US3660235A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for producing phenylalanine by fermentation
Assignee: Ajinomoto Co Inc
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Filing: 1970-08-14
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Granted: 1972-05-02
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Expires: 1990-08-14
Abstract
Abstract Mutants of microorganisms of the genere Brevibacterium, Corynebacterium, Arthrobacter, Bacillus, and Candida which tolerate amounts of phenylalanine analogues sufficient to inhibit growth of the parent strains produce extracellular Lphenylalanine in conventional culture media in amounts sufficient to warrant recovery.
Inventors
- 👤 Shinji Okumura
- 👤 Shinichiro Otsuka
- 👤 Akio Yamanoi
- 👤 Fumihiro Yoshinaga
- 👤 Takeshi Honda
- 👤 Koji Kubota
- 👤 Takayasu Tsuchida
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 10:44
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/22; C12P13/222; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/83; Y10S435/832; Y10S435/839; Y10S435/84; Y10S435/843; Y10S435/911; Y10S435/921; Y10S435/923
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
16 words
A method as set forth in claim 1, wherein said mutant is Brevibacterium flavum FERM-P 422.
Claim 2
Dependent Claim
16 words
A method as set forth in claim 1, wherein said mutant is Brevibacterium lactofermentum FERM-P 423.
Claim 3
Dependent Claim
16 words
A method as set forth in claim 1, wherein said mutant is Brevibacterium lactofermentum ATCC 21420.
Claim 4
Dependent Claim
16 words
A method as set forth in claim 1, wherein said mutant is Corynebacterium acetoacidophilum ATCC 21421.
Claim 5
Dependent Claim
16 words
A method as set forth in claim 1, wherein said mutant is Arthrobacter citreus ATCC 21422.
Claim 6
Dependent Claim
16 words
A method as set forth in claim 1, wherein said mutant is Bacillus subtilis FERM-P 464.
Claim 7
Dependent Claim
16 words
A method as set forth in claim 1, wherein said mutant is Candida lipolitica FERM-P 424.
Claim 8
Dependent Claim
75 words
A method as set forth in claim 1, wherein said mutant is prepared prior to said culturing by exposing said parent strain to a mutagenic agent until mutants of said parent strain are formed, said parent strain prior to said exposing being unable to produce L-phenylalanine when cultured on said medium, and selecting from the exposed parent strain a mutant tolerant to said analogue of L-phenylalanine and capable of producing L-phenylalanine in said culture medium.
Claim 9
Dependent Claim
29 words
A method as set forth in claim 1, wherein said parent strain is a strain of Brevibacterium lactofermentum, Brevibacterium flavum, Corynebacterium acetoacidophilum, Arthrobacter citreus, Bacillus subtilis, or Candida lipolytica.
Claim 10
Dependent Claim
60 words
A method as set forth in claim 10, wherein said analogue of L-phenylalanine is Beta -amino- Beta -phenylpropionic acid, o-fluorophenylalanine, m-fluorophenylalanine, p-fluorophenylalanine, Beta -2-thienylalanine, Beta -3-thienylalanine, Beta -2-furylalanine, Beta -3-furylalanine o-aminophenylalanine, m-aminophenylalanine, p-aminophenylalanine, Alpha -amino- Beta -phenylethane sulfonate, Beta -2-pyrrolalanine, 1-cyclopentene-1-alanine, 1-cylohexene-1-alanine, Beta -4-pyridinylalanine, Beta -4-pyrazolealanine, p-nitrophenylalanine, Beta -4-thiazolealanine, cyclohexylalanine, 2-amino-4-methyl-4-hexenoic acid, S-(1,2-dichlorovinyl)-cysteine, o-chlorophenylalanine, m-chlorophenylalanine, p-chlorophenylalanine, o-bromophenylalanine, m-bromophenylalanine, or p-bromophenylalanine.
References Cited (Backward Citations)
US3562110A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-05-02 | 1975-11-01 | 1976-05-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-05-02 | 1979-11-01 | 1980-05-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-05-02 | 1983-11-01 | 1984-05-02 | $7700.00 | Window Closed / Lapsed |