US4278651A
Expired / Lapsed
US
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Supported receptor and use thereof in an assay
Assignee: Becton Dickinson and Co
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Filing: 1978-09-27
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Granted: 1981-07-14
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Expires: 1998-09-27
Abstract
Abstract A supported receptor for use in an assay for a ligand in which the solid support includes a water insoluble polymer including at least one functional group which is either carboxyl, isothiocyanate, N-hydroxysuccinimide, imidazolide, bromoacetyl, maleimide, or diazomethylene, with the receptor being covalently linked to the support through the functional group. The preferred support is a particulate support including an impervious non-porous core and an outer porous coating having the water insoluble polymer, including the aforesaid functional groups, adhering thereto.
Inventors
- 👤 Richard H. Hales
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:56
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; A; A61; A61K; A61K39/00; A61K39/395; A61K39/44; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/808
Claims Analysis
Total Claims: 35
Claim 1
Independent Claim
55 words
A supported receptor for use in an assay for a ligand, comprising: a solid support comprising a water insoluble polymer, said polymer including at least one functional group selected from the group consisting of N-hydroxysuccinimide, imidazolide, maleimide, and diazomethylene and a receptor for binding the ligand covalently linked to the support through said functional group.
Claim 2
Dependent Claim
55 words
A supported receptor for use in an assay for a ligand, comprising: a solid support comprising a water insoluble polymer, said polymer including at least one functional group selected from the group consisting of N-hydroxysuccinimide, imidazolide, maleimide, and diazomethylene and a receptor for binding the ligand covalently linked to the support through said functional group.
Claim 3
Dependent Claim
12 words
The supported receptor of claim 1 wherein the functional group is N-hydroxysuccinimide.
Claim 4
Dependent Claim
12 words
The supported receptor of claim 1 wherein the functional group is imidazolide.
Claim 5
Dependent Claim
12 words
The supported receptor of claim 1 wherein the functional group is maleimide.
Claim 6
Dependent Claim
12 words
The supported receptor of claim 1 wherein the functional group is diazomethylene.
Claim 7
Dependent Claim
34 words
The supported receptor of claim 1 wherein the water insoluble polymer is selected from the group consisting of dextran, cellulose, carboxymethyl cellulose, polyacrylic acid, polyallylamine, polyallyl alcohol, polyacrylamide, polyallyl mercaptan, polyallyl cyanide, and polyacrylonitrile.
Claim 8
Dependent Claim
78 words
A supported receptor for use in an assay for a ligand, comprising: a particulate support including an imprevious non-porous core, and an outer porous coating, said particulate support having a water insoluble polymer coating adhering thereto, said water insoluble polymer coating including at least one functional group selected from the group consisting of carboxyl, isothiocyanate, N-hydroxysuccinimide, imidazolide, bromoacetyl, maleimide and diazomethylene, and a receptor for binding the ligand being covalently linked to the support through said functional group.
Claim 9
Dependent Claim
78 words
A supported receptor for use in an assay for a ligand, comprising: a particulate support including an imprevious non-porous core, and an outer porous coating, said particulate support having a water insoluble polymer coating adhering thereto, said water insoluble polymer coating including at least one functional group selected from the group consisting of carboxyl, isothiocyanate, N-hydroxysuccinimide, imidazolide, bromoacetyl, maleimide and diazomethylene, and a receptor for binding the ligand being covalently linked to the support through said functional group.
Claim 10
Dependent Claim
29 words
The supported receptor of claim 7 wherein the water insoluble polymer is selected from the groups consisting of dextran, cellulose and carboxymethyl cellulose and the functional group is carboxyl.
Claim 11
Dependent Claim
33 words
The supported receptor of claim 7 wherein the water insoluble polymer is a polyallylamine which is derivatized to provide functional groups selected from the groups consisting of isothiocyanate, carboxyl, maleimide, diazomethylene and bromoacetyl.
Claim 12
Dependent Claim
23 words
The supported receptor of claim 7 wherein the water insoluble polymer is a polyallyl cyanide which is derivatized to provide carboxyl functional groups.
Claim 13
Dependent Claim
23 words
The supported receptor of claim 7 wherein the water insoluble polymer is a polyallyl mercaptan which is derivatized to provide maleimide functional groups.
Claim 14
Dependent Claim
30 words
The supported receptor of claim 7 wherein the water insoluble polymer is a polyacrylamide which is derivatized to provide functional groups selected from the group consisting of carboxyl and bromoacetyl.
Claim 15
Dependent Claim
21 words
The supported receptor of claim 7 wherein the water insoluble polymer is polyacrylonitrile which is derivatized to provide carboxyl functional groups.
Claim 16
Dependent Claim
21 words
The supported receptor of claim 7 wherein the water insoluble polymer is a polyallyl alcohol derivatized to provide carboxyl functional groups.
Claim 17
Dependent Claim
21 words
The supported receptor of claim 7 wherein the water insoluble polymer is a polyacrylic acid derivatized to provide carboxyl functional groups.
Claim 18
Dependent Claim
29 words
The supported receptor of claim 7 wherein the water insoluble polymer is a polyacrylic acid derivatized to provide functional groups selected from the group consisting of N-hydroxysuccinimide and imidazolide.
Claim 19
Dependent Claim
22 words
The supported receptor of claim 7 wherein the non-porous core is glass spheres and the outer porous coating is formed of microparticles.
Claim 20
Dependent Claim
19 words
The supported receptor of claim 17 wherein the water insoluble polymer is adhered to the support through vinyl groups.
Claim 21
Dependent Claim
19 words
The supported receptor of claim 17 wherein the water insoluble polymer is adhered to the support through silane linkages.
Claim 22
Dependent Claim
34 words
The supported receptor of claim 17 wherein the water insoluble polymer is selected from the group consisting of dextran, cellulose, carboxymethyl cellulose, polyacrylic acid, polyallylamine, polyallyl alcohol, polyacrylamide, polyallyl mercaptan, polyallyl cyanide, and polyacrylonitrile.
Claim 23
Dependent Claim
31 words
In an assay for a ligand employing a tracer and a receptor for binding the ligand, the improvement comprising: employing as the receptor a supported receptor as defined in claim 7.
Claim 24
Dependent Claim
27 words
The assay of claim 21 wherein the assay includes regeneration of the receptor with an eluting solution to effect release of ligand and tracer from the receptor.
Claim 25
Dependent Claim
36 words
In a flow-through chamber for use in an assay for a ligand including a supported receptor for binding the ligand, the improvement comprising: employing as the supported receptor a supported receptor as defined in claim 7.
Claim 26
Dependent Claim
15 words
The supported receptor of claim 1 wherein the water insoluble polymer is on a substrate.
Claim 27
Dependent Claim
37 words
In a flow through chamber for use in an assay for a ligand including a supported receptor for binding the ligand, the improvement comprising: employing as the supported receptor a supported receptor as defined in claim 8.
Claim 28
Dependent Claim
37 words
In a flow through chamber for use in an assay for a ligand including a supported receptor for binding the ligand, the improvement comprising: employing as the supported receptor a supported receptor as defined in claim 9.
Claim 29
Dependent Claim
37 words
In a flow through chamber for use in an assay for a ligand including a supported receptor for binding the ligand, the improvement comprising: employing as the supported receptor a supported receptor as defined in claim 10.
Claim 30
Dependent Claim
37 words
In a flow through chamber for use in an assay for a ligand including a supported receptor for binding the ligand, the improvement comprising: employing as the supported receptor a supported receptor as defined in claim 11.
Claim 31
Dependent Claim
37 words
In a flow through chamber for use in an assay for a ligand including a supported receptor for binding the ligand, the improvement comprising: employing as the supported receptor a supported receptor as defined in claim 12.
Claim 32
Dependent Claim
37 words
In a flow through chamber for use in an assay for a ligand including a supported receptor for binding the ligand, the improvement comprising: employing as the supported receptor a supported receptor as defined in claim 13.
Claim 33
Dependent Claim
37 words
In a flow through chamber for use in an assay for a ligand including a supported receptor for binding the ligand, the improvement comprising: employing as the supported receptor a supported receptor as defined in claim 14.
Claim 34
Dependent Claim
37 words
In a flow through chamber for use in an assay for a ligand including a supported receptor for binding the ligand, the improvement comprising: employing as the supported receptor a supported receptor as defined in claim 15.
Claim 35
Dependent Claim
37 words
In a flow through chamber for use in an assay for a ligand including a supported receptor for binding the ligand, the improvement comprising: employing as the supported receptor a supported receptor as defined in claim 16.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-07-13 | 1985-01-12 | 1985-07-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-07-13 | 1989-01-12 | 1989-07-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-07-13 | 1993-01-12 | 1993-07-14 | $7700.00 | Window Closed / Lapsed |