US4001583A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Covalently bound biological substances to plastic materials and use in radioassay
Assignee: Individual
•
Filing: 1974-10-04
•
Granted: 1977-01-04
•
Expires: 1994-10-04
Abstract
Abstract Covalently bound biological substances to plastic materials whose inside surfaces have been coated with glutaraldehyde, with or without prior treatment with an aliphatic amine or diamine, useful in radioimmunoassay procedures.
Inventors
- 👤 M. James Barrett
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:31
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/78; C; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; G01N33/53; G01N33/543; G01N33/54353; G01N33/82; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/81
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
46 words
A method of covalently binding a biological substance to a plastic material which comprises reacting the inside surface of said plastic material with an aliphatic primary amine or diamine, then treating said surface with glutaraldehyde followed by coupling said biological substance to the glutaraldehyde treated surface.
Claim 2
Independent Claim
46 words
A method of covalently binding a biological substance to a plastic material which comprises reacting the inside surface of said plastic material with an aliphatic primary amine or diamine, then treating said surface with glutaraldehyde followed by coupling said biological substance to the glutaraldehyde treated surface.
Claim 3
Dependent Claim
13 words
The method of claim 1 in which the biological substance is a protein.
Claim 4
Dependent Claim
21 words
The method of claim 1 in which the biological substance is a specific binding protein of natural occurrence or an antibody.
Claim 5
Dependent Claim
13 words
The method of claim 1 in which the biological substance is an enzyme.
Claim 6
Dependent Claim
14 words
The method of claim 1 in which the plastic material is polyethylene or polypropylene.
Claim 7
Dependent Claim
37 words
The method of claim 1 in which the aliphatic amine is CH 3 (CH 2 ) 17 NH 2 and the aliphatic diamine is CH 3 (CH 2 ) 17 NH(CH 2 ) 3 NH 2 .
Claim 8
Dependent Claim
19 words
The method of claim 1 in which the plastic material is in the form of a length of tubing.
Claim 9
Dependent Claim
18 words
The method of claim 1 in which the plastic material is in the form of a test tube.
Claim 10
Independent Claim
128 words
A method of radioassaying a specimen for a biological substance which comprises using a plastic test tube, the inside surface of which has been coated with glutaraldehyde and an immunological counterpart specific for the biological substance to be assayed has been covalently coupled thereto, incubating in said test tube a solution containing a buffer, a radioactively labeled derivative of the biological substance to be assayed and the unlabeled biological substance to be assayed from a specimen or from a standard solution of said substance, decanting the incubation mixture, counting with a radioactive detection device the amount of radioactive labeled derivative of said substance remaining on the inside of said test tube or remaining in the incubation solution and comparing the count with that obtained from a standard curve.
Claim 11
Independent Claim
128 words
A method of radioassaying a specimen for a biological substance which comprises using a plastic test tube, the inside surface of which has been coated with glutaraldehyde and an immunological counterpart specific for the biological substance to be assayed has been covalently coupled thereto, incubating in said test tube a solution containing a buffer, a radioactively labeled derivative of the biological substance to be assayed and the unlabeled biological substance to be assayed from a specimen or from a standard solution of said substance, decanting the incubation mixture, counting with a radioactive detection device the amount of radioactive labeled derivative of said substance remaining on the inside of said test tube or remaining in the incubation solution and comparing the count with that obtained from a standard curve.
Claim 12
Dependent Claim
12 words
The method of claim 9 in which the biological substance is thyroxine.
Claim 13
Dependent Claim
13 words
The method of claim 9 in which the biological substance is folic acid.
Claim 14
Dependent Claim
15 words
The method of claim 9 in which the biological substance is Vitamin B 12 .
Claim 15
Dependent Claim
50 words
A radioassay kit for radioassaying a specimen for a biological substance comprising: a. a plastic test tube, the inside surface of which is coated with glutaraldehyde; b. an immunological counterpart of said biological substance covalently coupled to said glutaraldehyde treated surface; and c. labeled biological substance capable of emitting radiation.
Claim 16
Dependent Claim
50 words
A radioassay kit for radioassaying a specimen for a biological substance comprising: a. a plastic test tube, the inside surface of which is coated with glutaraldehyde; b. an immunological counterpart of said biological substance covalently coupled to said glutaraldehyde treated surface; and c. labeled biological substance capable of emitting radiation.
Claim 17
Dependent Claim
13 words
The radioassay kit of claim 13 in which the biological substance is thyroxine.
Claim 18
Dependent Claim
14 words
The radioassay kit of claim 13 in which the biological substance is folic acid.
Claim 19
Dependent Claim
16 words
The radioassay kit of claim 13 in which the biological substance is Vitamin B 12 .
References Cited (Backward Citations)
GB1257263A
Cited
US3720760A
Cited
US3720760B1
Cited
US3646346A
Cited
US3708572A
Cited
US3790663A
Cited
US3745211A
Cited
US3826619A
Cited
US3845202A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-01-04 | 1980-07-05 | 1981-01-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-01-04 | 1984-07-05 | 1985-01-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-01-04 | 1988-07-05 | 1989-01-04 | $7700.00 | Window Closed / Lapsed |