US3909353A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of producing L-phenylalanine by fermentation
Assignee: Ajinomoto Co Inc
•
Filing: 1974-07-18
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Granted: 1975-09-30
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Expires: 1994-07-18
Abstract
Abstract Tyrosine-requiring mutant strains of Brevibacterium lactofermentum and Brevibacterium flavum which are capable of vigorous growth on culture media containing enough of a tryptophan analog to suppress growth of the parent strain and also capable of vigorous growth on media containing enough phenylalanine analog to suppress growth of the parent strain, produce more phenylalanine by aerobic fermentation of generally conventional aqueous media than the best phenylalanine-producing related microorganisms available heretofore.
Inventors
- 👤 Takayasu Tsuchida
- 👤 Hiroshi Matsui
- 👤 Hitoshi Enei
- 👤 Fumihiro Yoshinaga
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 04:29
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/22; C12P13/222; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/84
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
36 words
A METHOD OF PRODUCING L-PHENYLALANINE WHICH COMPRISES: A. CULTURING AN L-PHENYLALANINE-PRODUCING MUTANT OF A PARENT STRAIN OF BREVIBACTERIUM UNDER AEROBIC CONDITIONS IN AN AQUEOUS FERMENTATION MEDIUM UNTIL A SUBSTANTIAL AMOUNT OF L-PHENYLALANINE ACCUMULATES IN SAID MEDIUM
Claim 2
Independent Claim
36 words
A METHOD OF PRODUCING L-PHENYLALANINE WHICH COMPRISES: A. CULTURING AN L-PHENYLALANINE-PRODUCING MUTANT OF A PARENT STRAIN OF BREVIBACTERIUM UNDER AEROBIC CONDITIONS IN AN AQUEOUS FERMENTATION MEDIUM UNTIL A SUBSTANTIAL AMOUNT OF L-PHENYLALANINE ACCUMULATES IN SAID MEDIUM
Claim 3
Dependent Claim
26 words
SAID MUTANT BEING CAPABLE OF VIGOROUS GROWTH ON A CULTURE MEDIUM CONTAINING ENOUGH OF A TRYPTOPHAN ANALOG SUBSTANTIALLY COMPLETELT TO INHIBIT GROWTH OF SAID PARENT SRRAIN,
Claim 4
Dependent Claim
26 words
SAID MUTANT BEING CAPABLE OF VIGOROUS GROWTH ON A CULTURE MEDIUM CONTAINING ENOUGH OF A TRYPTOPHAN ANALOG SUBSTANTIALLY COMPLETELT TO INHIBIT GROWTH OF SAID PARENT SRRAIN,
Claim 5
Dependent Claim
27 words
SAID MUTANT BEING FURTHER CAPABLE OF VIGOROUS GROWTH ON A CULTURE MEDIUM CONTAINING ENOUGH OF A PHENYLALANINE ANALOG SUBSTANTIALLY COMPLETELY TO INHIBIT GROWTH OF SAID PARENT STRAIN,
Claim 6
Dependent Claim
27 words
SAID MUTANT BEING FURTHER CAPABLE OF VIGOROUS GROWTH ON A CULTURE MEDIUM CONTAINING ENOUGH OF A PHENYLALANINE ANALOG SUBSTANTIALLY COMPLETELY TO INHIBIT GROWTH OF SAID PARENT STRAIN,
Claim 7
Dependent Claim
27 words
said mutant being further capable of vigorous growth on a culture medium containing enough of a phenylalanine analog substantially completely to inhibit growth of said parent strain,
Claim 8
Dependent Claim
27 words
said mutant being further capable of vigorous growth on a culture medium containing enough of a phenylalanine analog substantially completely to inhibit growth of said parent strain,
Claim 9
Dependent Claim
20 words
A method as set forth in claim 1, wherein said mutant is a strain of Brevibacterium lactofermentum or Brevibacterium flavum.
Claim 10
Dependent Claim
18 words
A method as set forth in claim 2, wherein said mutant further requires L-tryptophan for said growth thereof.
Claim 11
Dependent Claim
18 words
said mutant requiring L-tyrosine for the growth thereof; and b. recovering the accumulated L-phenylalanine from said fermentation medium.
Claim 12
Dependent Claim
18 words
said mutant requiring L-tyrosine for the growth thereof; and b. recovering the accumulated L-phenylalanine from said fermentation medium.
Claim 13
Dependent Claim
18 words
SAID MUTANT REQUIRING L-TYROSINE FOR THE GROWTH THEREOF, AND B. RECOVERING THE ACCUMULATED L-PHENYLALANINE FROM SAID FERMENTATION MEDIUM.
Claim 14
Dependent Claim
18 words
SAID MUTANT REQUIRING L-TYROSINE FOR THE GROWTH THEREOF, AND B. RECOVERING THE ACCUMULATED L-PHENYLALANINE FROM SAID FERMENTATION MEDIUM.
Claim 15
Dependent Claim
16 words
A method as set forth in claim 3, wherein said mutant is Brevibacterium lactofermentum FERM P-2476.
Claim 16
Dependent Claim
16 words
A method as set forth in claim 2, wherein said mutant is Brevibacterium lactofermentum FERM P-1914.
Claim 17
Dependent Claim
16 words
A method as set forth in claim 2, wherein said mutant is Brevibacterium flavum FERM P-2475.
Claim 18
Dependent Claim
20 words
A method as set forth in claim 1, wherein said tryptophan analog is 5-methyltryptophan and said phenylalanine analog is p-fluorophenylalanine.
Claim 19
Dependent Claim
29 words
A method as set forth in claim 1, wherein said parent strain does not require tyrosine for the growth thereof and is incapable of producing phenylalanine during said growth.
References Cited (Backward Citations)
US3660235A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-09-29 | 1979-03-31 | 1979-09-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-09-29 | 1983-03-31 | 1983-09-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-09-29 | 1987-03-31 | 1987-09-30 | $7700.00 | Window Closed / Lapsed |