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US4407952A
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Method for producing L-phenylalanine by fermentation
Assignee: Ajinomoto Co Inc
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Filing: 1980-06-16
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Granted: 1983-10-04
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Expires: 2000-06-16
Abstract
Abstract L-phenylalanine is produced by fermentation by culturing in a culture medium an L-phenylalanine producing microorganism constructed by incorporating a hybrid plasmid in a recipient strain of the genus Escherichia and recovering the L-phenylalanine which accumulates in the culture medium, said hybrid plasmid containing a deoxyribonucleic acid fragment possessing genetic information related to L-phenylalanine production and obtained from a phenylalanine analogue resistant mutant of the genus Escherichia.
Inventors
- 👤 Takayasu Tsuchida
- 👤 Konosuke Sano
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 05:10
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/52; C12N1/00; C12N1/20; C12N1/205; C12P; C12P13/00; C12P13/04; C12P13/22; C12P13/222; C12R; C12R2001/00; C12R2001/01; C12R2001/185; C12R2001/19; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/848; Y10S435/849
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
92 words
A method for producing L-phenylalanine by fermentation, which comprises: culturing in a culture medium an L-phenylalanine producing microorganism selected from the group consisting of Escherichia coli NRRL B-12141, Escherichia coli NRRL B-12145, Escherichia coli NRRL B-12146 and Escherichia coli NRRL B-12147 constructed by incorporating a hybrid plasmid in a recipient strain of the genus Escherichia and recovering the L-phenylalanine which accumulates in the culture medium, said hybrid plasmid containing a deoxyribonucleic acid fragment possessing genetic information related to L-phenylalanine production and obtained from a phenylalanine analogue resistant mutant of the genus Escherichia.
Claim 2
Independent Claim
92 words
A method for producing L-phenylalanine by fermentation, which comprises: culturing in a culture medium an L-phenylalanine producing microorganism selected from the group consisting of Escherichia coli NRRL B-12141, Escherichia coli NRRL B-12145, Escherichia coli NRRL B-12146 and Escherichia coli NRRL B-12147 constructed by incorporating a hybrid plasmid in a recipient strain of the genus Escherichia and recovering the L-phenylalanine which accumulates in the culture medium, said hybrid plasmid containing a deoxyribonucleic acid fragment possessing genetic information related to L-phenylalanine production and obtained from a phenylalanine analogue resistant mutant of the genus Escherichia.
Claim 3
Dependent Claim
19 words
The method of claim 1, wherein said culture medium contains carbon sources, nitrogen sources, inorganic ions and other nutrients.
Claim 4
Dependent Claim
17 words
The method of claim 2, wherein said carbon source is sucrose, glucose, lactose, starch hydrolysate or molasses.
Claim 5
Dependent Claim
15 words
The method of claim 2, wherein said nitrogen source is ammonia or an ammonium salt.
Claim 6
Dependent Claim
47 words
The method of claim 1, wherein said Escherichia mutant is resistant to o-, m- or p-fluorophenylalanine; o-, m- or p-aminophenylalanine; β-phenylserine, cyclohexylalanine; α-amino-β-phenylethane sulfonic acid; o-, m- or p-chlorophenylalanine; o-, m- or p-bromophenylalanine; β-2-thienylalanine; β-3-thienylalanine; β-2-furylalanine; β-2-pyrolealanine; 1-cyclopentene-1-alanine; 1-cyclohexan-1-alanine; 2-amino-4-methyl-4-hexenic acid; S-(1,2-dichlorovinyl)-cysteine; β-4-pyridylalanine; β-2-pyridylalanine, β-4-pyrazolealanine or p-nitrophenylalanine.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-10-03 | 1987-04-04 | 1987-10-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-10-03 | 1991-04-04 | 1991-10-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-10-03 | 1995-04-04 | 1995-10-04 | $7700.00 | Window Closed / Lapsed |