Patent US4613501A successfully refreshed from Google Patents.
US4613501A
Expired / Lapsed
US
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Inactivation of viruses in labile blood derivatives
Assignee: New York Blood Center
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Filing: 1984-12-21
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Granted: 1986-09-23
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Expires: 2004-12-21
Abstract
Abstract There is disclosed a process for obtaining a protein-containing composition which is substantially free of lipid-containing viruses without incurring substantial protein denaturation comprising contacting said protein-containing composition with an effective amount of oleic acid, its ester or alkali or alkaline earth metal salt for a sufficient period of time to inactivate virus contained therein.
Inventors
- 👤 Bernard Horowitz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 23:30
CPC Classifications
C; C12; C12N; C12N7/00; A; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A61K35/16; A61L; A61L2/00; A61L2/02; A61L2/04; A61L2/16; A61L2/18; C07; C07K; C07K14/00; C07K14/435; C07K14/745; C07K14/755; A61K38/00; A61L2103/00; A61L2103/05; C12N2760/00; C12N2760/00011; C12N2760/20011; C12N2760/20211; C12N2760/20261; C12N2760/20263; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83; Y10S530/831
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
59 words
A process for rendering a blood product which comprises a labile protein substantially free of lipid-containing viruses without incurring substantial protein denaturation comprising contacting said product with an effective amount of C 1 -C 4 -alkyl oleic acid, its ester or alkali or alkaline earth metal salt thereof for a sufficient period of time to inactivate virus contained therein.
Claim 2
Dependent Claim
59 words
A process for rendering a blood product which comprises a labile protein substantially free of lipid-containing viruses without incurring substantial protein denaturation comprising contacting said product with an effective amount of C 1 -C 4 -alkyl oleic acid, its ester or alkali or alkaline earth metal salt thereof for a sufficient period of time to inactivate virus contained therein.
Claim 3
Dependent Claim
13 words
A process according to claim 1 wherein an oleic acid salt is employed.
Claim 4
Dependent Claim
12 words
A process according to claim 2 wherein said salt in sodium oleate.
Claim 5
Dependent Claim
11 words
A process according to claim 1 wherein oleic acid is employed.
Claim 6
Dependent Claim
18 words
A process according to claim 1 wherein said contacting is conducted in the presence of a wetting agent.
Claim 7
Dependent Claim
14 words
A process according to claim 5 wherein said wetting agent is a non-ionic detergent.
Claim 8
Dependent Claim
29 words
A process according to claim 5 wherein said wetting agent is added to said protein-containing composition prior to contacting said protein-containing composition with said oleic acid, ester or salt.
Claim 9
Dependent Claim
24 words
A process according to claim 5 wherein said wetting agent is added simultaneously with said oleic acid, ester or salt to said protein-containing composition.
Claim 10
Dependent Claim
23 words
A process according to claim 5 wherein said wetting agent is added after said oleic acid, ester or salt contacts said protein-containing composition.
Claim 11
Dependent Claim
16 words
A process according to claim 6 wherein said detergent is a partial ester of sorbitol anhydrides.
Claim 12
Dependent Claim
27 words
A process according to claim 1 further comprising conducting said contacting in the presence of an inactivating agent selected from the group consisting of ethers and alcohols.
Claim 13
Dependent Claim
27 words
A process according to claim 5 further comprising conducting said contacting in the presence of an inactivation agent selected from the group consisting of ethers and alcohols.
Claim 14
Dependent Claim
55 words
A process according to claim 1 wherein said product is a blood product selected from the group consisting of whole blood, blood plasma, a plasma concentrate, a precipitate from any fractionation of such plasma, a supernatant from any fractionation of said plasma, a serum, a cryoprecipitate, a cell lysate, and proteins induced in blood cells.
Claim 15
Dependent Claim
57 words
A process according to claim 1 wherein said product contains one or more proteins selected from the group consisting of fibrinogen, factor II, factor VII, factor VIII, factor IX, factor X, factor I, immunoglobins, prealbumin, retinol-binding protein, albumin, alpha-globulins, beta-globulins, gamma-globulins, factor III and the complement components, fibronectin, antithrombin III, hemoglobin, interferon, T-cell growth factor, plasminogen activator.
Claim 16
Dependent Claim
26 words
A process according to claim 1 wherein said protein-containing composition is the product of a non-blood normal or cancerous cell or the product of gene splicing.
Claim 17
Dependent Claim
18 words
A process according to claim 1 wherein said period of time is between about 1 and 5 hours.
Claim 18
Dependent Claim
23 words
A process according to claim 1 wherein said contacting is conducted at a temperature of between about 0° C. and about 60° C.
Claim 19
Dependent Claim
29 words
A process according to claim 1 wherein said oleic acid, ester or salt is employed in a concentration of between about 0.02 weight percent and about 0.1 weight percent.
Claim 20
Dependent Claim
33 words
A process according to claim 18 wherein said oleic acid, ester or salt is employed in a concentration of between 0.02 weight percent and 0.04 weight percent for 1-4 hours at room temperature.
Claim 21
Dependent Claim
12 words
A process according to claim 18 wherein said product comprises factor VIII.
Claim 22
Dependent Claim
12 words
A process according to claim 18 wherein said product comprises factor IX.
Claim 23
Dependent Claim
37 words
A process according to claim 1, wherein said product has an extent of inactivation of virus of greater than 4 logs of virus and has a yield of protein activity to total protein of at least 70%.
Claim 24
Dependent Claim
19 words
A process according to claim 22, wherein the yield of protein activity to total protein is at least 95%.
Claim 25
Dependent Claim
19 words
A process according to claim 22, wherein the yield of protein activity to total protein is 98% to 100%.
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