US4540573A
Expired / Lapsed
US
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Undenatured virus-free biologically active protein derivatives
Assignee: New York Blood Center
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Filing: 1983-07-14
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Granted: 1985-09-10
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Expires: 2003-07-14
Abstract
Abstract A mammalian blood protein-containing composition such as whole blood, plasma, serum, plasma concentrate, cryoprecipitate, cryosupernatant, plasma fractionation precipitate or plasma fractionation supernatant substantially free of hepatitis and other lipid coated viruses with the yield of protein activity to total protein being at least 80% is disclosed. The protein-containing composition is contacted with di- or trialkylphosphate, preferably a mixture of trialkylphosphate and detergent, usually followed by removal of the di- or trialkylphosphate.
Inventors
- 👤 Alexander R. Neurath
- 👤 Bernard Horowitz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:46
CPC Classifications
A; A61; A61L; A61L2/00; A61L2/02; A61L2/04; A61K; A61K31/00; A61K31/13; A61K31/14; A61K35/00; A61K35/12; A61K35/14; A61K38/00; A61L2/16; A61L2/18; A61L2103/00; A61L2103/05; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83; Y10S530/831
Claims Analysis
Total Claims: 35
Claim 1
Independent Claim
59 words
A process for rendering a blood product which comprises a labile blood protein substantially free of lipid-containing viruses without incurring substantial protein denaturation which comprises contacting said blood product with an effective amount of a di- or trialkylphosphate for a period of time sufficient to render said blood product substantially free of lipid-containing viruses without incurring substantial protein denaturation.
Claim 2
Dependent Claim
59 words
A process for rendering a blood product which comprises a labile blood protein substantially free of lipid-containing viruses without incurring substantial protein denaturation which comprises contacting said blood product with an effective amount of a di- or trialkylphosphate for a period of time sufficient to render said blood product substantially free of lipid-containing viruses without incurring substantial protein denaturation.
Claim 3
Dependent Claim
20 words
A process according to claim 1 wherein di- or trialkylphosphate has alkyl groups which contain 1 to 10 carbon atoms.
Claim 4
Dependent Claim
19 words
A process according to claim 2 wherein said trialkylphosphate has alkyl groups which contain 2 to 10 carbon atoms.
Claim 5
Dependent Claim
12 words
A process according to claim 2 wherein said trialkylphosphate is tri-n-butyl phosphate.
Claim 6
Dependent Claim
18 words
A process according to claim 1 wherein said contacting is conducted in the presence of a wetting agent.
Claim 7
Dependent Claim
14 words
A process according to claim 5 wherein said wetting agent is a non-ionic detergent.
Claim 8
Dependent Claim
27 words
A process according to claim 5 wherein said wetting agent is added to said blood product prior to contacting said blood product with said di- or trialkylphosphate.
Claim 9
Dependent Claim
22 words
A process according to claim 5 wherein said wetting agent is added simultaneously with said di- or trialkylphosphate to said blood product.
Claim 10
Dependent Claim
21 words
A process according to claim 5 wherein said wetting agent is added after said di- or trialkylphosphate contacts said blood product.
Claim 11
Dependent Claim
16 words
A process according to claim 6 wherein said detergent is a partial ester of sorbitol anhydrides.
Claim 12
Dependent Claim
29 words
A process according to claim 1 further comprising conducting said contacting in the presence of lipid coated virus inactivating agent selected from the group consisting of ethers and alcohols.
Claim 13
Dependent Claim
27 words
A process according to claim 5 further comprising conducting said contacting in the presence of an inactivating agent selected from the group consisting of ethers and alcohols.
Claim 14
Dependent Claim
59 words
A process according to claim 1 wherein said blood protein is selected from the group consisting of whole blood, blood plasma, red blood cells, leucocytes, platelet concentrates, a plasma concentrate, a precipitate from any fractionation of such plasma, a supernatant from any fractionation of said plasma, a serum, a cryoprecipitate, a cell lysate and proteins induced in blood cells.
Claim 15
Dependent Claim
88 words
A process according to claim 1 wherein said labile blood protein is selected from the group consisting of fibrinogen, cold insoluble globulin, properdin, IgG, IgM, IgA, betaliproprotein, plasmin-inhibitor, factor V, thrombin, antithrombin, isoagglutinins, cerutoplasmin, alpha 1 -lipoprotein, peptidase, transferrin, thyroxine binding globulin, serum esterase, alkaline phosphates, alpha 1 -acid glycoprotein, factor II, factor VII, factor VIII, factor IX, factor X, factor XIII, factor I, immunoglobulins, prealbumin, retinol-binding protein, albumin, alpha-globulins, beta-globulins, gamma-globulins, factor III, the complement components, fibronectin, antithrombin III, hemoglobin, interferon, T-cell growth factor and plasminogen activator.
Claim 16
Dependent Claim
21 words
A process according to claim 1 wherein following said contacting with said di- or trialkylphosphate, said di- or trialkylphosphate is removed.
Claim 17
Dependent Claim
20 words
A process according to claim 1 wherein said period of time is between about 1 minute and about 30 hours.
Claim 18
Dependent Claim
23 words
A process according to claim 1 wherein said contacting is conducted at a temperature of between about 0° C. and about 70° C.
Claim 19
Dependent Claim
22 words
A process according to claim 1 wherein said di- or trialkylphosphate is present in an amount between about 0.001% and about 1%.
Claim 20
Dependent Claim
13 words
A process according to claim 13 wherein said blood product comprises factor VIII.
Claim 21
Dependent Claim
13 words
A process according to claim 13 wherein said blood product comprises factor IX.
Claim 22
Dependent Claim
23 words
A process according to claim 1 wherein said blood product is additionally heated for at least 5 hours at 50° to 70° C.
Claim 23
Dependent Claim
24 words
A process according to claim 22 wherein the composition which is heated comprises a protein stabilizer which stabilizes a protein against denaturation by heat.
Claim 24
Dependent Claim
14 words
A process according to claim 1 wherein said labile blood protein is factor VIII.
Claim 25
Dependent Claim
14 words
A process according to claim 1 wherein said labile blood protein is factor VII.
Claim 26
Dependent Claim
14 words
A process according to claim 1 wherein said labile blood protein is factor IX.
Claim 27
Dependent Claim
14 words
A process according to claim 1 wherein said labile blood protein is factor X.
Claim 28
Dependent Claim
13 words
A process according to claim 1 wherein said labile blood protein is fibrinogen.
Claim 29
Dependent Claim
13 words
A process according to claim 1 wherein said labile blood protein is fibronectin.
Claim 30
Dependent Claim
17 words
A process according to claim 1 wherein said labile blood protein comprises an antibody against infectious hepatitis.
Claim 31
Dependent Claim
14 words
A process according to claim 1 wherein said labile blood protein is factor XIII.
Claim 32
Dependent Claim
15 words
A process according to claim 1 wherein said labile blood protein is T-cell growth factor.
Claim 33
Dependent Claim
17 words
A process according to claim 1 wherein said labile blood protein is a protein of a leucocyte.
Claim 34
Dependent Claim
19 words
A process according to claim 1 wherein said labile blood protein is a protein of a red blood cell.
Claim 35
Dependent Claim
19 words
A process according to claim 1 wherein said labile blood protein is a protein of a blood platelet concentrate.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-09-09 | 1989-03-11 | 1989-09-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-09-09 | 1993-03-11 | 1993-09-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-09-09 | 1997-03-11 | 1997-09-10 | $7700.00 | Window Closed / Lapsed |