US4315919A
Expired / Lapsed
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Depyrogenation process
Assignee: Individual
•
Filing: 1980-10-06
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Granted: 1982-02-16
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Expires: 2000-10-06
Abstract
Abstract Biological and pharmaceutical products which are contaminated with pyrogens are depyrogenated by prolonged contact with a solution or suspension of from about 0.25% to about 10% by weight of a non-denaturing amphiphile.
Inventors
- 👤 Edward Shanbrom
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:02
CPC Classifications
A; A61; A61L; A61L2/00; A61L2/16; A61L2/18; A61K; A61K35/00; A61K35/12; A61K35/14; A61K39/00; A61L2103/00; A61L2103/05
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
88 words
The method of depyrogenating a proteinaceous biological or pharmaceutical product comprising (1) treating said proteinaceous biological or pharmaceutical product by prolonged contact with a solution or suspension of from about 0.25% to about 10% by weight of a non-denaturing amphiphile, (2) adding to the resulting treatment mixture a protein precipitant for said proteinaceous biological or pharmaceutical product to thereby cause precipitation of said proteinaceous product and (3) separating from the resulting precipitate the supernatant which contains said amphiphile together with endotoxin dissociated or disaggregated by said prolonged contact.
Claim 2
Independent Claim
88 words
The method of depyrogenating a proteinaceous biological or pharmaceutical product comprising (1) treating said proteinaceous biological or pharmaceutical product by prolonged contact with a solution or suspension of from about 0.25% to about 10% by weight of a non-denaturing amphiphile, (2) adding to the resulting treatment mixture a protein precipitant for said proteinaceous biological or pharmaceutical product to thereby cause precipitation of said proteinaceous product and (3) separating from the resulting precipitate the supernatant which contains said amphiphile together with endotoxin dissociated or disaggregated by said prolonged contact.
Claim 3
Dependent Claim
25 words
The method of claim 1 in which the amphiphile is a bile acid salt selected from the group consisting of sodium cholate and sodium deoxycholate.
Claim 4
Dependent Claim
13 words
The method of claim 1 in which the amphiphile is a nonionic surfactant.
Claim 5
Dependent Claim
31 words
The method of claim 3 in which the nonionic surfactant is selected from the group consisting of the polyoxyethylated derivatives of partial esters of C 12-22 fatty acids and hexitol anhydrides.
Claim 6
Dependent Claim
13 words
The method of claim 4 in which the nonionic surfactant is Polysorbate 80.
Claim 7
Dependent Claim
45 words
The method of claim 3 in which the nonionic surfactant is selected from the group consisting of substances having the general formula RC 6 H 4 (OC 2 H 4 ) n OH wherein R is octyl or nonyl and n is at least 3.
Claim 8
Dependent Claim
15 words
The method of claim 6 in which the nonionic surfactant is octyl phenoxy polyethoxy ethanol.
Claim 9
Dependent Claim
15 words
The method of claim 7 in which the concentration of the surfactant is about 2-3%.
Claim 10
Dependent Claim
15 words
The method of claim 1 in which the treated product is derived from fermentation processes.
Claim 11
Dependent Claim
14 words
The method of claim 9 in which the amphiphile is octyl phenoxy polyethoxy ethanol.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-02-15 | 1985-08-17 | 1986-02-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-02-15 | 1989-08-17 | 1990-02-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-02-15 | 1993-08-17 | 1994-02-16 | $7700.00 | Window Closed / Lapsed |