US4410634A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of passively adsorbing immuno-reactive haptens to solid phases
Assignee: Whittaker Bioproducts Inc
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Filing: 1981-11-12
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Granted: 1983-10-18
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Expires: 2001-11-12
Abstract
Abstract The method comprises covalently binding an immuno-reactive to a selected macromolecular carrier and then contacting the resulting hapten-carrier conjugate at a selected concentration in a liquid phase with a selected solid phase until a desired amount of the hapten-carrier conjugate is adsorbed to the surface of the solid phase. Unbound hapten-carrier conjugate is then separated from the solid phase, and the solid phase containing the bound hapten-carrier conjugate is recovered for use in quantitative immunoassays and the like. The solid phase can be, for example, surfaces of a test tube or microtiter well or the like. The method is simple and inexpensive and permits hapten assays of improved sensitivity.
Inventors
- 👤 Harold R. Cooper
- 👤 Andrew O'Beirne
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 20:50
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; G01N33/74; G01N33/78; Y; Y10; Y10S; Y10S435/00; Y10S435/961; Y10S435/966; Y10S436/00; Y10S436/804; Y10S436/815; Y10S436/823
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
147 words
A method of readily adsorbing immuno-reactive hapten to a solid phase for an immuno-assay, which hapten otherwise would not readily adsorb to said solid phase and retain its immuno-reactivity, which method retains said immuno-reactivity and comprises: a. covalently binding an immuno-reactive hapten selected from the group consisting of digoxin, thyroxin, gentamicin, and triiodothyronine to a macromolecular carrier selected from the group consisting of bovine serum albumin, and human serum albumin through the use of a coupled agent selected from the group consisting of glutaraldehyde, and periodate with sodium borohydride; b. contacting the resulting hapten-carrier conjugate with a solid phase selected from the group consisting of polystyrene, and polyvinyl acetate until a desired concentration of said hapten-carrier conjugate is adsorbed to the surface of said solid phase; and, c. thereafter removing unbound hapten-carrier conjugate from said solid phase and recovering said solid phase containing said bound hapten-carrier conjugate.
Claim 2
Independent Claim
147 words
A method of readily adsorbing immuno-reactive hapten to a solid phase for an immuno-assay, which hapten otherwise would not readily adsorb to said solid phase and retain its immuno-reactivity, which method retains said immuno-reactivity and comprises: a. covalently binding an immuno-reactive hapten selected from the group consisting of digoxin, thyroxin, gentamicin, and triiodothyronine to a macromolecular carrier selected from the group consisting of bovine serum albumin, and human serum albumin through the use of a coupled agent selected from the group consisting of glutaraldehyde, and periodate with sodium borohydride; b. contacting the resulting hapten-carrier conjugate with a solid phase selected from the group consisting of polystyrene, and polyvinyl acetate until a desired concentration of said hapten-carrier conjugate is adsorbed to the surface of said solid phase; and, c. thereafter removing unbound hapten-carrier conjugate from said solid phase and recovering said solid phase containing said bound hapten-carrier conjugate.
Claim 3
Dependent Claim
23 words
The method of claim 1 wherein said hapten-carrier conjugate is in a liquid phase when said contacting with said solid phase is initiated.
Claim 4
Dependent Claim
31 words
The method of claim 2 wherein said hapten-carrier conjugate is diluted to a desired concentration with a liquid diluent and then contacted with said solid phase while still in said diluent.
Claim 5
Dependent Claim
34 words
The method of claim 1 wherein said hapten comprises digoxin, wherein said macromolecular carrier comprises human serum albumin, wherein said coupling reagent comprises periodate and sodium borohydride, and wherein said solid phase comprises polystyrene.
Claim 6
Dependent Claim
31 words
The method of claim 1 wherein said hapten comprises thyroxin, wherein said macromolecular carrier comprises bovine serum albumin, wherein said coupling reagent comprises glutaraldehyde, and wherein said solid phase comprises polystyrene.
Claim 7
Dependent Claim
31 words
The method of claim 1 wherein said hapten comprises gentamicin, wherein said macromolecular carrier comprises bovine serum albumin, wherein said coupling reagent comprises glutaraldehyde, and wherein said solid phase comprises polystyrene.
Claim 8
Dependent Claim
33 words
The method of claim 1 wherein said hapten comprises triiodothyronine, wherein said macromolecular carrier comprises bovine serum albumin, wherein said coupling reagent comprises gluta aldehyde, and wherein said solid phase comprises polyvinyl acetate.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-10-17 | 1987-04-18 | 1987-10-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-10-17 | 1991-04-18 | 1991-10-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-10-17 | 1995-04-18 | 1995-10-18 | $7700.00 | Window Closed / Lapsed |