US3917511A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for preparing L-phenylalanine
Assignee: KH Neochem Co Ltd
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Filing: 1973-03-26
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Granted: 1975-11-04
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Expires: 1993-03-26
Abstract
Abstract L-phenylalanine is produced in substantial yield by fermentation from an L-phenylalanine-producing strain of Microbacterium ammoniaphilum. Specifically mutated strains of the above noted bacteria that exhibit resistance to at least one compound selected from the group consisting of tyrosine, phenylalanine and analogues thereof, produce large amounts of L-phenylalanine when cultured in a suitable nutrient medium.
Inventors
- 👤 Kiyoshi Nakayama
- 👤 Hiroshi Hagino
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 08:57
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/22; C12P13/222; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
60 words
A PROCESS FOR PRODUCING L-PHENYLALANINE BY FERMENTATION WHICH COMPRISES CULTURING IN A NUTRIENT MEDIUM AN LPHENYLALANINE-PRODUCING MUTANT STRAIN OF MICROBACTERIUM AMMONIAPHILUM HAVING THE PROPERTY OF RESISTANCE TO AT LEAST ONE COMPOUND SELECTED FROM THE GROUP CONSISTING OF TYROSINE, PHENYLALANINE AND ANALOGUES THEREOF TO PRODUCE AND ACCUMULATE L-PHENYLALANINE IN THE CULTURE LIQUOR, AND THEREAFTER RECOVERING THE L-PHENYLALANINE FROM THE CULTURE LIQUOR..
Claim 2
Dependent Claim
60 words
A PROCESS FOR PRODUCING L-PHENYLALANINE BY FERMENTATION WHICH COMPRISES CULTURING IN A NUTRIENT MEDIUM AN LPHENYLALANINE-PRODUCING MUTANT STRAIN OF MICROBACTERIUM AMMONIAPHILUM HAVING THE PROPERTY OF RESISTANCE TO AT LEAST ONE COMPOUND SELECTED FROM THE GROUP CONSISTING OF TYROSINE, PHENYLALANINE AND ANALOGUES THEREOF TO PRODUCE AND ACCUMULATE L-PHENYLALANINE IN THE CULTURE LIQUOR, AND THEREAFTER RECOVERING THE L-PHENYLALANINE FROM THE CULTURE LIQUOR..
Claim 3
Dependent Claim
15 words
The process of claim 1 in which said mutant strain is Microbacterium ammoniaphilum ATCC 21645.
Claim 4
Dependent Claim
21 words
The process of claim 1 in which the nutrient medium includes a nitrogen source, a carbon source and inorganic nutrient materials.
Claim 5
Dependent Claim
36 words
The process of claim 1 in which the culturing is carried out aerobically at a temperature of from about 20*C to about 40*C at a pH of from 4 to 8 for 2 to 5 days.
References Cited (Backward Citations)
US3660235A
Cited
US3759790A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-11-03 | 1979-05-05 | 1979-11-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-11-03 | 1983-05-05 | 1983-11-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-11-03 | 1987-05-05 | 1987-11-04 | $7700.00 | Window Closed / Lapsed |