US4360358A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immunoassay with solid phase having coating containing blood platelet substitute
Assignee: PANBAXY LABORATORIES INTERNATIONAL Corp
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Filing: 1980-03-07
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Granted: 1982-11-23
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Expires: 2000-03-07
Abstract
Abstract A method for preparing an immunologically active coated solid phase, suitably in the form of a coated tube or a polymer latex, for solid phase immuno assays, in particular radio immuno assays, is disclosed. The method comprises coating a solid support which has an organic polymer surface with a coating solution containing the immunologically active agent, e.g., an antibody or antigen, a hydroxy lower alkyl amine, preferably tris(hydroxymethyl) amino methane, and a platelet substitute. The solid support can be a solid organic polymer, such as polystyrene, which can be coated directly with this coating solution. If the immunologically active agent has a molecular weight of below 20000, the organic polymer surface of the solid phase is a matrix of a gel forming hydrophilic polymer. In this case the platelet substitute and the hydroxylower alkyl amine can be omitted from the coating solution.
Inventors
- 👤 Yash Sharma
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 19:35
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/86; G01N33/53; G01N33/543; G01N33/544; Y; Y10; Y10S; Y10S435/00; Y10S435/961; Y10S436/00; Y10S436/807; Y10S436/808
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
183 words
A method for preparing an immunologically active coated solid phase by coating the surface of a solid support material having an organic polymer surface with an immunologically active agent which comprises the steps of: (a) contacting the organic polymer surface of the solid support material with an aqueous coating solution having a pH-value of from about 8.5 to about 10.5 and comprising dissolved therein an amount of from about 0.035 to about 0.05 mol/l of a water soluble protein-compatible primary or secondary hydroxy lower alkyl amine, an amount of between 0.05 and about 20 g/l of a water/soluble immunologically active agent and an amount of from about 0.05 to about 10 g/l of a blood platelet substitute which is a brain extract containing phospholipids and cephalin for a sufficient period of time to allow an immunologically effective amount of the immunologically active agent to be attached to the organic polymer surface of the solid support material to obtain an immunologically active coating therein, (b) removing the coating solution from the coated solid support material (c) allowing the coated solid support material to dry.
Claim 2
Independent Claim
183 words
A method for preparing an immunologically active coated solid phase by coating the surface of a solid support material having an organic polymer surface with an immunologically active agent which comprises the steps of: (a) contacting the organic polymer surface of the solid support material with an aqueous coating solution having a pH-value of from about 8.5 to about 10.5 and comprising dissolved therein an amount of from about 0.035 to about 0.05 mol/l of a water soluble protein-compatible primary or secondary hydroxy lower alkyl amine, an amount of between 0.05 and about 20 g/l of a water/soluble immunologically active agent and an amount of from about 0.05 to about 10 g/l of a blood platelet substitute which is a brain extract containing phospholipids and cephalin for a sufficient period of time to allow an immunologically effective amount of the immunologically active agent to be attached to the organic polymer surface of the solid support material to obtain an immunologically active coating therein, (b) removing the coating solution from the coated solid support material (c) allowing the coated solid support material to dry.
Claim 3
Dependent Claim
32 words
The method as defined in claim 1 wherein the immunologically active agent has a molecular weight of at least 20000 and the solid support material is a water insoluble solid organic polymer.
Claim 4
Dependent Claim
23 words
The method as defined in claim 2 wherein the immunologically active agent has a molecular weight of between about 50000 and about 200000.
Claim 5
Dependent Claim
53 words
The method as defined in claim 2 wherein the immunologically active agent has a molecular weight of between about 20000 and about 200000 and which further comprises the step of pretreating the surface of the solid organic prepolymer with an aqueous solution of a water soluble dialdehyde containing 3 to 8 carbon atoms.
Claim 6
Dependent Claim
48 words
The method as defined in claim 1 wherein the immunologically active agent has a molecular weight of between about 5000 and about 200000 and the organic polymer surface of the support material is a polymer matrix which is a film coating of a protein-compatible hydrophylic gel forming polymer.
Claim 7
Dependent Claim
20 words
The method as defined in claim 1 wherein the platelet substituted further comprises a catalytic amount of a Hageman-factor activator.
Claim 8
Dependent Claim
132 words
A method for determining immunologically active components in an aqueous liquid which comprises the steps of (a) contacting an immunologically active coated phase at least a portion of the surface of which is coated with an immunologically active anti-agent for said immunologically active component by the method as defined in claim 1 with an aqueous solution comprising a sample of said liquid and a known amount of a labeled form of said immunologically active component for a sufficient period of time for an immunological binding reaction to take place whereby portions of the labeled and unlabeled immunologically active component are bound onto the immunologically active solid phase (b) separating said solution from said solid phase and (c) determining the amount of a labeled component in the solid phase or in the solution.
Claim 9
Dependent Claim
100 words
A method for detecting an immunologically active agent in an aqueous liquid which comprises the steps of (a) contacting a suspendable particulate immunologically active coated phase, the surface of which is coated with the immunologically active agent by the method as defined in claim 1 with an aqueous solution comprising a sample of said liquid and a known amount of an immunologically active anti-agent for said immunologically active agent for a sufficient period of time for an immunological binding reaction to take place, (b) determining the amount of agglutination between the particulate immunologically active phase and the immunologically active anti-agent.
Claim 10
Dependent Claim
102 words
A means for carrying out solid phase immuno assays which is in the form of a package which comprises a first container containing an immunologically active coated solid phase, the surface of which is coated with a first immunologically active agent by the method as defined in claim 1; a second container which comprises a second immunologically active agent which is capable of an immunological binding reaction with said first immunologically active agent; and a third container which comprises a labeled form of said second immunologically active agent or an agent which is capable of reacting with said second immunologically active agent.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-11-22 | 1986-05-24 | 1986-11-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-11-22 | 1990-05-24 | 1990-11-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-11-22 | 1994-05-24 | 1994-11-23 | $7700.00 | Window Closed / Lapsed |