US4361648A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Color fixed chromogenic analytical element
Assignee: Bayer Corp
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Filing: 1981-08-13
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Granted: 1982-11-30
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Expires: 2001-08-13
Abstract
Abstract Analytical element method of making the analytical element and process for determining an analyte in a body fluid sample are disclosed. More particularly, the method of preparing the element comprises the steps of: (a) impregnating a carrier with a first solution, having a pH not greater than about 2.5, which comprises a polymeric mordant and a tetraalkylbenzidine dihydrochloride, in a molar concentration greater than that of the polymeric mordant in an aqueous solvent and drying the carrier; (b) impregnating the carrier of (a) with a solution, having a pH of at least about 7.0, which comprises an analyte-responsive component in an aqueous solvent and drying the carrier. Optionally, there can be added the step of impregnating the carrier of (b) with a solution of a semi-permeable polymer in an organic solvent and drying the carrier.
Inventors
- 👤 Chen Shuenn-tzong
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 06:36
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/521; C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; C12Q2326/00; C12Q2326/10; C12Q2326/12; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
125 words
A method for preparing an analytical element for determining an analyte in a liquid sample which method comprises the steps of: (a) impregnating a carrier with a first solution having a pH not greater than about 2.5, which comprises a polymeric mordant present in a concentration from about 0.5 to about 0.75 percent of the first solution on a weight to weight basis, and a tetraalkylbenzidine dihydrocholoride, with the tetraalkylbenzidine dihydrochloride in a molar concentration greater than that of the polymeric mordant, in an aqueous solvent and drying the carrier; (b) impregnating the carrier of (a) with a second solution, having a pH of at least about 7.0, which comprises an enzyme and a peroxidatively active substance in an aqueous solvent and drying the carrier.
Claim 2
Independent Claim
125 words
A method for preparing an analytical element for determining an analyte in a liquid sample which method comprises the steps of: (a) impregnating a carrier with a first solution having a pH not greater than about 2.5, which comprises a polymeric mordant present in a concentration from about 0.5 to about 0.75 percent of the first solution on a weight to weight basis, and a tetraalkylbenzidine dihydrocholoride, with the tetraalkylbenzidine dihydrochloride in a molar concentration greater than that of the polymeric mordant, in an aqueous solvent and drying the carrier; (b) impregnating the carrier of (a) with a second solution, having a pH of at least about 7.0, which comprises an enzyme and a peroxidatively active substance in an aqueous solvent and drying the carrier.
Claim 3
Dependent Claim
16 words
The method of claim 1 wherein the polymeric mordant is a methylvinyl ether maleic anhydride copolymer.
Claim 4
Dependent Claim
12 words
The method of claim 1 wherein the polymeric mordant is polyacrylic acid.
Claim 5
Dependent Claim
13 words
The method of claim 1 wherein the polymeric mordant is an acrylate-acrylamide copolymer.
Claim 6
Dependent Claim
15 words
The method of claim 1 wherein the polymeric mordant is a vinyl acetate-maleic anhydride copolymer.
Claim 7
Dependent Claim
12 words
The method of claim 1 wherein the tetraalkylbenzidine dihydrochloride is 3,3',5,5'-tetramethylbenzidine dihydrochloride.
Claim 8
Dependent Claim
32 words
The method of claim 1 wherein the tetraalkylbenzidine dihydrochloride is present in a concentration of from about 1.0 to about 2.0 percent of the first solution on a weight to weight basis.
Claim 9
Dependent Claim
22 words
The method of claim 1 wherein the enzyme and the peroxidatively active substance comprises an analyte-specific oxidase and a peroxidatively active substance.
Claim 10
Dependent Claim
18 words
The method of claim 1 wherein the enzyme and the peroxidatively active substance comprises glucose oxidase and peroxidase.
Claim 11
Dependent Claim
18 words
The method of claim 1 wherein the enzyme and the peroxidatively active substance comprises cholesterol oxidase and peroxidase.
Claim 12
Dependent Claim
20 words
The method of claim 1 wherein the enzyme and the peroxidatively active substance comprises cholesterol oxidase, cholesterol esterase and peroxidase.
Claim 13
Dependent Claim
17 words
The method of claim 1 wherein the enzyme and the peroxidatively active substance comprises uricase and peroxidase.
Claim 14
Dependent Claim
20 words
The method of claim 1 wherein the enzyme and the peroxidatively active substance comprises an amino acid oxidase and peroxidase.
Claim 15
Dependent Claim
18 words
The method of claim 1 wherein the enzyme and the peroxidatively active substance comprises xanthine oxidase and peroxidase.
Claim 16
Dependent Claim
32 words
The method of claim 1 which comprises the additional step of (c) impregnating the carrier of (b) with a solution of a semi-permeable polymer in an organic solvent and drying the carrier.
Claim 17
Dependent Claim
11 words
The method of claim 15 wherein the organic solvent includes toluene.
Claim 18
Dependent Claim
13 words
The method of claim 15 wherein the organic solvent includes toluene and ethanol.
Claim 19
Dependent Claim
43 words
The method of claim 15 wherein the organic solvent consists essentially of toluene, from about 80 to about 95 percent of the solvent, and ethanol, from about 5 to about 20 percent of the solvent, the toluene and ethanol being together 100 percent.
Claim 20
Independent Claim
175 words
A method for preparing an analytical element for determining glucose in a liquid sample which method comprises the steps of: (a) impregnating a carrier with a first solution, having a pH not greater than about 2.5, which comprises an interpolymer of methyl vinyl ether and maleic anhydride present in a concentration from about 0.5 to about 0.75 percent of the first solution on a weight to weight basis and 3,3',5,5'-tetramethylbenzidine dihydrochloride, in a molar concentration greater than that of the interpolymer, in an aqueous solvent and drying the carrier; (b) impregnating the carrier of (a) with a solution, having a pH of at least about 7.0, which comprises glucose oxidase and peroxidase in an aqueous solvent and drying the carrier; and (c) impregnating the carrier of (b) with a solution of ethyl cellulose in a solvent which consists essentially of toluene, from about 80 to about 95 percent of the solvent, and ethanol, from about 5 to about 20 percent of the solvent, the toluene and ethanol being together 100 percent, and drying the carrier.
Claim 21
Independent Claim
175 words
A method for preparing an analytical element for determining glucose in a liquid sample which method comprises the steps of: (a) impregnating a carrier with a first solution, having a pH not greater than about 2.5, which comprises an interpolymer of methyl vinyl ether and maleic anhydride present in a concentration from about 0.5 to about 0.75 percent of the first solution on a weight to weight basis and 3,3',5,5'-tetramethylbenzidine dihydrochloride, in a molar concentration greater than that of the interpolymer, in an aqueous solvent and drying the carrier; (b) impregnating the carrier of (a) with a solution, having a pH of at least about 7.0, which comprises glucose oxidase and peroxidase in an aqueous solvent and drying the carrier; and (c) impregnating the carrier of (b) with a solution of ethyl cellulose in a solvent which consists essentially of toluene, from about 80 to about 95 percent of the solvent, and ethanol, from about 5 to about 20 percent of the solvent, the toluene and ethanol being together 100 percent, and drying the carrier.
Claim 22
Dependent Claim
26 words
An analytical element for determining an analyte in a liquid sample, which element is prepared by the method of any of claims 1, 15 or 19.
Claim 23
Independent Claim
36 words
A method for determining an analyte in a liquid sample which comprises contacting the sample with an analytical element prepared by the method of any of claims 1, 15 or 19 and observing any detectable response.
Claim 24
Independent Claim
176 words
A method for preparing an analytical element for determining cholesterol in a liquid sample which method comprises the steps of: (a) impregnating a carrier with a first solution having a pH not greater than about 2.5, which comprises an interpolymer of methyl vinyl ether and maleic anhydride present in a concentration from about 0.5 to about 0.75% of the first solution on a weight to weight basis and 3,3',5,5'-tetramethylbenzidine dihydrocholoride, with the 3,3',5,5'-tetramethylbenzidine in a molar concentration greater than that of the interpolymer, in an aqueous solvent and drying the carrier; (b) impregnating the carrier of (a) with a solution, having a pH of at least about 7.0, which comprises cholesterol oxidase, cholesterol esterase and peroxidase in an aqueous solvent and drying the carrier; and (c) impregnating the carrier of (b) with a solution of ethyl cellulose and a solvent which consists essentially of toluene, from about 80 to about 95% of the solvent, and ethanol, from about 5 to about 20% of the solvent, the toluene and ethanol being together 100%, and drying the carrier.
Claim 25
Independent Claim
176 words
A method for preparing an analytical element for determining cholesterol in a liquid sample which method comprises the steps of: (a) impregnating a carrier with a first solution having a pH not greater than about 2.5, which comprises an interpolymer of methyl vinyl ether and maleic anhydride present in a concentration from about 0.5 to about 0.75% of the first solution on a weight to weight basis and 3,3',5,5'-tetramethylbenzidine dihydrocholoride, with the 3,3',5,5'-tetramethylbenzidine in a molar concentration greater than that of the interpolymer, in an aqueous solvent and drying the carrier; (b) impregnating the carrier of (a) with a solution, having a pH of at least about 7.0, which comprises cholesterol oxidase, cholesterol esterase and peroxidase in an aqueous solvent and drying the carrier; and (c) impregnating the carrier of (b) with a solution of ethyl cellulose and a solvent which consists essentially of toluene, from about 80 to about 95% of the solvent, and ethanol, from about 5 to about 20% of the solvent, the toluene and ethanol being together 100%, and drying the carrier.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-11-29 | 1986-05-31 | 1986-11-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-11-29 | 1990-05-31 | 1990-11-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-11-29 | 1994-05-31 | 1994-11-30 | $7700.00 | Window Closed / Lapsed |