Patent US4394444A successfully refreshed from Google Patents.
US4394444A
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US
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Cofactor indicator compositions
Assignee: Bayer Corp
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Filing: 1982-06-21
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Granted: 1983-07-19
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Expires: 2002-06-21
Abstract
Abstract A composition, test device, method of making a test device and process for determination of an analyte in a liquid sample are disclosed. More particularly, there is provided a composition for the determination of an analyte in a sample which composition comprises an analyte-responsive component comprising a pyridine nucleotide susceptible of reduction in response to the presence of said analyte and at least one constituent interreactive with said analyte to cause reduction of the pyridine nucleotide, an uncoupler effective to generate an oxidizing substance from the reduced form of said pyridine nucleotide, a peroxidatively active substance, and an indicator which, when oxidized, is not susceptible to reduction by said reduced pyridine nucleotide. The pyridine nucleotide can be nicotine adenine dinucleotide (NAD) or nicotine adenine dinucleotide phosphate (NADP).
Inventors
- 👤 Erma C. Cameron
- 👤 Claude R. Gunter
- 👤 Rodric H. White-Stevens
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 16:37
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/60; C12Q1/008; C12Q1/26; C12Q1/54; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81
Claims Analysis
Total Claims: 37
Claim 1
Independent Claim
111 words
A composition for the determination of an analyte in a sample which composition comprises: an analyte-responsive component comprising a pyridine nucleotide susceptible of reduction in response to the presence of said analyte and at least one constituent interreactive with said analyte to cause reduction of the pyridine nucleotide; a hydroxylase capable of being uncoupled and a pseudosubstrate capable of uncoupling said hydroxylase, which hydroxylase and pseudosubstrate, in the presence of the reduced form of said pyridine nucleotide, are effective to generate hydrogen peroxide; a peroxidatively active substance; and a hydrazone indicator comprising a hydrazone and a coupler, which indicator, when in its oxidized form, cannot be reduced by said pyridine nucleotide.
Claim 2
Dependent Claim
111 words
A composition for the determination of an analyte in a sample which composition comprises: an analyte-responsive component comprising a pyridine nucleotide susceptible of reduction in response to the presence of said analyte and at least one constituent interreactive with said analyte to cause reduction of the pyridine nucleotide; a hydroxylase capable of being uncoupled and a pseudosubstrate capable of uncoupling said hydroxylase, which hydroxylase and pseudosubstrate, in the presence of the reduced form of said pyridine nucleotide, are effective to generate hydrogen peroxide; a peroxidatively active substance; and a hydrazone indicator comprising a hydrazone and a coupler, which indicator, when in its oxidized form, cannot be reduced by said pyridine nucleotide.
Claim 3
Dependent Claim
13 words
The composition of claim 1 wherein said pyridine nucleotide is nicotine adenine dinucleotide.
Claim 4
Dependent Claim
14 words
The composition of claim 1 wherein said pyridine nucleotide is nicotine adenine dinucleotide phosphate.
Claim 5
Dependent Claim
16 words
The composition of claim 1 wherein said at least one interreactive constitutent is an analyte-specific enzyme.
Claim 6
Dependent Claim
11 words
The composition of claim 4 wherein said enzyme is a dehydrogenase.
Claim 7
Dependent Claim
11 words
The composition of claim 5 wherein said enzyme is lactate dehydrogenase.
Claim 8
Dependent Claim
11 words
The composition of claim 5 wherein said enzyme is β-hydroxybutyrate dehydrogenase.
Claim 9
Dependent Claim
11 words
The composition of claim 5 wherein said enzyme is α-hydroxybutyrate dehydrogenase.
Claim 10
Dependent Claim
11 words
The composition of claim 5 wherein said enzyme is alcohol dehydrogenase.
Claim 11
Dependent Claim
12 words
The composition of claim 5 wherein said enzyme is a steroid dehydrogenase.
Claim 12
Dependent Claim
11 words
The composition of claim 10 wherein said enzyme is cholesterol dehydrogenase.
Claim 13
Dependent Claim
11 words
The composition of claim 5 wherein said enzyme is glucose dehydrogenase.
Claim 14
Dependent Claim
33 words
The composition of claim 1 wherein said at least one interreactive constituent is an analyte-specific enzyme and an enzyme interreactive with the product of the reaction of said analyte-specific enzyme with the analyte.
Claim 15
Dependent Claim
31 words
The composition of claim 13 wherein said analyte-responsive enzyme is a lipase and said enzyme interreactive with the product of the reaction of said lipase with the analyte is glycerol dehydrogenase.
Claim 16
Dependent Claim
30 words
The composition of claim 13 wherein said analyte-specific enzyme is hexokinase and said enzyme interreactive with the product of the reaction of said hexokinase with the analyte is glucose-6-phosphate dehydrogenase.
Claim 17
Dependent Claim
15 words
The composition of claim 1 wherein said interreactive component is a substrate of the analyte.
Claim 18
Dependent Claim
13 words
The composition of claim 16 wherein said interreactive component is a reduced substrate.
Claim 19
Dependent Claim
11 words
The composition of claim 17 wherein said interreactive component is lactate.
Claim 20
Dependent Claim
12 words
The composition of claim 17 wherein said interreactive component is β-hydroxybutyric acid.
Claim 21
Dependent Claim
12 words
The composition of claim 17 wherein said interreactive component is α-hydroxybutyric acid.
Claim 22
Dependent Claim
11 words
The composition of claim 17 wherein said interreactive component is ethanol.
Claim 23
Dependent Claim
12 words
The composition of claim 17 wherein said interreactive component is a steroid.
Claim 24
Dependent Claim
11 words
The composition of claim 22 wherein said interreactive component is cholesterol.
Claim 25
Dependent Claim
11 words
The composition of claim 17 wherein said interreactive component is glucose.
Claim 26
Dependent Claim
33 words
The composition of claim 1 wherein said at least one interreactive constituent is an analyte-specific substrate and an enzyme interreactive with the product of the reaction of said analyte-specific substrate with the analyte.
Claim 27
Dependent Claim
31 words
The composition of claim 25 wherein said analyte-specific substrate is a triglyceride and said enzyme interreactive with the product of the reaction of said triglyceride with the analyte is glycerol dehydrogenase.
Claim 28
Dependent Claim
30 words
The composition of claim 25 wherein said analyte-specific substrate is glucose and said enzyme interreactive with the product of the reaction of said glucose with the analyte is glucose-6-phosphate dehydrogenase.
Claim 29
Dependent Claim
21 words
The composition of claim 1 wherein said hydroxylase capable of being uncoupled is salicylate hydroxylase and said pseudosubstrate is a benzoate.
Claim 30
Dependent Claim
20 words
The composition of claim 1 wherein said hydroxylase capable of being uncoupled is p-hydroxybenzoate hydroxylase and said pseudosubstrate is 6-hydroxynicotinate.
Claim 31
Dependent Claim
13 words
The composition of claim 1 wherein said peroxidatively active substance is a peroxidase.
Claim 32
Dependent Claim
11 words
The composition of claim 1 wherein said hydrazone is 3-methyl-2-benzothiazolinone hydrazone.
Claim 33
Dependent Claim
10 words
The composition of claim 31 wherein said coupler is primaquine.
Claim 34
Dependent Claim
22 words
A device for the determination of an analyte in a sample which comprises a carrier incorporated with the composition of claim 1.
Claim 35
Dependent Claim
29 words
A method for the determination of an analyte in a sample which comprises contacting said sample with the device of claim 33 and observing any resultant color change thereon.
Claim 36
Dependent Claim
26 words
A process for preparing a device for the determination of an analyte in a sample which comprises incorporating a carrier with the composition of claim 1.
Claim 37
Dependent Claim
28 words
A method for the determination of an analyte in a sample which comprises contacting said sample with the composition of claim 1 and observing any resultant color change.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-07-18 | 1987-01-17 | 1987-07-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-07-18 | 1991-01-17 | 1991-07-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-07-18 | 1995-01-17 | 1995-07-19 | $7700.00 | Window Closed / Lapsed |