US4716110A
Expired / Lapsed
US
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Composition for assaying hydrogen peroxide
Assignee: Eiken Chemical Co Ltd
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Filing: 1984-10-24
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Granted: 1987-12-29
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Expires: 2004-10-24
Abstract
Abstract The present invention provides a composition for assaying hydrogen peroxide having an enzyme, a buffer and a chromogen, wherein the chromogen is a sulfoalkyl derivative of a 3,3',5,5'-tetraalkylbenzidine or a water-soluble salt thereof represented by the following general formula I: ##STR1## wherein R 1 , R 2 , R 3 and R 4 may be the same or different and each represent a straight-chain alkyl group having 1 to 6 carbon atoms, such as a methyl, ethyl, n-propyl, n-butyl, n-amyl or n-hexyl group. R 5 and R 6 each represent a hydrogen atom or a sulfoalkyl group of the following formula II: --(CH.sub.2).sub.n SO.sub.3 H (II) wherein n represents an integer of 1 to 6, with the proviso that either R 5 and R 6 represents a sulfoalkyl group and the sulfoalkyl group may be substituted with at least 1, preferably 1 to 2 hydroxyl groups. This composition has a high solubility in water and is usable over a wide pH range and for the preparation of both a test solution and a test piece. Various modifications of the composition are possible. Therefore, by using the composition, components of a body fluid can be assayed quantitatively in a precise, easy and rapid manner.
Inventors
- 👤 Hiroshi Wada
- 👤 Yuzo Kosaka
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 10:19
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; C12Q2326/00; C12Q2326/10; C12Q2326/12; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
143 words
A composition for assaying hydrogen peroxide comprising an enzyme, a buffer and a chromogen, wherein said chromogen comprises a sulfoalkyl derivative of a 3,3',5,5'-tetraalkylbenzidine or a water-soluble salt thereof represented by the following general formula I: ##STR3## wherein R 1 , R 2 , R 3 and R 4 are straight-chain alkyl groups having 1 to 6 carbon atoms, selected from the group consisting of a methyl, ethyl, n-propyl, n-butyl, n-amyl and n-hexyl group, and one of R 5 and R 6 represents a sulfoalkyl group of the following formula II: --(CH.sub.2).sub.n SO.sub.3 H (II) wherein n represents an integer of 1 to 6 and wherein the sulfoalkyl group is substituted with at least one hydroxyl group, the other one of R 5 and R 6 representing a member selected from the the group consisting of a hydrogen atom and said sulfoalkyl group.
Claim 2
Dependent Claim
143 words
A composition for assaying hydrogen peroxide comprising an enzyme, a buffer and a chromogen, wherein said chromogen comprises a sulfoalkyl derivative of a 3,3',5,5'-tetraalkylbenzidine or a water-soluble salt thereof represented by the following general formula I: ##STR3## wherein R 1 , R 2 , R 3 and R 4 are straight-chain alkyl groups having 1 to 6 carbon atoms, selected from the group consisting of a methyl, ethyl, n-propyl, n-butyl, n-amyl and n-hexyl group, and one of R 5 and R 6 represents a sulfoalkyl group of the following formula II: --(CH.sub.2).sub.n SO.sub.3 H (II) wherein n represents an integer of 1 to 6 and wherein the sulfoalkyl group is substituted with at least one hydroxyl group, the other one of R 5 and R 6 representing a member selected from the the group consisting of a hydrogen atom and said sulfoalkyl group.
Claim 3
Dependent Claim
20 words
A composition for assaying hydrogen peroxide according to claim 1, wherein said chromogen of the general formula I is N,N'-bis(2-hydroxy-2-sulfoethyl)-3,3',5,5'-tetramethylbenzidine.
Claim 4
Dependent Claim
24 words
A composition for assaying hydrogen peroxide according to claim 1, wherein said enzyme is selected from the group consisting of glucose oxidase and peroxidase.
Claim 5
Dependent Claim
31 words
A composition for assaying hydrogen peroxide according to claim 1, wherein said enzyme, said buffer and said chromogen of the general formula I or water-soluble salt thereof are dissolved in water.
Claim 6
Dependent Claim
31 words
A composition for assaying hydrogen peroxide according to claim 1, wherein said chromogen of the general formula I is a compound selected from the group consisting of N-(2-hydroxy-3-sulfopropyl)-3,3',5,5'-tetramethylbenzidine, N,N'-bis(2-hydroxy-2-sulfoethyl)-3,3',5,5'-tetramethylbenzidine, and N,N'-bis(2-hydroxy-3-sulfopropyl)-3,3',5,5'-tetramethylbenzidine.
Claim 7
Dependent Claim
159 words
A test strip for assaying hydrogen peroxide comprising a composition for assaying hydrogen peroxide supported on an absorptive carrier applied to a support, wherein said composition comprises an enzyme, a buffer and a chromogen comprising a sulfoalkyl derivative of a 3,3',5,5'-tetraalkylbenzidine or a water-soluble salt thereof represented by the following general formula I: ##STR4## wherein R 1 , R 2 , R 3 and R 4 are straight-chain alkyl groups having 1 to 6 carbon atoms, selected from the group consisting of a methyl, ethyl, n-propyl, n-butyl, n-amyl and n-hexyl group, and one of R 5 and R 6 represents a sulfoalkyl group of the following formula II: --(CH.sub.2).sub.n SO.sub.3 H (II) wherein n represents an integer of 1 to 6 and wherein the sulfoalkyl group is substituted with at least one hydroxyl group, the other one of R 5 and R 6 representing a member selected from the group consisting of a hydrogen atom and said sulfoalkyl group.
Claim 8
Dependent Claim
159 words
A test strip for assaying hydrogen peroxide comprising a composition for assaying hydrogen peroxide supported on an absorptive carrier applied to a support, wherein said composition comprises an enzyme, a buffer and a chromogen comprising a sulfoalkyl derivative of a 3,3',5,5'-tetraalkylbenzidine or a water-soluble salt thereof represented by the following general formula I: ##STR4## wherein R 1 , R 2 , R 3 and R 4 are straight-chain alkyl groups having 1 to 6 carbon atoms, selected from the group consisting of a methyl, ethyl, n-propyl, n-butyl, n-amyl and n-hexyl group, and one of R 5 and R 6 represents a sulfoalkyl group of the following formula II: --(CH.sub.2).sub.n SO.sub.3 H (II) wherein n represents an integer of 1 to 6 and wherein the sulfoalkyl group is substituted with at least one hydroxyl group, the other one of R 5 and R 6 representing a member selected from the group consisting of a hydrogen atom and said sulfoalkyl group.
Claim 9
Dependent Claim
21 words
A test strip for assaying hydrogen peroxide according to claim 6, wherein said chromogen of the general formula I is N,N'-bis(2-hydroxy-2-sulfoethyl)-3,3',5,5'-tetramethylbenzidine.
Claim 10
Dependent Claim
18 words
A test strip for assaying hydrogen peroxide according to claim 6, wherein said absorptive carrier is filter paper.
Claim 11
Dependent Claim
18 words
A test strip for assaying hydrogen peroxide according to claim 6, wherein said support is a plastic sheet.
Claim 12
Dependent Claim
167 words
A process for assaying hydrogen peroxide, comprising the steps of: preparing a composition for assaying hydrogen peroxide comprising an enzyme, a buffer and a chromogen, wherein said chromogen comprises a sulfoalkyl derivative of a 3,3',5,5'-tetraalkylbenzidine or a water-soluble salt thereof represented by the following general formula I: ##STR5## wherein R 1 , R 2 , R 3 and R 4 are straight-chain alkyl groups having 1 to 6 carbon atoms, selected from the group consisting of a methyl, ethyl, n-propyl, n-butyl, n-amyl and n-hexyl group, and one of R 5 and R 6 represents a sulfoalkyl group of the following formula II: --(CH.sub.2).sub.n SO.sub.3 H (II) wherein n represents an integer of 1 to 6 and wherein the sulfoalkyl group is substituted with at least one hydroxyl group, the other one of R 5 and R 6 representing a member selected from the group consisting of a hydrogen atom and said sulfoalkyl group; and contacting a solution with said composition, thereby assaying the solution for hydrogen peroxide.
Claim 13
Dependent Claim
167 words
A process for assaying hydrogen peroxide, comprising the steps of: preparing a composition for assaying hydrogen peroxide comprising an enzyme, a buffer and a chromogen, wherein said chromogen comprises a sulfoalkyl derivative of a 3,3',5,5'-tetraalkylbenzidine or a water-soluble salt thereof represented by the following general formula I: ##STR5## wherein R 1 , R 2 , R 3 and R 4 are straight-chain alkyl groups having 1 to 6 carbon atoms, selected from the group consisting of a methyl, ethyl, n-propyl, n-butyl, n-amyl and n-hexyl group, and one of R 5 and R 6 represents a sulfoalkyl group of the following formula II: --(CH.sub.2).sub.n SO.sub.3 H (II) wherein n represents an integer of 1 to 6 and wherein the sulfoalkyl group is substituted with at least one hydroxyl group, the other one of R 5 and R 6 representing a member selected from the group consisting of a hydrogen atom and said sulfoalkyl group; and contacting a solution with said composition, thereby assaying the solution for hydrogen peroxide.
Claim 14
Dependent Claim
187 words
A process for assaying hydrogen peroxide comprising the steps of: preparing a test strip for assaying hydrogen peroxide comprising a composition for assaying hydrogen peroxide supported on an absorptive carrier applied to a support, wherein said composition comprises an enzyme, a buffer and a chromogen, said chromogen comprising a sulfoalkyl derivative of a 3,3',5,5'-tetraalkylbenzidine or a water-soluble salt thereof represented by the following general formula I: ##STR6## wherein R 1 , R 2 , R 3 and R 4 are straight-chain alkyl groups having 1 to 6 carbon atoms, selected from the group consisting of a methyl, ethyl, n-propyl, n-butyl, n-amyl and n-hexyl group, and one of R 5 and R 6 represents a sulfoalkyl group of the following formula II: --(CH.sub.2).sub.n SO.sub.3 H (II) wherein n represents an integer of 1 to 6 and wherein the sulfoalkyl group is substituted with at least one hydroxyl group, the other one of R 5 and R 6 representing a member selected from the group consisting of a hydrogen atom and said sulfoalkyl group; and contacting a solution with said test strip, thereby assaying the solution for hydrogen peroxide.
Claim 15
Dependent Claim
187 words
A process for assaying hydrogen peroxide comprising the steps of: preparing a test strip for assaying hydrogen peroxide comprising a composition for assaying hydrogen peroxide supported on an absorptive carrier applied to a support, wherein said composition comprises an enzyme, a buffer and a chromogen, said chromogen comprising a sulfoalkyl derivative of a 3,3',5,5'-tetraalkylbenzidine or a water-soluble salt thereof represented by the following general formula I: ##STR6## wherein R 1 , R 2 , R 3 and R 4 are straight-chain alkyl groups having 1 to 6 carbon atoms, selected from the group consisting of a methyl, ethyl, n-propyl, n-butyl, n-amyl and n-hexyl group, and one of R 5 and R 6 represents a sulfoalkyl group of the following formula II: --(CH.sub.2).sub.n SO.sub.3 H (II) wherein n represents an integer of 1 to 6 and wherein the sulfoalkyl group is substituted with at least one hydroxyl group, the other one of R 5 and R 6 representing a member selected from the group consisting of a hydrogen atom and said sulfoalkyl group; and contacting a solution with said test strip, thereby assaying the solution for hydrogen peroxide.
Claim 16
Dependent Claim
129 words
A composition for assaying hydrogen peroxide comprising an enzyme, a buffer and a chromogen, wherein said chromogen comprises a sulfoalkyl derivative of a 3,3',5,5'-tetraalkylbenzidine or a water-soluble salt thereof represented by the following general formula I: ##STR7## wherein R 1 , R 2 , R 3 and R 4 are straight-chain alkyl groups having 1 to 6 carbon atoms, selected from the group consisting of a methyl, ethyl, n-propyl, n-butyl, n-amyl and n-hexyl group, and one of R 5 and R 6 represents a sulfoalkyl group of the following formula II: --(CH.sub.2).sub.n SO.sub.3 H (II) wherein n represents an integer of 1 to 6, the other one of R 5 and R 6 representing a member selected from the group consisting of a hydrogen atom and said sulfoalkyl group.
Claim 17
Dependent Claim
129 words
A composition for assaying hydrogen peroxide comprising an enzyme, a buffer and a chromogen, wherein said chromogen comprises a sulfoalkyl derivative of a 3,3',5,5'-tetraalkylbenzidine or a water-soluble salt thereof represented by the following general formula I: ##STR7## wherein R 1 , R 2 , R 3 and R 4 are straight-chain alkyl groups having 1 to 6 carbon atoms, selected from the group consisting of a methyl, ethyl, n-propyl, n-butyl, n-amyl and n-hexyl group, and one of R 5 and R 6 represents a sulfoalkyl group of the following formula II: --(CH.sub.2).sub.n SO.sub.3 H (II) wherein n represents an integer of 1 to 6, the other one of R 5 and R 6 representing a member selected from the group consisting of a hydrogen atom and said sulfoalkyl group.
Claim 18
Dependent Claim
39 words
A composition for assaying hydrogen peroxide according to claim 12, wherein said chromogen of the general formula I is a compound selected from the group consisting of the following compounds: N-(2-sulfoethyl)-3,3',5,5'-tetramethylbenzidine, N-(3-sulfopropyl)-3,3',5,5'-tetramethylbenzidine, N-(4-sulfobutyl)-3,3',5,5'-tetramethylbenzidine, N-(3-sulfopropyl)-3,3',5,5'-tetraethylbenzidine, N,N'-bis(2-sulfoethyl)-3,3',5,5'-tetramethylbenzidine, N,N'-bis(3-sulfopropyl)-3,3',5,5'-tetramethylbenzidine, N,N'-bis(4-sulfobutyl)-3,3',5,5'-tetramethylbenzidine, and N,N'-bis(3-sulfopropyl)-3,3',5,5'-tetramethylbenzidine.
Claim 19
Dependent Claim
20 words
A composition for assaying hydrogen peroxide according to claim 12, wherein said chromogen of the general formula I is N-(3-sulfopropyl)-3,3',5,5'-tetramethylbenzidine.
Claim 20
Dependent Claim
148 words
A test strip for assaying hydrogen peroxide comprising a composition for assaying hydrogen peroxide supported on an absorptive carrier applied to a support, wherein said composition comprises an enzyme, a buffer and a chromogen, said chromogen comprising a sulfoalkyl derivative of a 3,3',5,5'-tetraalkylbenzidine or a water-soluble salt thereof represented by the following general formula I: ##STR8## wherein R 1 , R 2 , R 3 and R 4 are straight-chain alkyl groups having 1 to 6 carbon atoms, selected from the group consisting of a methyl, ethyl, n-propyl, n-butyl, n-amyl and n-hexyl group, and one of R 5 and R 6 represents a sulfoalkyl group of the following formula II: --(CH.sub.2).sub.n SO.sub.3 H (II) wherein n represents an integer of 1 to 6, the other one of R 5 and R 6 representing a member selected from the group consisting of a hydrogen atom and said sulfoalkyl group.
Claim 21
Dependent Claim
148 words
A test strip for assaying hydrogen peroxide comprising a composition for assaying hydrogen peroxide supported on an absorptive carrier applied to a support, wherein said composition comprises an enzyme, a buffer and a chromogen, said chromogen comprising a sulfoalkyl derivative of a 3,3',5,5'-tetraalkylbenzidine or a water-soluble salt thereof represented by the following general formula I: ##STR8## wherein R 1 , R 2 , R 3 and R 4 are straight-chain alkyl groups having 1 to 6 carbon atoms, selected from the group consisting of a methyl, ethyl, n-propyl, n-butyl, n-amyl and n-hexyl group, and one of R 5 and R 6 represents a sulfoalkyl group of the following formula II: --(CH.sub.2).sub.n SO.sub.3 H (II) wherein n represents an integer of 1 to 6, the other one of R 5 and R 6 representing a member selected from the group consisting of a hydrogen atom and said sulfoalkyl group.
Claim 22
Dependent Claim
21 words
A test strip for assaying hydrogen peroxide according to claim 15, wherein said chromogen of the general formula I is N-(3-sulfopropyl)-3,3',5,5'-tetramethylbenzidine.
Claim 23
Dependent Claim
139 words
A process for assaying hydrogen peroxide, comprising the steps of: preparing a composition for assaying hydrogen peroxide comprising an enzyme, a buffer and a chromogen, wherein said chromogen comprises a sulfoalkyl derivative of 3,3',5,5'-tetraalkylbenzidine or a water-soluble salt thereof represented by the following general formula I: ##STR9## wherein R 1 , R 2 , R 3 and R 4 are straight-chain alkyl groups having 1 to 6 carbon atoms, selected from the group consisting of a methyl, ethyl, n-propyl, n-butyl, n-amyl and n-hexyl group, and one of R 5 and R 6 represents a sulfoalkyl group of the following formula II: --(CH.sub.2).sub.n SO.sub.3 H (II) wherein n represents an integer of 1 to 6, the other one of R 5 and R 6 representing a member selected from the group consisting of a hydrogen atom and said sulfoalkyl group.
Claim 24
Dependent Claim
139 words
A process for assaying hydrogen peroxide, comprising the steps of: preparing a composition for assaying hydrogen peroxide comprising an enzyme, a buffer and a chromogen, wherein said chromogen comprises a sulfoalkyl derivative of 3,3',5,5'-tetraalkylbenzidine or a water-soluble salt thereof represented by the following general formula I: ##STR9## wherein R 1 , R 2 , R 3 and R 4 are straight-chain alkyl groups having 1 to 6 carbon atoms, selected from the group consisting of a methyl, ethyl, n-propyl, n-butyl, n-amyl and n-hexyl group, and one of R 5 and R 6 represents a sulfoalkyl group of the following formula II: --(CH.sub.2).sub.n SO.sub.3 H (II) wherein n represents an integer of 1 to 6, the other one of R 5 and R 6 representing a member selected from the group consisting of a hydrogen atom and said sulfoalkyl group.
Claim 25
Dependent Claim
159 words
A process for assaying hydrogen peroxide, comprising the steps of: preparing a test strip for assaying hydrogen peroxide comprising a composition for assaying hydrogen peroxide supported on an absorptive carrier applied to a support, wherein said composition comprises an enzyme, a buffer and a chromogen, said chromogen comprising a sulfoalkyl derivative of a 3,3',5,5'-tetraalkylbenzidine or a water-soluble salt thereof represented by the following general formula I: ##STR10## wherein R 1 , R 2 , R 3 and R 4 are straight-chain alkyl groups having 1 to 6 carbon atoms, selected from the group consisting of a methyl, ethyl, n-propyl, n-butyl, n-amyl and n-hexyl group, and one of R 5 and R 6 represents a sulfoalkyl group of the following formula II: --(CH.sub.2).sub.n SO.sub.3 H (II) wherein n represents an integer of 1 to 6, the other one of R 5 and R 6 representing a member selected from the group consisting of a hydrogen atom and said sulfoalkyl group.
Claim 26
Dependent Claim
159 words
A process for assaying hydrogen peroxide, comprising the steps of: preparing a test strip for assaying hydrogen peroxide comprising a composition for assaying hydrogen peroxide supported on an absorptive carrier applied to a support, wherein said composition comprises an enzyme, a buffer and a chromogen, said chromogen comprising a sulfoalkyl derivative of a 3,3',5,5'-tetraalkylbenzidine or a water-soluble salt thereof represented by the following general formula I: ##STR10## wherein R 1 , R 2 , R 3 and R 4 are straight-chain alkyl groups having 1 to 6 carbon atoms, selected from the group consisting of a methyl, ethyl, n-propyl, n-butyl, n-amyl and n-hexyl group, and one of R 5 and R 6 represents a sulfoalkyl group of the following formula II: --(CH.sub.2).sub.n SO.sub.3 H (II) wherein n represents an integer of 1 to 6, the other one of R 5 and R 6 representing a member selected from the group consisting of a hydrogen atom and said sulfoalkyl group.
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