Triglyceride hydrolysis and assay
Abstract
Abstract GLYCEROL ESTERS PRESENT IN AQUEOUS MEDIA, SUCH AS SERUM TRIGLYCERIDE AND MILK FAT, ARE ASSAYED BY COMPLETE ENZYMATIC HYDROLYSIS USING BOTH A LIPASE AND A PROTEASE, WHEREUPON THE LIBERATED GLYCEROL IS DETERMINED. A NUMBER OF ALTERNATIVE ENZYMATIC PROCEDURES ARE PROVIDED FOR THE GLYCEROL ASSAY, ALL IN THE ORGINAL AQUEOUS MEDIUM, SUCH AS CONVERSION TO GLYCEROL-1-PHOSPHATE WITH GLYCEROL KINASE; CONVERSION OF ATP TO ADP BY THE SAME RECTION FOLLOWED BY CONVERSION OF THE ADP PLUS PHOSPHOENOLPYRUVATE USING PYRUVATE KINASE TO ATP AND PYRUVATE ION FOLLOWED BY DETERMINING THE LATTER; AND ALTERNATIVELY CONVERTING THE PYRUVATE ION SO FORMED WITH NADH USING LACTATE DEHYDROGENASE TO LACTATE ION AND NAD AND DETERMINING THE LATTER BY OPTICAL MEASUREMENT.
Inventors
- 👤 Giovanni Bucolo
- 👤 Harold David
Bibliographic Details
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