US4495295A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immunoassays using support-containing separate antigens and antibodies derived from an immune complex
Assignee: New York Blood Center
•
Filing: 1982-03-29
•
Granted: 1985-01-22
•
Expires: 2002-03-29
Abstract
Abstract A process for the detection of an antigen or antibody in a specimen which process comprises: (a) contacting said specimen with a substrate having bound thereon a mixture of antigens and antibodies to said antigen or antibody in said specimen, said antibodies and said antigens bound to said substrate being separately bound to said substrate and not in the form of an immune complex, incubating the so-contacted substrate and washing the substrate; (b) contacting the washed material of step `a` with a radioactive material labeled or enzyme labeled antibody or antigen, incubating the so-contacted material and washing the same; and (c) effecting radioimmunoassay if said antibody or antigen is radioactive or enzyme labeled immunoassay is said antibody or antigen is enzyme labeled.
Inventors
- 👤 A. Robert Neurath
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 13:02
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54306; G01N33/54393; G01N33/544; Y; Y10; Y10S; Y10S435/00; Y10S435/966; Y10S435/973; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/82; Y10S436/823
Claims Analysis
Total Claims: 30
Claim 1
Independent Claim
112 words
A process for the detection of an antigen in a specimen which process comprises: (a) contacting said specimen with a substrate having bound thereon a mixture of antigens and antibodies to said antigen in said specimen, said antibodies and said antigens bound to said substrate being separately bound to said substrate and not in the form of an immune complex, incubating the so-contacted substrate and washing the substrate; (b) contacting the washed material of step `a` with a radioactive material labeled or enzyme labeled antibody, incubating the so-contacted material and washing the same; and (c) effecting radioimmunoassay if said antibody is radioactive or enzyme labeled immunoassay is said antibody is enzyme labeled.
Claim 2
Dependent Claim
112 words
A process for the detection of an antigen in a specimen which process comprises: (a) contacting said specimen with a substrate having bound thereon a mixture of antigens and antibodies to said antigen in said specimen, said antibodies and said antigens bound to said substrate being separately bound to said substrate and not in the form of an immune complex, incubating the so-contacted substrate and washing the substrate; (b) contacting the washed material of step `a` with a radioactive material labeled or enzyme labeled antibody, incubating the so-contacted material and washing the same; and (c) effecting radioimmunoassay if said antibody is radioactive or enzyme labeled immunoassay is said antibody is enzyme labeled.
Claim 3
Dependent Claim
78 words
A process according to claim 1, wherein the washed material of step `a` is contacted with a hapten conjugated antibody against said antigen of said specimen, the so-contacted material is incubated and washed and thereafter the resultant washed material is contacted with a radioactive material labeled or enzyme labeled anit-hapten antibody, incubated and washed and radioimmunoassay is effected if said anti-hapten antibody is radio labeled or enzyme labeled immunoassay is effected if said anti-hapten antibody is enzyme labeled.
Claim 4
Dependent Claim
42 words
A process according to claim 1, wherein said substrate having bound thereon a mixture of antigens and antibodies is a protein binding solid support in which the sites free of antigen or antibody are substantially filled with a protein binding site occupier.
Claim 5
Dependent Claim
24 words
A process according to claim 3, wherein said protein site occupier is bovine serum albumin, gelatin, a normal human serum or an animal serum.
Claim 6
Dependent Claim
18 words
A process according to claim 3, wherein said protein binding solid support is derivatized paper or plastic material.
Claim 7
Dependent Claim
14 words
A process according to claim 3, wherein said solid support is a paper derivative.
Claim 8
Dependent Claim
13 words
A process according to claim 6, wherein said paper is a nitrocellulose paper.
Claim 9
Dependent Claim
17 words
A process according to claim 6, wherein said paper is a diazobenzyloxymethyl paper or a diazophenylthioether paper.
Claim 10
Dependent Claim
14 words
A process according to claim 3, wherein said solid support is a plastic material.
Claim 11
Dependent Claim
12 words
A process according to claim 3, wherein said solid support comprises polystyrene.
Claim 12
Dependent Claim
14 words
A process according to claim 3, wherein said solid support comprises a polyvinyl material.
Claim 13
Dependent Claim
13 words
A process according to claim 3, wherein said solid support comprises a polyolefin.
Claim 14
Dependent Claim
133 words
A process for detecting the presence of an antigen in a specimen which comprises: (a) contacting the specimen suspected of containing an antigen with a substrate having bound thereon a mixture of antigens and antibodies to said antigen in said specimen, said antibodies and said antigen being bound to said substrate separately not being in the form of an immune complex, in the presence of a radioactive material labeled or enzyme labeled antigen, incubating the so-contacted substrate and washing the substrate; (b) effecting radioimmunoassay if said antigen is radioactive or enzyme labeled immunoassay if said antigen is enzyme labeled; and (c) comparing the counts if radioimmunoassay is effected or enzymatic activity if enzyme immunoassay is effected with the counts or enzymatic activity of a control carried out without the presence of said specimen.
Claim 15
Dependent Claim
133 words
A process for detecting the presence of an antigen in a specimen which comprises: (a) contacting the specimen suspected of containing an antigen with a substrate having bound thereon a mixture of antigens and antibodies to said antigen in said specimen, said antibodies and said antigen being bound to said substrate separately not being in the form of an immune complex, in the presence of a radioactive material labeled or enzyme labeled antigen, incubating the so-contacted substrate and washing the substrate; (b) effecting radioimmunoassay if said antigen is radioactive or enzyme labeled immunoassay if said antigen is enzyme labeled; and (c) comparing the counts if radioimmunoassay is effected or enzymatic activity if enzyme immunoassay is effected with the counts or enzymatic activity of a control carried out without the presence of said specimen.
Claim 16
Dependent Claim
119 words
A process for the detection of the presence of an antibody in a test specimen which process comprises: (a) contacting said specimen suspected of containing antibody with a substrate having bound thereon a mixture of antibody and antigen to said antibody in said specimen, said antigens and said antibodies bound to said substrate being separately bound to said substrate and not in the form of an immune complex, incubating the so-contacted substrate and washing the substrate; (b) contacting the washed material of step `a` with a radioactive material labeled or enzyme labeled antigen, incubating the same and washing the same; and (c) effecting radioimmunoasssay if said antigen is radioactive or enzyme labeled immunoassay if said antigen is enzyme labeled.
Claim 17
Dependent Claim
119 words
A process for the detection of the presence of an antibody in a test specimen which process comprises: (a) contacting said specimen suspected of containing antibody with a substrate having bound thereon a mixture of antibody and antigen to said antibody in said specimen, said antigens and said antibodies bound to said substrate being separately bound to said substrate and not in the form of an immune complex, incubating the so-contacted substrate and washing the substrate; (b) contacting the washed material of step `a` with a radioactive material labeled or enzyme labeled antigen, incubating the same and washing the same; and (c) effecting radioimmunoasssay if said antigen is radioactive or enzyme labeled immunoassay if said antigen is enzyme labeled.
Claim 18
Dependent Claim
75 words
A process according to claim 14, wherein following step `a`, the washed material of step `a` is contacted with a hapten conjugated antigen against said antibody, the so-contacted material is incubated and washed and the resultant washed material is contacted with a radioactive material labeled or enzyme-containing anti-hapten antibody incubated and washed after which radioimmunoassay is effected if said antibody is radio active or enzyme labeled immunoassay is effected if said antibody is enzyme labeled.
Claim 19
Dependent Claim
34 words
A process according to claim 14, wherein said substrate comprises a protein binding solid support whose protein binding sites not occupied by antigen or antibody are substantially filled with a protein binding site occupier.
Claim 20
Dependent Claim
24 words
A process according to claim 16, wherein said protein site occupier is bovine serum albumin, gelatin, a normal human serum or an animal serum.
Claim 21
Dependent Claim
18 words
A process according to claim 16, wherein said protein binding solid support is derivatized paper or plastic material.
Claim 22
Dependent Claim
15 words
A process according to claim 18, wherein said solid support is a paper (cellulose) derivative.
Claim 23
Dependent Claim
12 words
A process according to claim 18, wherein said support is nitrocellulose paper.
Claim 24
Dependent Claim
18 words
A process according to claim 18, wherein said solid support is a diazobenzyloxymethyl paper or a diazophenylthioether paper.
Claim 25
Dependent Claim
14 words
A process according to claim 18, wherein said solid support comprises a plastic material.
Claim 26
Dependent Claim
14 words
A process according to claim 18, wherein said solid support comprises a polyvinyl material.
Claim 27
Dependent Claim
12 words
A process according to claim 18, wherein said support comprises a polystyrene.
Claim 28
Dependent Claim
12 words
A process according to claim 18, wherein said support comprises a polyolefin.
Claim 29
Dependent Claim
111 words
A process for the detection of the presence of an antibody in a specimen which process comprises: (a) contacting said specimen suspected of containing antibody with a substrate having bound thereon a mixture of antibodies and antigens to said antibody in said specimen, said antigen and said antibodies bound to said substrate being seperately bound to said substrate and not in the form of an immune complex, in the presence of a radioactive material labeled or enzyme labeled antibody, incubating the so-contacted substrate and washing the substrate; (b) comparing the counts or enzymatic activity with the counts or enzymatic activity of a control carried out without the use of said specimen.
Claim 30
Dependent Claim
111 words
A process for the detection of the presence of an antibody in a specimen which process comprises: (a) contacting said specimen suspected of containing antibody with a substrate having bound thereon a mixture of antibodies and antigens to said antibody in said specimen, said antigen and said antibodies bound to said substrate being seperately bound to said substrate and not in the form of an immune complex, in the presence of a radioactive material labeled or enzyme labeled antibody, incubating the so-contacted substrate and washing the substrate; (b) comparing the counts or enzymatic activity with the counts or enzymatic activity of a control carried out without the use of said specimen.
References Cited (Backward Citations)
US3872225A
Cited
US4138213A
Cited
US4172117A
Cited
US4210622A
Cited
US4241175A
Cited
EP0019504A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-01-22 | 1988-07-23 | 1989-01-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-01-22 | 1992-07-23 | 1993-01-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-01-22 | 1996-07-23 | 1997-01-22 | $7700.00 | Window Closed / Lapsed |