US3384555A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Direct fluorometric method for measuring dehydrogenase activity
Assignee: United States Department of the Army
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Filing: 1965-11-30
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Granted: 1968-05-21
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Expires: 1985-11-30
Abstract
No abstract record available.
Inventors
- 👤 George G Guilbault
- 👤 David N Kramer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 19:40
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/32
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
93 words
FLUOROMETRIC METHOD FOR THE QUANTITATIVE DETERMINATION OF A DEHYDROGENASE WHICH COMPRISES: (A) PLACING KNOWN AMOUNTS OF REAZURIN, DIAPHORASE, NICOTINAMIDE ADENINE DINUCLETIDE AND A SUBSTRATE COMPRISING A MEMBER OF THE GROUP CONSISTING OF ALKALI METAL SALTS OF MALIC, LACTIC, AND GLUTAMIC ACIDS, ETHANOL, GLCOSE-6-PHOSPHATE, GLYCEROL PHOSPHATE, (-)-A-GLYCEROL PHOSPHATE IN A FLUORESCENCE CELL OF A SPECTROPHOTOFLUOROMETER AT A BUFFERED PH IN THE RANGE 8.0-9.0; (B) ADDING AN UNKNOWN CONCENTRATION OF A DEHYDROGENASE CORRESPONDING TO ITS SUBSTRATE AT ZERO TIME; (C) MEASURING THE CHANGE IN FLUORESCENCE WITH TIME TO DETERMINE THE CONCENTRATION OF DEHYDROGENASE ORIGINALLY PRESENT.
Claim 2
Dependent Claim
93 words
FLUOROMETRIC METHOD FOR THE QUANTITATIVE DETERMINATION OF A DEHYDROGENASE WHICH COMPRISES: (A) PLACING KNOWN AMOUNTS OF REAZURIN, DIAPHORASE, NICOTINAMIDE ADENINE DINUCLETIDE AND A SUBSTRATE COMPRISING A MEMBER OF THE GROUP CONSISTING OF ALKALI METAL SALTS OF MALIC, LACTIC, AND GLUTAMIC ACIDS, ETHANOL, GLCOSE-6-PHOSPHATE, GLYCEROL PHOSPHATE, (-)-A-GLYCEROL PHOSPHATE IN A FLUORESCENCE CELL OF A SPECTROPHOTOFLUOROMETER AT A BUFFERED PH IN THE RANGE 8.0-9.0; (B) ADDING AN UNKNOWN CONCENTRATION OF A DEHYDROGENASE CORRESPONDING TO ITS SUBSTRATE AT ZERO TIME; (C) MEASURING THE CHANGE IN FLUORESCENCE WITH TIME TO DETERMINE THE CONCENTRATION OF DEHYDROGENASE ORIGINALLY PRESENT.
References Cited (Backward Citations)
US2999052A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1971-05-21 | 1971-11-20 | 1972-05-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1975-05-21 | 1975-11-20 | 1976-05-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1979-05-21 | 1979-11-20 | 1980-05-21 | $7700.00 | Window Closed / Lapsed |