US4264571A
Expired / Lapsed
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Radioimmunoassay of thymosin α1
Assignee: George Washington University
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Filing: 1979-01-22
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Granted: 1981-04-28
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Expires: 1999-01-22
Abstract
Abstract An immunoassay for the polypeptidic thymic hormone thymosin α 1 is described. Determination of thymosin α 1 levels in biological fluids, particularly serum, provides a useful diagnostic test for immune deficiency diseases, autoimmune diseases, immunologically mediated diseases, neoplastic diseases and also allows one to monitor therapy with thymosin α 1 by following blood levels of the hormone.
Inventors
- 👤 Allan L. Goldstein
- 👤 John E. McClure
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 22:09
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/26; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; A; A61; A61K; A61K39/00; Y; Y10; Y10S; Y10S436/00; Y10S436/811; Y10S436/813; Y10S436/815; Y10S436/817; Y10S436/823
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
15 words
An immunogen consisting essentially of thymosin α 1 covalently bonded to an immunogenic carrier material.
Claim 2
Dependent Claim
15 words
An immunogen consisting essentially of thymosin α 1 covalently bonded to an immunogenic carrier material.
Claim 3
Dependent Claim
13 words
The immunogen of claim 1 wherein said immunogenic carrier material is a protein.
Claim 4
Dependent Claim
10 words
The immunogen of claim 2 wherein said protein is hemocyanin.
Claim 5
Dependent Claim
26 words
The immunogen of claim 3 wherein said thymosin α 1 is covalently bonded to said immunogenic carrier material through a C 2-7 dialkanal bifunctional linking group.
Claim 6
Dependent Claim
11 words
The immunogen of claim 4 wherein said linking group is glutaraldehyde.
Claim 7
Dependent Claim
35 words
An antibody specific to thymosin α 1 , said thymosin α 1 specific antibody being prepared by innoculating a host animal with the immunogen of claim 1 and collecting the serum from said host animal.
Claim 8
Dependent Claim
5 words
125 I-thymosin α 1 .
Claim 9
Dependent Claim
5 words
125 I-thymosin α 1 .
Claim 10
Independent Claim
126 words
A method for the assay of thymosin α 1 in a sample, which method comprises mixing said sample with a known amount of labelled thymosin α 1 and an antibody which will selectively complex with said thymosin α 1 , separating the resulting antibody-antigen complex from uncomplexed labelled thymosin α 1 , measuring the degree of binding of the said labelled thymosin α 1 in said complex and determining the amount of thymosin α 1 present in said sample by comparing said degree of binding to a standard curve obtained by mixing known amounts of thymosin α 1 with fixed amounts of said labelled thymosin α 1 , and said antibody and determining the degree of binding for each known amount of thymosin α 1 .
Claim 11
Independent Claim
126 words
A method for the assay of thymosin α 1 in a sample, which method comprises mixing said sample with a known amount of labelled thymosin α 1 and an antibody which will selectively complex with said thymosin α 1 , separating the resulting antibody-antigen complex from uncomplexed labelled thymosin α 1 , measuring the degree of binding of the said labelled thymosin α 1 in said complex and determining the amount of thymosin α 1 present in said sample by comparing said degree of binding to a standard curve obtained by mixing known amounts of thymosin α 1 with fixed amounts of said labelled thymosin α 1 , and said antibody and determining the degree of binding for each known amount of thymosin α 1 .
Claim 12
Dependent Claim
17 words
The method of claim 8 wherein a radioimmunoassay is employed and radiolabelled thymosin α 1 is used.
Claim 13
Dependent Claim
17 words
The method of claim 9 wherein said radiolabelled thymosin α 1 is 125 I-thymosin α 1 .
Claim 14
Dependent Claim
44 words
The method of claim 8 wherein the antibody-antigen complex is separated from solution with the assistance of a double antibody technique using a second antibody immunologically reactive against the serum of the hose animal in which the thymosin α 1 specific antibody was elicited.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-04-27 | 1984-10-27 | 1985-04-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-04-27 | 1988-10-27 | 1989-04-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-04-27 | 1992-10-27 | 1993-04-28 | $7700.00 | Window Closed / Lapsed |