US4241175A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Assay for hepatitis B core antibody
Assignee: Merck and Co Inc
•
Filing: 1978-12-18
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Granted: 1980-12-23
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Expires: 1998-12-18
Abstract
Abstract HBcAb is detected in biological fluid by contacting HBcAg derived from Dane particles and adhered to a surface with the biological fluid and an antibody-enzyme conjugate. The HBcAg is adhered to the surface in the presence of a nonionic surfactant.
Inventors
- 👤 William J. Miller
- 👤 William J. McAleer
- 👤 Lynn T. Callahan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 15:22
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/576; G01N33/5761; G01N33/5762; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/964
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
114 words
A method for determining the presence of hepatitis B core antibody (HB c Ab) in biological fluid comprising (a) directly adhering hepatitis B core antigen (HB c Ag) derived from Dane particles to a solid surface wherein HB c Ag is adhered to the surface by adsorption in the presence of an amount of a nonionic surfactant having from about 15 to about 35 oxyethylene units effective to cause adherence, (b) contacting the surface with the biological fluid, (c) contacting the surface with a hepatitis B core antibody (HB c Ab)-enzyme conjugate, (d) contacting the surface with an enzyme substrate solution, and (e) measuring the optical density after development of the enzyme substrate solution.
Claim 2
Independent Claim
114 words
A method for determining the presence of hepatitis B core antibody (HB c Ab) in biological fluid comprising (a) directly adhering hepatitis B core antigen (HB c Ag) derived from Dane particles to a solid surface wherein HB c Ag is adhered to the surface by adsorption in the presence of an amount of a nonionic surfactant having from about 15 to about 35 oxyethylene units effective to cause adherence, (b) contacting the surface with the biological fluid, (c) contacting the surface with a hepatitis B core antibody (HB c Ab)-enzyme conjugate, (d) contacting the surface with an enzyme substrate solution, and (e) measuring the optical density after development of the enzyme substrate solution.
Claim 3
Dependent Claim
15 words
A method according to claim 1 wherein the biological fluid is blood, plasma or serum.
Claim 4
Dependent Claim
12 words
A method according to claim 2 wherein the biological fluid is plasma.
Claim 5
Dependent Claim
15 words
A method according to claim 1 wherein the nonionic surfactant is polyoxyethylene (20) sorbitan monooleate.
Claim 6
Dependent Claim
54 words
A diagnostic reagent comprising a solid surface having directly adhered thereto hepatitis B core antigen (HB c Ag) derived from Dane particles wherein HB c Ag is adhered by adsorption in the presence of a nonionic surfactant having from about 15 to about 35 oxyethylene units present in an amount effective to cause adherence.
Claim 7
Dependent Claim
54 words
A diagnostic reagent comprising a solid surface having directly adhered thereto hepatitis B core antigen (HB c Ag) derived from Dane particles wherein HB c Ag is adhered by adsorption in the presence of a nonionic surfactant having from about 15 to about 35 oxyethylene units present in an amount effective to cause adherence.
Claim 8
Dependent Claim
32 words
A diagnostic reagent according to claim 5 wherein the nonionic surfactant is an oxyethylated alkyl phenol, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene acid, polyoxyethylene alcohol, polyoxyethylene oil, or polyoxyethylene oxypropylene fatty acid.
Claim 9
Dependent Claim
26 words
A diagnostic reagent according to claim 5 wherein at least part of the HB c Ag is complexed with hepatitis B core antibody (HB c Ab).
Claim 10
Dependent Claim
24 words
A diagnostic reagent according to claim 5 wherein at least part of the HB c Ag is complexed with an HB c Ab-enzyme conjugate.
Claim 11
Dependent Claim
39 words
A diagnostic reagent according to claim 5 wherein at least part of the HB c Ag is complexed with HB c Ab and at least part of the HB c Ag is complexed with an HB c Ab-enzyme conjugate.
Claim 12
Dependent Claim
13 words
A diagnostic reagent according to claim 7 wherein the enzyme is alkaline phosphatase.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-12-23 | 1984-06-23 | 1984-12-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-12-23 | 1988-06-23 | 1988-12-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-12-23 | 1992-06-23 | 1992-12-23 | $7700.00 | Window Closed / Lapsed |