US4172117A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for the simultaneous measurement of antigens and their antibodies by solid-phase radioimmunoassay
Assignee: Biotest Serum Institut GmbH
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Filing: 1977-09-30
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Granted: 1979-10-23
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Expires: 1997-09-30
Abstract
Abstract Antigens and their antibodies are measured in a combined radioimmunoassay by incubating a sample of an unknown fluid with a known quantity of an antibody, contacting the mixture obtained with the walls of a vessel having a coating of known antigen content, again incubating, then aspirating the mixture and washing the walls of the vessel, adding a pure radioactive tracer antigen, aspirating and washing again, and finally measuring the radioactivty of the coated parts conventionally, e.g., in a gamma counter known in the art.
Inventors
- 👤 Andreas Schober
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 09:10
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54306; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/819; Y10S436/82
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
109 words
Method of determining the presence of antigen or antibody in an unknown fluid by a solid-phase radioimmunoassay which method comprises incubating a sample of an unknown fluid with a known quantity of an antibody, contacting the mixture obtained with a carrier surface having a coating of known antigen content, again incubating, then aspirating the mixture and washing said carrier surface, adding a pure radioactive tracer antigen, aspirating and washing again, measuring the radioactivity of the coated parts and comparing the radioactive count with the count of a negative control whereby if the count is higher the fluid contains antibody and if the count is lower, the fluid contains antigen.
Claim 2
Dependent Claim
109 words
Method of determining the presence of antigen or antibody in an unknown fluid by a solid-phase radioimmunoassay which method comprises incubating a sample of an unknown fluid with a known quantity of an antibody, contacting the mixture obtained with a carrier surface having a coating of known antigen content, again incubating, then aspirating the mixture and washing said carrier surface, adding a pure radioactive tracer antigen, aspirating and washing again, measuring the radioactivity of the coated parts and comparing the radioactive count with the count of a negative control whereby if the count is higher the fluid contains antibody and if the count is lower, the fluid contains antigen.
Claim 3
Dependent Claim
36 words
Method as claimed in claim 1 wherein the quantity of antibody employed in the incubation of the unknown sample is 10 to 15% of the maximum antibody quantity bindable by the coating of the carrier surface.
Claim 4
Dependent Claim
30 words
Method as claimed in claim 1 wherein the first incubation is carried out for a period from 1 to 16 hours at a temperature of from 20° to 50° C.
Claim 5
Dependent Claim
23 words
Method as claimed in claim 3 wherein the incubation is carried out for about one hour at a temperature of about 37° C.
Claim 6
Dependent Claim
26 words
Method as claimed in claim 3 wherein the incubation is carried out for a period of about 12 hours at a temperature of about 20° C.
Claim 7
Dependent Claim
15 words
Method as claimed in claim 1 wherein micro-titration plates are used as the carrier surface.
Claim 8
Dependent Claim
17 words
Method as claimed in claim 6 wherein said micro-titration plates are fragmented prior to measuring their radioactivity.
Claim 9
Dependent Claim
13 words
Method as claimed in claim 1 wherein said unknown fluid is whole blood.
Claim 10
Dependent Claim
12 words
Method as claimed in claim 1 wherein said unknown fluid is plasma.
Claim 11
Dependent Claim
14 words
Method as claimed in claim 1 wherein said unknown fluid is urine or saliva.
References Cited (Backward Citations)
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US3733398A
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US3826619A
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Cited
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-10-22 | 1983-04-23 | 1983-10-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-10-22 | 1987-04-23 | 1987-10-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-10-22 | 1991-04-23 | 1991-10-23 | $7700.00 | Window Closed / Lapsed |