary immunoreagent or the first immunoreagent and has a thermochemiluminescent label to form a detectable labelled immunocomplex immobilized on said carrier, (f) heating said carrier to a temperature at which the thermochemiluminescent label becomes luminescent, and (g) detecting said immobilized thermochemiluminescent immunocomplex.
Claim 25
Dependent Claim
161 words
A solid phase thermochemiluminescent immunoassay comprising the steps of (a) thoroughly rinsing the surface of a carrier comprised of an inert synthetic resin selected from the group consisting of polyfluorinated synthetic resins with a water-miscible organic solvent, (b) thoroughly rinsing the surface of the carrier with water, (c) contacting the surface of the carrier with an aqueous solution of a first immunoreagent for a sufficient time to immobilize at least a portion of the first immunoreagent on said surface, (d) contacting said immobilized first immunoreagent with a complementary immunoreagent whereby an immunocomplex immobilized on said carrier is formed, (e) contacting the surface of the carrier having the immobilized immunocomplex thereon with an immunoreagent that is immunoreactive with either the complementary immunoreagent or the first immunoreagent and has a thermochemiluminescent label to form a detectable labelled immunocomplex immobilized on said carrier, (f) heating said carrier to a temperature at which the thermochemiluminescent label becomes luminescent, and (g) detecting said immobilized thermochemiluminescent immunocomplex.
Claim 26
Dependent Claim
19 words
The immunoassay of claim 22 wherein said polyfluorinated synthetic resin is a polymer of a polyfluorinated ethylenically unsaturated hydrocarbon.
Claim 27
Dependent Claim
12 words
The immunoassay of claim 23 wherein said polyfluorinated synthetic resin is polytetrafluoroethylene.
Claim 28
Dependent Claim
12 words
The immunoassay of claim 22 wherein said first immunoreagent is a protein.
Claim 29
Dependent Claim
12 words
The immunoassay of claim 22 wherein said first immunoreagent is an antibody.
Claim 30
Dependent Claim
12 words
The immunoassay of claim 22 wherein said first immunoreagent is an antigen.
Claim 31
Dependent Claim
16 words
The immunoassay of claim 22 wherein said solvent is a C 1 -C 4 aliphatic alcohol.
Claim 32
Dependent Claim
10 words
The immunoassay of claim 28 wherein said alcohol is ethanol.
Claim 33
Dependent Claim
22 words
The immunoassay of claim 22 wherein solvent rinsing is carried out at a temperature equal to the boiling point of the solvent.
Claim 34
Dependent Claim
23 words
The immunoassay of claim 22 wherein solvent rinsing is carried out for a period of from about one-half hour to about four days.
Claim 35
Dependent Claim
16 words
The immunoassay of claim 31 wherein solvent rinsing is carried out for at least one day.
Claim 36
Dependent Claim
21 words
The immunoassay of claim 22 wherein water rinsing is carried out at a temperature equal to the boiling point of water.
Claim 37
Dependent Claim
16 words
The immunoassay of claim 22 wherein the surface of the carrier is dried after water rinsing.
Claim 38
Dependent Claim
18 words
The immunoassay of claim 34 wherein said drying step is carried at a temperature greater than 200° C.
Claim 39
Dependent Claim
19 words
The immunoassay of claim 35 wherein said drying step is carried out at a temperature of about 300° C.
Claim 40
Dependent Claim
66 words
An element for use in the solid phase thermochemiluminescent immunoassay of claim 22, comprising an immunoreagent immobilized on the surface of a carrier comprised of an inert synthetic resin selected from the group consisting of polyfluorinated synthetic resins, wherein the surface of the carrier has been prepared by thorough washing with a water-miscible organic solvent followed by thorough washing with water before immobilizing the immunoreagent thereon.
Claim 41
Dependent Claim
19 words
The element of claim 37 wherein said polyfluorinated synthetic resin is a polymer of a polyfluorinated ethylenically unsaturated hydrocarbon.
Claim 42
Dependent Claim
12 words
The element of claim 38 wherein said polyfluorinated synthetic resin is polytetrafluoroethylene.
Claim 43
Dependent Claim
11 words
The element of claim 37 wherein said immunoreagent is a protein.
Claim 44
Dependent Claim
11 words
The element of claim 37 wherein said immunoreagent is an antibody.
Claim 45
Dependent Claim
11 words
The element of claim 37 wherein said immunoreagent is an antigen.
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