Immunoassay with article having support film and immunological counterpart of analyte

Claim 2

An immunoassay of total immunoglobulin (Ig) in a biological fluid containing said Ig, which comprises; extracting the Ig from the fluid by binding the Ig to an immobilized immunological counterpart of the Ig wherein the immunological counterpart of the Ig is immobilized by a covalent bonding to a dried film of a light-transparent, synthetic, polymeric resin having attached chemical groups capable of forming covalent bonds with the immunological counterpart; said film being a coating on a water-insoluble, light transparent synthetic, organic polymer article which is inert to chemical reaction with immunoglobulin (Ig); complexing the bound Ig with enzyme-conjugated anti-Ig; mixing the complex with a chromogenic reagent capable of developing color in the presence of the enzyme; and observing the degree of color developed in the mixture.

Claim 3

The immunoassay of claim 1 wherein the immunological counterpart is a compound selected from the group consisting of proteins, lipids, and carbohydrates.

Claim 4

The immunoassay of claim 1 wherein the chemical group is selected from the group consisting of carboxyl, amino, sulfhydryl, amido, mercapto, and hydroxyl groups.

Claim 5

The immunoassay of claim 1 wherein the resin is a light-transparent film of carboxylated styrene-butadiene copolymer.

Claim 6

An immunoassay of an allergen specific immunoglobulin E (IgE) in a biological fluid containing said IgE, which consists essentially of; extracting the IgE from the fluid by binding the IgE to an immobilized allergen immunological counterpart of the IgE, wherein the immunological counterpart of the IgE is immobilized by a covalent bonding to the dried film of a light-transparent, synthetic, polymeric resin having attached chemical groups capable of forming covalent bonds with the immunological counterpart; said film being a coating on a water-insoluble, light-transparent, synthetic organic polymer article which is inert to chemical reaction with immunoglobulin E (IgE); complexing the bound IgE with enzyme-conjugated anti-IgE; mixing the complex with a chromogenic reagent capable of developing color in the presence of the enzyme; and observing the degree of color developed in the mixture.

Claim 7

An immunoassay of an allergen specific immunoglobulin E (IgE) in a biological fluid containing said IgE, which consists essentially of; extracting the IgE from the fluid by binding the IgE to an immobilized allergen immunological counterpart of the IgE, wherein the immunological counterpart of the IgE is immobilized by a covalent bonding to the dried film of a light-transparent, synthetic, polymeric resin having attached chemical groups capable of forming covalent bonds with the immunological counterpart; said film being a coating on a water-insoluble, light-transparent, synthetic organic polymer article which is inert to chemical reaction with immunoglobulin E (IgE); complexing the bound IgE with enzyme-conjugated anti-IgE; mixing the complex with a chromogenic reagent capable of developing color in the presence of the enzyme; and observing the degree of color developed in the mixture.

Claim 8

The immunoassay of claim 5 wherein the resin is a light-transparent copolymer of carboxylated styrene-butadiene.

Claim 9

The immunoassay of claim 6 wherein said fluid is blood serum.

Claim 10

An article for immobilizing reactants of an immunoreaction comprising: (a) a solid-phase, water-insoluble, light-transparent support made of a synthetic, organic polymeric resin; (b) a dried film of light-transparent, carboxylated styrene-butadiene copolymer adhering to the support of (a); and (c) an immuno-active composition covalently immobilized to the film of (b).

Claim 11

An article for immobilizing reactants of an immunoreaction comprising: (a) a solid-phase, water-insoluble, light-transparent support made of a synthetic, organic polymeric resin; (b) a dried film of light-transparent, carboxylated styrene-butadiene copolymer adhering to the support of (a); and (c) an immuno-active composition covalently immobilized to the film of (b).

Claim 12

An immunoassay of an allergen specific immunoglobulin E (IgE) in a biological fluid containing said IgE, which comprises; extracting the IgE from the fluid by binding the IgE to an immobilized allergen immunological counterpart of the IgE, said immobilized allergen being covalently bound to a film of a light-transparent carboxylated copolymer of styrene-butadiene disposed on the sidewall of a light-transparent well in a titer plate fabricated from a synthetic, organic polymeric resin; complexing the bound IgE with enzyme-conjugated anti-IgE; mixing the complex with a chromogenic reagent capable of developing color in the presence of the enzyme; and observing the degree of color developed in the mixture.

Claim 13

An immunoassay of an allergen specific immunoglobulin E (IgE) in a biological fluid containing said IgE, which comprises; extracting the IgE from the fluid by binding the IgE to an immobilized allergen immunological counterpart of the IgE, said immobilized allergen being covalently bound to a film of a light-transparent carboxylated copolymer of styrene-butadiene disposed on the sidewall of a light-transparent well in a titer plate fabricated from a synthetic, organic polymeric resin; complexing the bound IgE with enzyme-conjugated anti-IgE; mixing the complex with a chromogenic reagent capable of developing color in the presence of the enzyme; and observing the degree of color developed in the mixture.