US4100268A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Preparation of gentamicin sensitized particles for agglutination tests
Assignee: Technam Inc
•
Filing: 1976-03-08
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Granted: 1978-07-11
•
Expires: 1996-03-08
Abstract
Abstract Detection of gentamicin is made possible by preparing an analytical body comprising a macromolecule which carries gentamicin, attached to a particle.
Inventors
- 👤 George H. Scherr
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 20:25
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; G01N33/9446; Y; Y10; Y10S; Y10S436/00; Y10S436/815; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/815; Y10S530/816; Y10S530/827; Y10S530/829
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
53 words
A process for the preparation of an agglutinable gentamicin-macromolecule-carrying particle which process comprises covalently coupling gentamicin to a protein with a carbodiimide derivative and adsorbing or covalently coupling the resultant gentamicin-protein moiety to washed red blood cells, resulting in a particle consisting essentially of the protein, the red blood cell and coupled gentamicin.
Claim 2
Dependent Claim
53 words
A process for the preparation of an agglutinable gentamicin-macromolecule-carrying particle which process comprises covalently coupling gentamicin to a protein with a carbodiimide derivative and adsorbing or covalently coupling the resultant gentamicin-protein moiety to washed red blood cells, resulting in a particle consisting essentially of the protein, the red blood cell and coupled gentamicin.
Claim 3
Dependent Claim
17 words
A process as claimed in claim 1 in which the red blood cells are preserved with formaldehyde.
Claim 4
Dependent Claim
18 words
A process as claimed in claim 1 in which the red blood cells are sheep red blood cells.
Claim 5
Dependent Claim
25 words
A process as claimed in claim 1 in which the gentamicin-macromolecule moiety is adsorbed to red blood cells which have been treated with tannic acid.
Claim 6
Dependent Claim
21 words
A process as claimed in claim 1 in which the protein to which Gentamicin is covalently coupled is bovine serum albumin.
Claim 7
Dependent Claim
21 words
A process as claimed in claim 1 in which the protein to which gentamicin is covalently coupled is goat gamma globulin.
Claim 8
Dependent Claim
22 words
A process as claimed in claim 1 in which the gentamicin-protein moiety is covalently coupled to the red blood cells with bisdiazobenzidine.
Claim 9
Dependent Claim
34 words
A process for the preparation of an agglutinable gentamicin-macromolecule-carrying particle which process comprises covalently coupling gentamicin to a protein with a carbodiimide derivative and adsorbing or covalently coupling said gentamicin-protein moiety to microbial cells.
Claim 10
Dependent Claim
34 words
A process for the preparation of an agglutinable gentamicin-macromolecule-carrying particle which process comprises covalently coupling gentamicin to a protein with a carbodiimide derivative and adsorbing or covalently coupling said gentamicin-protein moiety to microbial cells.
Claim 11
Dependent Claim
15 words
A process as claimed in claim 8 in which the microbial cells are yeast cells.
Claim 12
Dependent Claim
21 words
A process as claimed in claim 8 in which the gentamicin-macromolecule moiety is covalently coupled to the yeast cells with bisdiazobenzidine.
Claim 13
Dependent Claim
21 words
A process as claimed in claim 8 in which the macromolecule to which gentamicin is covalently coupled is bovine serum albumin.
Claim 14
Dependent Claim
16 words
A process as claimed in claim 8 in which the microbial cells are preserved with formaldehyde.
Claim 15
Dependent Claim
35 words
A process for the preparation of an agglutinable gentamicin-macromolecule-carrying particle which process comprises covalently coupling gentamicin to a protein with a carbodiimide derivative and adsorbing or covalently coupling said gentamicin-protein moiety to inert polymer particles.
Claim 16
Dependent Claim
35 words
A process for the preparation of an agglutinable gentamicin-macromolecule-carrying particle which process comprises covalently coupling gentamicin to a protein with a carbodiimide derivative and adsorbing or covalently coupling said gentamicin-protein moiety to inert polymer particles.
Claim 17
Dependent Claim
21 words
A process as claimed in claim 13 in which the macromolecule to which gentamicin is covalently coupled is bovine serum albumin.
Claim 18
Dependent Claim
22 words
A process as claimed in claim 13 in which the protein to which the gentamicin is covalently coupled as goat gamma globulin.
Claim 19
Dependent Claim
21 words
A process as claimed in claim 13 in which the gentamicin-protein moiety is covalently coupled to inert polymer particles with bisdiazobenzidine.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-07-10 | 1982-01-09 | 1982-07-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-07-10 | 1986-01-09 | 1986-07-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-07-10 | 1990-01-09 | 1990-07-11 | $7700.00 | Window Closed / Lapsed |